An Outbreak of Gangrenous Syndrome among Buffaloes and Cattle in West Bengal: Clinicopathological Studies

Gangrenous syndrome/Degnala disease was recorded in a large number of buffaloes and cattle in Murshidabad district of West Bengal, India. Fusarium spp. had been isolated from the mouldy paddy straw which were fed to the animals. There was a reduction in the incidence of the disease following withdrawal of the mouldy paddy straw. Histopathological examination showed necrosis and loss of architectural details in the skin.     

Subcutaneous pharmacokinetics and dosage regimen of cefotaxime in buffalo calves (Bubalus bubalis)

The pharmacokinetics and dosage regimen of cefotaxime following its single subcutaneous administration (10 mg/kg) were investigated in buffalo calves. Plasma and urine samples were collected over 10 and 24 h post administration, respectively. Cefotaxime in plasma and urine was estimated by microbiological assay technique using E. coli as test organism. The pharmacokinetic profiles fitted one-compartment open model. The peak plasma levels of cefotaxime were 6.48 ± 0.52 µg/ml at 30 min and the drug was detected upto 10 h. The absorption half-life and elimination half-life were 0.173 ± 0.033 h and 1.77 ± 0.02 h, respectively. The apparent volume of distribution and total body clearance were 1.17 ± 0.10 l/kg and 0.45 ± 0.03 l/kg/h, respectively. The urinary excretion of cefotaxime in 24 h, was 5.36 ± 1.19 percent of total administrated dose. A satisfactory subcutaneous dosage regimen for cefotaxime in buffalo calves would be 13 mg/kg repeated at 12 h intervals.     

Molecular systematics in Chrysanthemum × grandiflorum (Ramat.) Kitamura

An attempt was made to understand the molecular systematics and genetic differences between 10 original chrysanthemum cultivars and 11 mutants. The similarity among the cultivars and mutants varied from 0.17 to 0.90 using RAPD analyses, a simple but efficient method to distinguish cultivars and to assess parentage. Two distinct groups were found. Two cultivars were present as a separate group showing differences from all other cultivars. Mutants with different flower colour could be identified at the molecular level using RAPD technique holding promise to identify unique genes as SCAR markers. A high genetic distance among the different chrysanthemums showed that there exists a possibility of introgressing new and novel genes from the chrysanthemum gene pool.     

Genetic and physiological evidence for the production of N-acyl homoserine lactones by Pseudomonas syringae pv. syringae and other fluorescent plant pathogenic Pseudomonas species

N-acyl homoserine lactones (AHLs) function as cell density (quorum) sensing signals and regulate diverse metabolic processes in several gram negative bacteria. We report that strains of Pseudomonas syringae pvs. syringae (Pss), tabaci and tomato as well as P. corrugata and P. savastanoi produce difussible AHLs that activate the lux operons of Vibrio fischeri or the tra::lacZ fusion of Agrobacterium tumefaciens. In Pss strain B3A, AHL production occurs in cell density dependent manner. Nucleotide sequence and genetic complementation data revealed the presence of ahlI_Pss, a luxI homolog within the Ahl⁺ DNA of Pss strain B3A. The DNA expresses in AHL-deficient strains of P. fluorescens and E. carotovora subsp. carotovora (Ecc), and restores extracellular enzyme production and pathogenicity in the Ecc strain. The derivatives of Pss strains B3A and 301D carrying chromosomal ahlI::lacZ do not produce AHL, but like their wild type parents, produce extracellular protease and the phytotoxin syringomycin as well as elicit the hypersensitive reaction in tobacco leaves. While these strains also produce a basal level of -galactosidase activity, the expression of ahlI::lacZ is substantially stimulated in the presence of multiple copies of the DNA or by the addition of cell-free spent cultures containing AHL. The activation of -galactosidase production occurs with spent cultures of some, but not all Pseudomonas strains which produce AHL as indicated by the Lux and tra::lacZ assays. Pss strains deficient in the global regulatory genes, gacA or lemA, produce very low levels of AHL. Since inactivation of ahlI_Pss eliminates AHL production and since Ahl⁺ Pseudomonas strains carry the homolog of ahlI_Pss, we conclude that ahlI_Pss specifies a key step in AHL biosynthesis and it has been conserved in many plant pathogenic pseudomonads.     

