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121.
Tomoko Ikeda Keiichi Nagata Hiroshi Honda Toshio Shono Toshio Narahashi 《Pest management science》1998,52(4):337-342
Sikimi plant (also known as Japanese star anise), Illicium anisatium, is toxic to mammals. Extracts of Sikimi were studied for their insecticidal activity against the larvae of mosquito, Culex quinquefasciatus, and for their mechanism of action on ion channels. Crude methanol extract and its ethyl acetate-soluble fractions were insecticidally active, with EC50 values of 63·0 μg ml-1 and 43·7 μg ml-1, respectively. The ethyl acetate-soluble fraction was perfused through the bathing solution and the current induced by a brief (10 ms) application of GABA by pressure ejection through pipette electrode was recorded by the whole-cell patch clamp technique. The extract suppressed GABA-induced currents irreversibly with an EC50 value of 0·42 μg ml-1. The time constant of current fitted to the single exponential function was shortened by the ethyl acetate-soluble fraction at concentrations ranging from 0·1 μg ml-1 to 10 μg ml-1 in a concentration-dependent manner. It was concluded that Sikimi extracts decreased the affinity of GABA for its binding site on the GABA receptor, thereby suppressing GABA-induced currents. © 1998 SCI 相似文献
122.
Kelly Vargas Yoshiho Asakura Minoru Ikeda Nobuhiko Taniguchi Yasuhiro Obata Katsuyuki Hamasaki Kotaro Tsuchiya Shuichi Kitada 《Fisheries Science》2008,74(3):533-543
ABSTRACT: Allozyme variation of the littleneck clam Ruditapes philippinarum was evaluated in four samples from Nameishi and Matsuo in the Ariake Sea, Ryugatake and Ushibuka in the Shiranui Sea off Kyushu Island, Japan, and in one sample from Jinzhou, China, in the Bohai Sea. A Ruditapes bruguieri sample imported from the Korean Bay off Nampo, North Korea was also studied. Among the R. philippinarum samples, heterozygosity varied from 0.265 to 0.301 and F IS estimates indicated significant homozygosity excess in 15 of 40 loci analyzed. Deviations from Hardy–Weinberg equilibrium were significant in all samples ( P < 0.05). Pairwise F ST estimates indicate that genetic differences between the Chinese and Japanese samples were very low, but significantly different from zero. Mixture proportions with 95% confidence intervals of Chinese R. philippinarum in Nameishi and Matsuo were estimated at 0.4098 [0.2512, 0.5705] and 0.4899 [0.3262, 0.6540], respectively. However, genetic invasion of stocked Chinese R. philippinarum into wild populations in the Ariake Sea remains uncertain due to the low precision of the estimates caused by the high similarity of allele frequencies between Jinzhou and the Ariake Sea. 相似文献
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124.
Hiroyuki Ikeda Akihiko Kamoshita Junko Yamagishi Makara Ouk Bunna Lor 《Paddy and Water Environment》2008,6(1):91-103
In order to assess direct seeding of rice technology to cope with future agricultural labor shortage in Cambodia, agronomic
experiments were conducted in 2005 and 2006 to compare direct seeding with transplanting under three water conditions (non-flooded,
shallow flooded, and deep flooded conditions) with/without weed control by herbicides (bentazone and cyhalofop-butyl) for
two Cambodian rice varieties (shorter stature and early maturity Sen Pidao, taller stature and longer maturity Phka Rumduol).
Average rice yield in 2 years was lower in direct seeding (341 g m−2) than transplanting (404 g m−2), but interaction components with year, varieties, water conditions, and weed management were significant, and the attained
maximum yield of direct seeding (510 and 464 g m−2 for Phka Rumduol variety in shallow flooded condition with weeding in 2005 and 2006, respectively) was similar to that of
transplanting. Plant length and dry weight of rice were reduced in non-flooded and deep flooded conditions compared with shallow
flooded condition, and grain yield was the highest in shallow flooded condition. Yield advantage of Phka Rumduol over Sen
Pidao increased under direct seeding, particularly under non-flooded conditions in 2005 because weed infestation was more
suppressed in Phka Rumduol even without weeding. Increase in 100 g m−2 of weed infestation prior to heading (dry weight basis) reduced about 20% of attainable yield with weed control. This study
identified importance of stature and growth duration of rice varieties and presence of standing water as well as the weed
control, in order to develop and extend direct seeding in the Cambodia. 相似文献
125.
Asami Yoshida Inwoo Bae Hiroko Sonoda Ryuichi Masuo Sachie Oda Min-Jie Cao Kiyoshi Osatomi Kenji Hara 《Fisheries Science》2009,75(5):1317-1322
Gelatinolytic enzymes were partially purified from the skeletal muscle of red sea bream Pagrus major and characterized to obtain information on post mortem tenderization of fish muscle. Four gelatinolytic activities, G1 (90 kDa),
G2 (65 kDa), G3 (60 kDa), and G4 (100 kDa), were detected in the Q Sepharose column. G1, the major gelatinolytic enzyme, and
G4 were identified as serine proteinases from results of inhibitor spectrum and substrate specificity. By contrast, G2 and
G3 were found to be metalloproteinases since these were inhibited by ethylenediamine tetraacetic acid and o-phenanthroline, and activated by 4-aminophenylmercuric acetate. The optimum pH and temperature of these enzymes were in the
ranges of 7–9 and 20–40°C, respectively. 相似文献
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129.
Chuma T Ikeda T Maeda T Niwa H Okamoto K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(9):1027-1029
Cecal contents (16 samples/each flock) of broilers derived from 212 flocks were investigated for colonization of Campylobacter from 1995 to 1999 in the southern part of Japan, and the isolates were tested for antimicrobial susceptibilities. C. jejuni-positive flocks numbered 42 (19.8%) and C. coli-positive ones 26 (12.3%); Campylobacter spp. were recovered from 68 flocks (32.1%) in total. MICs of ampicillin, erythromycin (EM), tetracycline, nalidixic acid (NA), norfloxacin (NFLX), and ofloxacin (OFLX) to these 68 Campylobacter isolates were determined. Quinolone-resistant Campylobacter isolates numbered 22 (32.4%). All the isolates except one were cross-resistant to NA, OFLX, and NFLX. A high frequency of quinolone-resistance was found in both C. jejuni and C. coli, whereas a high level of EM-resistance was found in only C. coli strains. All C. jejuni isolates were sensitive to EM. 相似文献
130.
A captive-born 8-day-old male rainbow lorikeet (Trichoglossus haematodus) was found dead. Histologically, there were necrotizing hepatitis, myocarditis, and ventriculitis. Silver stain revealed argyrophilic filamentous bacilli within hepatocytes, smooth myofibers of the gizzard, and cardiac myofibers surrounding foci of necrosis. Immunohistochemistry using anti-Clostridium piliforme RT and MSK strain antisera reacted positively against bacilli within hepatocytes, cardiac myofibers, smooth myofibers of the gizzard, and splenic and intestinal macrophages. Polymerase chain reaction (PCR) assay of paraffin-embedded liver, heart, gizzard, spleen, and small intestine amplified the 196-bp DNA fragment specific to 16S ribosomal RNA of C. piliforme. The results of histopathology, immunohistochemistry, and PCR are consistent with C. piliforme infection in this lorikeet. 相似文献