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761.
Benjamin S 《Science (New York, N.Y.)》2000,290(5500):2273-2274
Quantum cryptography offers the potential of totally secure transfer of information, but as Benjamin discusses in this Perspective, its practical implementation hinges on being able to generate single photons (rather than two or more) at a time. Michler et al. show how this condition can be met in a quantum dot microdisk structure. Single molecules were also recently shown to allow controlled single-photon emission. 相似文献
762.
The temporal evolution of past martian surface temperatures is poorly known. We used thermochronology and published noble gas and petrographic data to constrain the temperature histories of the nakhlites and martian meteorite ALH84001. We found that the nakhlites have not been heated to more than 350 degrees C since they formed. Our calculations also suggest that for most of the past 4 billion years, ambient near-surface temperatures on Mars are unlikely to have been much higher than the present cold (<0 degrees C) state. 相似文献
763.
Ramachandran N Hainsworth E Bhullar B Eisenstein S Rosen B Lau AY Walter JC LaBaer J 《Science (New York, N.Y.)》2004,305(5680):86-90
Protein microarrays provide a powerful tool for the study of protein function. However, they are not widely used, in part because of the challenges in producing proteins to spot on the arrays. We generated protein microarrays by printing complementary DNAs onto glass slides and then translating target proteins with mammalian reticulocyte lysate. Epitope tags fused to the proteins allowed them to be immobilized in situ. This obviated the need to purify proteins, avoided protein stability problems during storage, and captured sufficient protein for functional studies. We used the technology to map pairwise interactions among 29 human DNA replication initiation proteins, recapitulate the regulation of Cdt1 binding to select replication proteins, and map its geminin-binding domain. 相似文献
764.
Ma'ayan A Jenkins SL Neves S Hasseldine A Grace E Dubin-Thaler B Eungdamrong NJ Weng G Ram PT Rice JJ Kershenbaum A Stolovitzky GA Blitzer RD Iyengar R 《Science (New York, N.Y.)》2005,309(5737):1078-1083
We developed a model of 545 components (nodes) and 1259 interactions representing signaling pathways and cellular machines in the hippocampal CA1 neuron. Using graph theory methods, we analyzed ligand-induced signal flow through the system. Specification of input and output nodes allowed us to identify functional modules. Networking resulted in the emergence of regulatory motifs, such as positive and negative feedback and feedforward loops, that process information. Key regulators of plasticity were highly connected nodes required for the formation of regulatory motifs, indicating the potential importance of such motifs in determining cellular choices between homeostasis and plasticity. 相似文献
765.
766.
Benjamin M 《Science (New York, N.Y.)》1917,45(1162):337-338
767.
Sieber JJ Willig KI Kutzner C Gerding-Reimers C Harke B Donnert G Rammner B Eggeling C Hell SW Grubmüller H Lang T 《Science (New York, N.Y.)》2007,317(5841):1072-1076
Most plasmalemmal proteins organize in submicrometer-sized clusters whose architecture and dynamics are still enigmatic. With syntaxin 1 as an example, we applied a combination of far-field optical nanoscopy, biochemistry, fluorescence recovery after photobleaching (FRAP) analysis, and simulations to show that clustering can be explained by self-organization based on simple physical principles. On average, the syntaxin clusters exhibit a diameter of 50 to 60 nanometers and contain 75 densely crowded syntaxins that dynamically exchange with freely diffusing molecules. Self-association depends on weak homophilic protein-protein interactions. Simulations suggest that clustering immobilizes and conformationally constrains the molecules. Moreover, a balance between self-association and crowding-induced steric repulsions is sufficient to explain both the size and dynamics of syntaxin clusters and likely of many oligomerizing membrane proteins that form supramolecular structures. 相似文献
768.
769.
The circuitry and function of mammalian visual cortex are shaped by patterns of visual stimuli, a plasticity likely mediated by synaptic modifications. In the adult cat, asynchronous visual stimuli in two adjacent retinal regions controlled the relative spike timing of two groups of cortical neurons with high precision. This asynchronous pairing induced rapid modifications of intracortical connections and shifts in receptive fields. These changes depended on the temporal order and interval between visual stimuli in a manner consistent with spike timing-dependent synaptic plasticity. Parallel to the cortical modifications found in the cat, such asynchronous visual stimuli also induced shifts in human spatial perception. 相似文献
770.