Growth rate of growing pigs is weakly correlated genetically with litter size,while the amount of genetic variation for growth rate changes with litter size

Abstract

We reasoned that if we assessed pigs for litter size and growth rate during the grow-out stage of production (25–100 kg body weight) we would find: 1) an unfavourable genetic correlation between litter size and growth rate; and 2) that the amount of additive genetic variation expressed for growth rate varies with litter size. We tested these premises by assessing 2212 litters from the Yorkshire breed for litter size, while 3038 growing pigs from 550 of these litters were assessed for growth rate during the grow-out stage of production. Genetic correlations were estimated using the additive genetic (co)variances obtained from a bivariate linear animal model fitted to litter size and growth rate. The amount of additive genetic variation expressed for growth rate as a function of litter size was estimated by fitting a univariate linear animal model with random regression on litter size. Our findings did not support the first of our premises as we found that the genetic correlation between litter size and growth rate was favourable (0.28±0.27), albeit not significantly different from zero. However, we were able to support our second premise as we found that the relationship between amount of additive genetic variation for growth rate and litter size was quadratic; the amount of additive genetic variation was highest in small and large litters (h²=0.60 and 0.65), and lowest in intermediate litter sizes (h²=0.29). These findings indicate that: 1) breeding for litter size would not reduce the growth rate of growing pigs; and 2) the amount of genetic variation for growth rate changes with litter size.     

Validation of the suppressive subtractive hybridization method in Mycoplasma agalactiae species by the comparison of a field strain with the type strain PG2

The subtractive suppressive hybridization (SSH), a method that allows the identification of sequences that are present in one genome (tester) but not in the other (driver), is a promising technique for the comparison of Mycoplasma agalactiae pathogenic strains. The optimal conditions for SSH were established by subtracting the M. agalactiae type strain PG2 DNA from the M. agalactiae strain 5632 DNA. Because these two strains possess different vpma gene repertoires, 5632-specific vpma sequences (and possibly other 5632-specific sequences) were predicted to be retrieved by SSH. The subtracted tester DNA was PCR-amplified and cloned into the pGEM-T easy E. coli vector. Two independent libraries were generated and used to prepare individual probes that were tested by Southern blot with genomic DNA from various field isolates and mycoplasma reference strains. Sequence analysis of two overlapping clones showed that they potentially code for a large carboxyterminal portion of a new vpma ORF. Several DNA fragments homologous to insertion sequences were also found in 5632 and related strains. These preliminary data suggest that SSH is a powerful method to investigate differences between mycoplasma strains, and may be applied to molecular epidemiology, diagnostic, and host specificity or pathogenicity determinant discovery.     

Wildfires and landscape patterns in the Eastern Iberian Peninsula

The relations between disturbance regime and landscape patterns have been developed from a theoretical perspective, but few studies have tested these relations when forces promoting opposing heterogeneity patterns are simultaneously operating on a landscape. This work provides quantitative evidence of these relations in areas dominated by human activity, showing that landscape heterogeneity decreases disturbance spread. In turn, disturbance introduces a source of landscape heterogeneity, but it is not enough to counterbalance the homogeneity trend due to agricultural abandonment. Land cover changes and wildfire occurrence (fires larger than 0.3 km²) have been monitored in the Tivissa municipality (208.4 km²) (Catalonia, NE Spain) from 1956 to 1993. Land cover maps were obtained from 1956, 1978 and 1993 and they were overlaid with fire occurrence maps obtained for the 1975–1995 period from 60 m resolution remote sensing images, which allow the identification of burned areas by sudden drops in Normalized Difference Vegetation Index (NDVI). Changes in landscape patterns in relation to fire regime have been analyzed considering several parameters: patch density, mean patch size, mean distance to the nearest neighbour of the same category, edge density, and the Shannon diversity index. In the 1956–1993 period there is a trend to increasing landscape homogenization due to the expansion of shrub­lands linked to a decrease in forest surface, and to the abandonment of agricultural lands. This trend, however, is not constant along all the period. Fires are more likely to occur in woody, homogenous areas, increasing landscape heterogeneity, as observed in the 1978–1993 period. This increase in heterogeneity does not counterbalance the general trend to landscape homogenization as a consequence of agricultural abandonment and the coalescence of natural vegetation patches.This revised version was published online in May 2005 with corrections to the Cover Date.     

Coupling landscape graph modeling and biological data: a review

Landscape Ecology - Landscape graphs are widely used to model networks of habitat patches. As they require little input data, they are particularly suitable for supporting conservation decisions...     

Spatial and temporal variations in leaf area index, specific leaf area and leaf nitrogen of two co-occurring savanna tree species

Foliage growth, mass- and area-based leaf nitrogen concentrations (Nm and N a) and specific leaf area (SLA) were surveyed during a complete vegetation cycle for two co-occurring savanna tree species: Crossopteryx febrifuga (Afzel. ex G. Don) Benth. and Cussonia arborea A. Rich. The study was conducted in the natural reserve of Lamto, Ivory Coast, on isolated and clumped trees. Leaf flush occurred before the beginning of the rainy season. Maximum leaf area index (LAI), computed on a projected canopy basis for individual trees, was similar (mean of about 4) for both species. Seasonal courses of the ratio of actual to maximum LAI were similar for individuals of the same species, but differed between species. For C. febrifuga, clumped trees reached their maximum LAI before isolated trees. The LAI of C. arborea trees did not differ between clumped and isolated individuals, but maximum LAI was reached about 2 months later than for C. febrifuga. Leaf fall was associated with decreasing soil water content for C. arborea. For C. febrifuga, leaf fall started before the end of the rainy period and was independent of changes in soil water content. These features lead to a partial niche separation in time for light resource acquisition between the two species. Although Nm, N a and SLA decreased with time, SLA and N a decreased later in the vegetation cycle for C. arborea than for C. febrifuga. For both species, N a decreased and SLA increased with decreasing leaf irradiance within the canopy, although effects of light on leaf characteristics did not differ between isolated and clumped trees. Given relationships between N a and photosynthetic capacities previously reported for these species, our results show that C. arborea exhibits higher photosynthetic capacity than C. febrifuga during most of the vegetation cycle and at all irradiances.     

An assessment of caprine tuberculosis prevalence in Lubumbashi slaughterhouse,Democratic Republic of Congo

Tropical Animal Health and Production - Caprine tuberculosis is a major health problem for goats and a major zoonosis of veterinary public health interest. In order to prepare a response to and...     

Feed handling of lactating crossbred cows maintained in a semi‐arid region during the hot season: physiological parameters,ingestive behavior and performance

The effects of time of feed delivery (14.00 hours; 14.00 and 20.00 hours; 16.00 and 18.00 hours) on the physiological parameters, ingestive behavior, nutrient intake and production of lactating cows maintained in a semi‐arid region during the hot season were evaluated. Regardless of treatment, all animals received the first feeding supply at 06.00 hours. Eight cows with an average body weight of 600 kg, average milk yield of 20 kg/day and 80 days in milk were utilized. The rectal temperature, respiratory rate and sweating rate were not affected (P > 0.05), with average values of 38.5°C, 53.8 movements/min and 104 g/m²/h respectively. There was no effect (P > 0.05) on the eating time (314 min/day), ruminating time (564 min/day), drinking time (61 min/day) and idle time (502 min/day). Similarly, the intake of nutrients and performance of lactating cows were not affected (P > 0.05), with average dry matter intake of 19.8 kg/day, 4% fat‐corrected milk of 20.6 kg/day and milk fat concentration of 4.03 g/100 g. Since the behavior and performance have not altered, any times of feed delivery evaluated could be used to crossbred Holstein × Zebu cows maintained on a feedlot in semi‐arid regions during the hot season.     

High lysyl oxidase expression is an indicator of poor prognosis in dogs with cutaneous mast cell tumours

Mast cell tumour (MCT) is one of the most frequent skin tumours in dogs. Due to their unpredictable biological behaviour, MCTs often cause several therapeutic frustrations, leading to investigation regarding prognostic markers. Lysyl oxidase (LOX) is an enzyme that promotes extracellular matrix stability and contributes to cell migration, angiogenesis and epithelial-mesenchymal transition. Its expression positively correlates with poor prognoses in several human and canine mammary cancers. The aim of this study was to characterise the immunohistochemical expression of LOX in MCT samples and compare it with histological grading and post-surgical survival. Twenty-six tumours were submitted to immunohistochemistry for LOX expression evaluation. All samples were positive for LOX, with variable percentages of cytoplasmic and nuclear positivity. Cytoplasmic positivity was significantly higher in high-grade MCTs (P = .0297). Our results indicate that high expression of cytoplasmic LOX in neoplastic mast cells is an indicator of poor prognosis for canine cutaneous MCTs.     

Les marqueurs lymphocytaires chez les animaux domestiques—I. Lectines mitogenes et lectines non mitogenes

The authors reviewed the lymphocyte markers in domestic animals. The first part is devoted to lectins. The general and methodologic aspects of lectins as mitogens are studied. The main mitogen lectins are emphasized in regard to their field of utilisation and the technical aspect of their use. The non-mitogen lectins are presented for cell suspension or tissue section staining.