Assessment of genetic diversity of Xanthomonas campestris pv. campestris isolates from Israel by various DNA fingerprinting techniques

Three molecular typing methods were used to investigate genetic diversity among Xanthomonas campestris pv. campestris isolates obtained in Israel and others previously obtained from different geographical locations (collection isolates). Using pulsed-field gel electrophoresis (PFGE), amplified fragment length polymorphism (AFLP) and repetitive sequence-based PCR (rep-PCR), 22 different isolates were divided into 11, 12 and 13 differentiated genotypes, respectively. All collection isolates yielded different genotypes and, among the isolates from Israel, several new genotypes were found. These findings not only support the observed heterogeneity within X. campestris pv. campestris , but also suggest that variability at the genomic level in this pathovar is higher than previously estimated. Moreover, while previous studies suggested that PCR patterns obtained with integron-specific primers are conserved in most X. campestris pathovars, PCR patterns of this element yielded four different types among the X. campestris pv. campestris isolates tested, thus supporting the relatively high diversity in this pathovar. Although differences in pathogenicity were observed among isolates, assays using cauliflower and radish did not indicate a correlation between pathogenicity and genotype.     

Cultivation of medicinal isabgol (Plantago ovata) in alkali soils in semiarid regions of Northern India

There is growing global demand for medicinal drugs including isabgol (Plantago ovata). With increasing demand of food for an ever‐increasing population in India, it is not possible to bring arable lands under cultivation for aromatic and medicinal plants. Salt‐affected lands (both saline and alkali) occupy about 8·6 million ha. Due to poor physical properties and excessive exchangeable Na⁺, most of these lands do not support good vegetation cover. The marginal and salt‐affected lands could be successfully utilized for the cultivation of aromatic and medicinal plants. We achieved almost complete germination of isabgol seeds using up to 5000 ppm salt‐solution. Grain yield (including husk) was 1·47 to 1·58 t ha⁻¹ at pH 9·2 showing no significant yield reduction as compared to normal soil. At pH 9·6 the grain yield was 1·03 to 1·12 t ha⁻¹. At higher pH there was significant reduction in yield. Sowing in good moisture (at field capacity) of soil was found best, but to save time sowing at shallow depth in dry soil, followed by irrigation was also suitable as compared to broadcasting seeds. The chlorophyll content was greater 70 days after sowing compared to younger stages (50 days after sowing). The total chlorophyll and plant biomass were lower from crops grown by broadcasting methods of sowing as compared to two other methods of sowing. The leaf area index (LAI) was higher for the broadcasting method of sowing as compared to the other two methods. Na⁺ absorption increased and K⁺ and K⁺/Na⁺ ratio decreased with increase in pH. Results reported in this paper clearly indicate that isabgol can successfully be grown on moderately alkali soils up to pH 9·6 without the application of any amendment. Copyright © 2006 John Wiley & Sons, Ltd.     

Biodrainage potential of <Emphasis Type="Italic">Eucalyptus tereticornis</Emphasis> for reclamation of shallow water table areas in north-west India

Ground water table (g.w.t.) levels were measured twice a month for 2 years in 50 observation wells installed inside and outside the two 18-year-old and 350 m apart plantations of Eucalyptus tereticornis (Mysure gum) at Dhob-Bhali research plot located in Rohtak district of Haryana state (north-west India). Throughout the study, the g.w.t. underneath the plantations remained lower than the g.w.t. in the adjacent fields. The average g.w.t. in the plantations was 4.95 m and the average g.w.t. in the control located in adjacent fields was 4.04 m. Interestingly, the spatial extent of lowering of g.w.t. in the adjacent fields was up to a distance of more than 730 m from the edge of a plantation. Drawdown in the g.w.t. developed due to the effect of a plantation was similar to the cone of depression of a pumping well and the drawdown in the g.w.t. developed due to the joint effect of both the plantations was similar to the combined cone of depression of two pumping wells. There was no correlation between soil salinity and the g.w.t. The fluctuations in g.w.t. caused fluctuations in g.w.t. salinity in the plantation as well as in the adjacent fields, but there was no net increase in g.w.t. salinity underneath the plantation. Sinker roots of Eucalyptus tree reached the zone of capillary fringe up to a depth of 4.40 m, indicating that the Eucalyptus trees were absorbing capillary water of the g.w.t. Thus, in shallow g.w.t. areas of semi-arid regions with alluvial sandy loam soils, the plantations of E. tereticornis act as bio-pumps and therefore, we recommend closely spaced parallel strip plantations of this species for the reclamation of waterlogged areas.     

Effect of feeding crude Red Palm Oil (Elaeis guineensis) and grain Amaranth (Amaranthus paniculatus) to hens on total lipids,cholesterol, PUFA levels and acceptability of eggs

Eggs, though a very nutritious food, also have high amounts of cholesterol and hence are not recommended to be consumed regularly by persons having hypercholesterolemia and associated cardiovascular diseases (CVD). In this context, an attempt was made in this study to reduce the cholesterol content of eggs by diet manipulation, using two naturally available and already proved hypocholesteromic agents [Red Palm Oil (RPO) and Grain Amaranth]. Thirteen experimental rations using raw and popped grain Amaranth and RPO were fed to 24 weeks old hens for a period of 6 weeks, singularly and in combinations. Total lipids, cholesterol and PUFA contents were analyzed in the experimental and control eggs. The results showed that RPO and RPO + Popped Amaranth feeding resulted in a maximum reduction in total lipids and cholesterol contents. Significant increase was observed in linoleic acid content in RPO + popped Amaranth; raw Amaranth and RPO fed groups. Acceptability studies showed that the products made from lower cholesterol eggs were well accepted.     

Molecular distinction and population structure of Indian mustard [Brassica juncea (L.) Czern.]

Genetic Resources and Crop Evolution - Rapeseed-mustard is one of the most important oilseed crops and providing a major source of edible oil in the world besides having other economic importance...