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31.
Takeru Kiuchi Kenichi Watanabe Shotaro Nakagun Kazurou Miyahara Noriyuki Horiuchi Yoshiyasu Kobayashi 《Journal of toxicologic pathology》2022,35(1):83
A Chihuahua dog showed persistent itching in the right ear canal. Anti-inflammatory medicines and prednisolone were ineffective and total ear canal ablation was performed. Histological diagnosis was chronic otitis externa. Eosinophilic intranuclear inclusion bodies (Cowdry type A and full-type) were occasionally observed in the ceruminous gland epithelium. The inclusion bodies were negative for nucleic acid and ultrastructurally composed of fibrous structures (approximately 10 nm in width). Viral infection was initially suspected, but polymelase chain reaction tests did not detect the expected viral genes. Immunohistochemistry revealed that the inclusion bodies were positive for heat shock protein 70 (HSP70), suggesting that these bodies could be protein aggregates including HSP70. The etiology of this lesion has not been elucidated, but chronic inflammation may influence the cytoplasm-to-nuclear transportation of HSP70. To the best of our knowledge, this is the first report of canine chronic otitis externa with HSP70-positive intranuclear inclusion bodies. 相似文献
32.
Okajima Kenji Nagaoka Seiya Islam Mohammad Raihanul Ito Ryouei Watanabe Ken 《Paddy and Water Environment》2020,18(2):345-353
Paddy and Water Environment - Repairs of concrete irrigation channels in Japan are guided to a large extent by the degree to which their walls have degraded over decades of use. Current methods of... 相似文献
33.
The flammability of shrubs and trees in anAcacia mangium plantation based on silica-free ash content
Preliminary research to understand the flammability of 14 species of shrubs and 8 species of trees based on silica-free ash
content was done in order to recognize which shrubs or trees are more flammable. The results show that the silica-free ash
content of shrubs and trees leaves was greater than the stems ranging between 1.7% and 11.4% for leaf and 0.4% and 7.8% for
the stems. The shrubsDicranopteris linearis, Imperata cylindrica, Eupatorium pubescens, Lantana camara, Eugenia sp.,Cliforia laurifolia, Pterospermum sp.,Hibiscus similis, Clidemia hirta andTrema orientalis must be considered when fire invades the plantation as well as the treeParaserianthes falcataria, Eucalyptus urophylla, Calliandra callothyrsus, andPeronema canescens. 相似文献
34.
ABSTRACT: Hermatypic (reef-building) corals harbor dinoflagellate endo-symbionts Symbiodinium spp. In studying gene expression in such symbiotic corals, problems arise regarding how to distinguish the coral and symbiont mRNA, and how to estimate their fractions in the mRNA population of the holobiont (symbiotic complex of the coral and Symbiodinium cells). In this study, these issues were addressed using juveniles of hermatypic coral Acropora tenuis in symbiosis with Symbiodinium cells of strain PL-TS-1. First, the guanine-cytosine (GC) contents were determined in expressed sequence tags (EST) from PL-TS-1 cells cultured in vitro and symbiont-free larvae of A. tenuis , and their average GC contents were found to be significantly different. The average GC content of the EST from the holobiont was much closer to that of A. tenuis larvae, suggesting that the majority (>90%) of mRNA isolated from the holobiont originated in the host. In protein-coding sequences, little overlap was observed between the GC-content distributions of PL-TS-1 cells and A. tenuis larvae. All of the coding sequences ( n = 59) found in the A. tenuis EST had GC contents below 0.5, whereas the GC content exceeded 0.5 in the majority (43/44) of coding sequences from the nuclear genome of PL-TS-1 cells. 相似文献
35.
Nakagawa Koki Murase Jun Asakawa Susumu Watanabe Takeshi 《Journal of Soils and Sediments》2020,20(11):4034-4041
Journal of Soils and Sediments - To reveal whether microaerophilic Fe(II)-oxidizing bacteria (FeOB) participate in the Fe(II) oxidation at the oxic-anoxic interface in flooded paddy field soil,... 相似文献
36.
I. Watanabe 《Soil biology & biochemistry》1977,9(2):99-103
Soil samples from field plots that had received annual applications of pentachlorophenol (PCP) for 3 consecutive years were analyzed each year for changes in microbial populations, particularly in PCP-decomposing microorganisms and PCP-tolerant bacteria. Within 6 weeks after an initial application of PCP, PCP-decomposing microorganisms increased by about 3 orders of magnitude; this increased population was still observed the following spring. The number of PCP-decomposing microorganisms in the soils that received PCP for 3 yr reached 105· g?1 soil or more, while that in soils without PCP was 102· g?1. The soils that received PCP were not enriched with tetrachlorophenoldecomposing microorganisms. The microbes enriched by PCP may have been bacterial in nature. In 1972 and 1974, the number of PCP-tolerant bacteria increased immediately after PCP application and continued to increase for 2 weeks, then declined. The 1973 data, however, showed no such decline. The number of PCP-tolerant and of crystal violet-tolerant (Gram-negative) bacteria correlated fairly well except at 2 weeks after PCP application (when PCP-tolerant bacteria were more numerous). 相似文献
37.