Establishment of Prosopis cineraria (L.) druce in the hot deserts of India

Prosopis cineraria (L.) Druce, commonly known as Jandi, is a deep rooted, nitrogen fixing, multipurpose tree endemic to the hot deserts of India. These trees are the essential component of the agroforestry land use system in these parts of India. The shade-intolerant tree reproduces poorly in nature and is difficult to propagate vegetatively. Germination up to 86.6% was observed after 15 days in seeds soaked in water at room temperature for 72 h. In another treatment, pouring of boiling water twice (at 0 and 6 h) over seeds and germinating after 12 h produced germination rates of 92.6%. Field establishment of containerized transplants in polythene bags (22×10 cm; 150 gauge) gave 90.3% survival after 6 months and 75.4% survival after 24 months. Seedlings were irrigated once during transplanting with 15 1 water. Bare root transplants and manual direct seeding after 6 months had a survival of 30.8 and 45.0%, respectively. Increased levels of nutrients (N, P and K), moisture content and organic carbon were observed under plantations as compared to open areas.     

Effect of Growth Regulators on the Productivity of Some Major Oilseed Crops

A number of field experiments were conducted at the Viswavidyalaya Research Farm during 1984–85 to 1986–87 to study the effects of five growth regulators viz. Miraculan (a triacontanol based growth stimulant), Nutron (1-triacontanol growth stimulant), Planofix ( α -napthylacetic acid), Paras or Mixtalol (Higher alcoholic carbon compounds) and N-triacontanol on productivity of five different major oilseed crops viz. rapeseed ( Brassica campestris L. var. yellow sarson), mustard ( Brassica juncea L. Czern and Coss), sesame ( Sesamum indicum L.), linseed ( Linum usitatissimum L.) and safflower ( Carthemus tinctorius L.). Results showed the positive influence of growth regulators on the productivity of crops (10–40 % increase in yield); however the influence was not consistent over the years. N-triacontanol when tried on mustard only improved the grain yield significantly, more so when applied with paras or planofix, sesame was most influenced by spraying planofix and paras. Nutron favourably influenced safflower and linseed. The test weight of grains followed by the number of pods/plant were influenced most by the application of growth regulators. Inconsistency in results over the years, however, need further investigations on the timing of spraying.     

Genotypic variability in vegetable amaranth (Amaranthus tricolor L for foliage yield and its contributing traits over successive cuttings and years

Summary Twenty nine strains of vegetable amaranth (Amaranthus tricolor were grown for two successive seasons to study different selection parameters for foliage yield and its nine contributing morphological and quality traits. The strains AV-38 (5.06 kg/plot) and AV-31 (5.04 kg/plot) recorded highest foliage yield, followed by AV-30 (4.78 kg/plot) and AV-23 (4.70 kg/plot). The protein and carotenoid content averaged 1.24 ± 0.03 mg/100 mg and 0.83 ± 0.02 mg/g respectively. The leaves of A. tricolor also have considerable quantities of ascorbic acid (112.33 ± 5.00 mg/100 g) and fibre (8.39 ± 0.10%). The mean of individual cuttings for plant height, leaf size, stem diameter, foliage yield, protein, ascorbic acid and fibre content increased with successive cuttings till third cutting and thereafter showed a decline. Genotypic coefficient of variation (GCV) values ranged from 6.80 to 28.25%. However, the fibre content, branches/plant, leaves/plant, plant height and stem diameter showed lowest values of GCV. The values of heritability estimates were high for all the traits in all the cuttings as well as on pooled basis and ranged from 0.89 for branches/plant to 0.98 for foliage yield. