全文获取类型
收费全文 | 151篇 |
免费 | 14篇 |
专业分类
林业 | 10篇 |
农学 | 27篇 |
基础科学 | 2篇 |
45篇 | |
综合类 | 12篇 |
农作物 | 5篇 |
水产渔业 | 5篇 |
畜牧兽医 | 37篇 |
园艺 | 5篇 |
植物保护 | 17篇 |
出版年
2023年 | 5篇 |
2022年 | 5篇 |
2021年 | 4篇 |
2020年 | 4篇 |
2019年 | 5篇 |
2018年 | 5篇 |
2017年 | 14篇 |
2016年 | 5篇 |
2015年 | 9篇 |
2014年 | 6篇 |
2013年 | 19篇 |
2012年 | 13篇 |
2011年 | 11篇 |
2010年 | 9篇 |
2009年 | 7篇 |
2008年 | 7篇 |
2007年 | 5篇 |
2006年 | 7篇 |
2005年 | 2篇 |
2004年 | 5篇 |
2003年 | 2篇 |
2002年 | 2篇 |
2001年 | 1篇 |
2000年 | 3篇 |
1999年 | 2篇 |
1998年 | 1篇 |
1997年 | 3篇 |
1996年 | 1篇 |
1992年 | 2篇 |
1989年 | 1篇 |
排序方式: 共有165条查询结果,搜索用时 15 毫秒
1.
Identification of internal control genes in milk‐derived mammary epithelial cells during lactation cycle of Indian zebu cow 下载免费PDF全文
2.
Kai Wang Zhiqiang Zhu Huagang Huang Tingqiang Li Zhenli He Xiaoe Yang Ashok Alva 《Journal of Soils and Sediments》2012,12(4):556-564
Purpose
Soil contamination by multiple organic and inorganic contaminants is common but its remediation by hyperaccumulator plants is rarely reported. The growth of a cadmium (Cd) hyperaccumulator Sedum alfredii and removal of contaminants from Cd and polycyclic aromatic hydrocarbons (PAHs) co-contaminated soil were reported in this study. 相似文献3.
Lei Y Mulchandani P Chen W Mulchandani A 《Journal of agricultural and food chemistry》2005,53(3):524-527
A microbial biosensor for rapid, sensitive, selective, and cost-effective determination of the total content of organophosphorus nerve agents with p-nitrophenyl substituent is reported. The biosensor consisted of genetically engineered PNP-degrader Pseudomonas putida JS444 expressing organophosphorus hydrolase (OPH) on its cell surface immobilized on a dissolved oxygen electrode. Surface-expressed OPH catalyzed the hydrolysis of organophosphorus pesticides with p-nitrophenyl substituent such as paraoxon, methyl parathion, and parathion to release p-nitrophenol that was oxidized by the enzymatic machinery of Pseudomonas putida JS444 to carbon dioxide while consuming oxygen. The oxygen consumption was measured and correlated to the concentration of organophosphates. The sensor signal and response time were optimized with 0.086 mg dry weight of cell and operating in 50 mM pH 7.5 citrate-phosphate buffer with 50 microM CoCl(2) at room temperature. When operated at optimized conditions, the biosensor measured as low as 55 ppb of paraoxon, 53 ppb of methyl parathion, and 58 ppb of parathion without interference from most phenolic compounds and other commonly used pesticides, such as atrazine, coumaphos, sutan, sevin, and diazinon. The operational life of the microbial biosensor was approximately 5 days when stored in the operating buffer at 4 degrees C. 相似文献
4.
Parul Singh Anjil Kumar Srivastava Ashok Kumar Singh 《Pesticide biochemistry and physiology》2007,89(3):216-219
In this study, the comparison of cytogenetic effects of insecticide and fungicide in different phases of cell cycle was investigated in the root tip cells of barley (Hordeum vulgare L.). The seeds of H. vulgare L. Var. Karan 16 were treated with different concentrations (0.05%, 0.1% and 0.5%) of insecticide Profenophos (PF) and fungicide Mancozeb (MZ) for 6 h after presoaking durations of 7, 17 and 27 h.The different presoaking durations were used to bring the cells in various phases of cell cycle. Negative control was run parallel in distilled water. Cytogenetic examinations of root meristems exposed to the PF and MZ showed significant inhibition of mitotic index (MI) as well as significant increase in chromosomal aberrations (CAs). These parameters were dependent on the concentrations of insecticide and fungicide. The present study shows that PF and MZ both caused more damage in S phase of cell cycle which indicates that S phase is more sensitive in comparison to other phases. 相似文献
5.
6.
Gayacharan Tripathi Kuldeep Aski Muraleedhar S. Singh Neeta Kumar Ashok Lal Hanuman 《Genetic Resources and Crop Evolution》2022,69(3):1229-1245
Genetic Resources and Crop Evolution - Blackgram [Vigna mungo (L.) Hepper] is a protein rich pulse crop of Indian origin. The crop is cultivated in diverse range of agro-ecological regions,... 相似文献
7.