Acetyle reduction and 15N studies showed that the addition of sulfate to flooded soil with rice straw enhanced N2 fixation. The extent of the enhancement was dependent on the sulfate concentration. Sulfate also increased the population of SO42? reducing bacteria and was completely reduced to sulfide by those microorganisms. Purified cultures of soil isolates were capable of C2H2 reduction. Based on this evidence, SO42? reducing bacteria were considered responsible for the increase in N2 fixation but only 1–2 mg N2 were fixed g1? SO42? reduced. We conclude that the contribution of SO42? reducing bacteria to the total N2 fixation in flooded soil is unimportant. 相似文献
38.
Akihiko Yatsu Tomowo Watanabe Junta Mori Kazuya Nagasawa Yukimasa Ishida Toshimi Meguro Yoshihiko Kamei & Yasunori Sakurai 《Fisheries Oceanography》2000,9(2):163-170
Variability in catch-per-unit-effort (CPUE) was examined for the autumn cohort of Ommastrephes bartramii collected with research driftnets during 1979–1998 along five longitudinal transects passing through the Subarctic, Transitional and Subtropical Domains in the North Pacific. CPUE was generally low during the period of intensive commercial driftnet fishing (1980–1992) and increased following the 1992 moratorium on the use of large-scale driftnets. However, CPUE levels were low for the cohorts hatched in 1992 and 1996 (captured in subsequent years owing to a one-year life of O. bartramii ) that experienced low sea surface temperatures from hatching to recruitment. Among similar-aged squid collected from 180° and 179°30'W in June, mantle lengths were significantly greater in 1997 than during 1995–96. These findings suggest that the driftnet fishery and sea surface temperatures from hatching to recruitment strongly affected stock abundance and possibly growth. 相似文献
39.
In order to determine the effects of ghost fishing by lost gill nets, the relationship was examined between soak time and
number of enmeshed animals in experimentally lost gill nets by using diving observations. Two experimental gill nets were
set at 13 m depth in Tateyama Bay, Chiba Prefecture, Japan for approximately 200 days. One gill net was deployed in a small
trough surrounded by artificial reefs, and the other was deployed on an adjacent open sandy bed. Twelve species of crustaceans,
six species of gastropods, and five species of bony fish were enmeshed in the experimental gill nets. The number of enmeshed
animals in the artificial reef gill net was substantially larger than that in the sandy bed gill net. The number of enmeshed
animals in the experimental gill nets increased rapidly within one month after deployment, and then declined gradually showing
fluctuations caused by the decrease in newly enmeshed animals, and the drop off from gill nets caused by the decomposition
of dead animals. The decrease in the number of enmeshed animals was expressed by logarithmic equations, and based on these
equations, the duration of capture function for the lost gill nets was calculated to be 284–561 days in the artificial reef
gill net and 200 days in the sandy bed gill net. The duration of capture function for the lost gill nets for non-commercial
by-catch species such as small crustaceans and gastropods was longer than for commercial species such as Japanese spiny lobster
and bony fish. 相似文献
40.
Edgar C Amar Viswanath Kiron Shuichi Satoh Nobuaki Okamoto and Takeshi Watanabe 《Fisheries Science》2000,66(6):1068-1075
SUMMARY: We attempt to assess the impact of different levels of dietary β-carotene on immune function in rainbow trout. Semi-purified diets containing 0, 40, 200, and 400 mg β-carotene/kg dry diet were fed for 12 weeks to fish with average weight of 45 g. In addition to the humoral and cellular immune parameters, growth and feed utilization were examined. There were no marked differences in growth and feed utilization showing that β-carotene was not particularly efficient in enhancing growth of rainbow trout. Of the immune parameters measured, total immunoglobulin was significantly highest for the 200 mg β-carotene fed group. Serum complement activity (alternate pathway) at 200 and 400 mg β-carotene supplementation was significantly higher than that of the unsupplemented group. An increasing trend in lysozyme activity was observed, however, the differences among the groups were not significant. Phagocytic activity was similar among diet groups except at the highest level of supplementation where it was the maximum. Oxygen radical production by peripheral blood leukocytes appeared to be lower at higher levels of carotenoid supplementation. Overall, dietary β-carotene clearly enhanced immune response parameters in rainbow trout such as serum complement activity and total plasma immunoglobulin but did not show a definite influence for the other factors examined in the present study. 相似文献