A. A.?WamanEmail author Pooja?Bohra B. N.?Sathyanarayana K.?Umesha G. K.?Mukunda T. H.?Ashok Balakrishna?Gowda 《Erwerbs-Obstbau》2015,57(3):153-164
Factors concerning aseptic culture establishment and hardening were studied in detail in choicest Silk Banana. Effect of size of sucker (small, medium and large), carbon concentration (10, 20 and 30 g/l), season of initiation (wet and dry) and pre-treatments such as segmentation and incision to the explants were studied during initiation. Further, hardening related factors such as substrates used for primary and secondary hardening, nursery nutrition (source and frequency of application) and pre-treatments for ex vitro rooting were also studied. Results revealed that small suckers were most suited for initiations with the least contamination, maximum establishment and higher percentage of greening. Lower concentration of sucrose, though delayed greening, resulted in 100?% establishment of explants. Initiations performed during the drier period were completely free from the fungal contamination and showed less bacterial contamination than those performed during the rainy season. Segmentation of explant into four parts during first subculture supported maximum shoot proliferation by overcoming apical dominance. Coir pith was observed to be the most congenial substrate during primary hardening, whereas coir pith alone or sand: red earth: coir pith (1:1:1) supported superior performance of plantlets during secondary hardening. Single application of mono ammonium phosphate improved growth of plantlets during secondary hardening. Pre-treatment with mono ammonium phosphate gave the best response in terms of rooting and hardening of un-rooted micro-shoots. Thus, the discussed methodology could help the industries to take up commercial scale propagation of Silk Banana. 相似文献
8.
Identification of a diverse mini‐core panel of Indian rice germplasm based on genotyping using microsatellite markers 下载免费PDF全文
Kapil K. Tiwari Anshuman Singh Sasmita Pattnaik Maninder Sandhu Sukhdeep Kaur Sourabh Jain Sushma Tiwari Shweta Mehrotra Mahender Anumalla Rashmita Samal Jyoti Bhardwaj Neha Dubey Vikrant Sahu Gayle A. Kharshing Patu K. Zeliang Kadiri Sreenivasan Pankaj Kumar Swarup K. Parida Sevanthi V. A. Mithra Vandana Rai Wricha Tyagi Pawan K. Agrawal Atmakuri R. Rao Arunava Pattanayak Girish Chandel Ashok K. Singh Ishwari S. Bisht Kangila V. Bhat Gundimeda J. N. Rao Jitendra P. Khurana Nagendra K. Singh Trilochan Mohapatra 《Plant Breeding》2015,134(2):164-171
Identification of a small core germplasm set representing the available genetic diversity is essential for its proper evaluation and subsequent utilization in rice improvement programmes. For constituting a small diverse mini‐core panel of Indian rice germplasm, a representative set of 6912 accessions drawn based on their geographic origin from the whole rice germplasm collection available in the National Gene Bank was genotyped using 36 microsatellite markers. Automated fragment analysis of amplicons yielded a total of 435 alleles, with an average 12.4 and range of 3–29 alleles per locus. Polymorphism information content (PIC) ranged from 0.08 (RGNMS190) to 0.86 (RM552) with an average of 0.528. Based on genotyping data, a mini‐core consisting of 98 genotypes was identified. Ninety‐four per cent of the alleles present in the core set were present in the mini‐core. The identified small but diverse panel will be useful for further intensive trait‐specific evaluation and utilization in allele mining. 相似文献
9.
The N loss from Vertisols was estimated by measuring the loss of 15N-labelled urea N under conditions that promote NH3 volatilization. Urea granules were placed on the top of 150-mm deep soil columns (Vertisols) collected from three sites with a range in pH, electrical conductivity, and cation exchange capacity. There were two contrasting moisture treatments, one near field capacity (wet) and another with intermittent wetting of the soil surface before allowing the columns to dry (moist-dry). The results indicated that losses were influenced markedly by pH and moisture treatment, being 29.5, 33.5, and 33% from the wet soils and 37, 42, and 40.5% from the moistdry soils with pH values of 7.7, 8.2, and 9.3, respectively. These observations clearly indicate that broadcasting of urea on the surface of Vertisols may cause substantial N losses. 相似文献
10.
Zhou D Cantu C Sagiv Y Schrantz N Kulkarni AB Qi X Mahuran DJ Morales CR Grabowski GA Benlagha K Savage P Bendelac A Teyton L 《Science (New York, N.Y.)》2004,303(5657):523-527
It is now established that CD1 molecules present lipid antigens to T cells, although it is not clear how the exchange of lipids between membrane compartments and the CD1 binding groove is assisted. We report that mice deficient in prosaposin, the precursor to a family of endosomal lipid transfer proteins (LTP), exhibit specific defects in CD1d-mediated antigen presentation and lack Valpha14 NKT cells. In vitro, saposins extracted monomeric lipids from membranes and from CD1, thereby promoting the loading as well as the editing of lipids on CD1. Transient complexes between CD1, lipid, and LTP suggested a "tug-of-war" model in which lipid exchange between CD1 and LTP is on the basis of their respective affinities for lipids. LTPs constitute a previously unknown link between lipid metabolism and immunity and are likely to exert a profound influence on the repertoire of self, tumor, and microbial lipid antigens. 相似文献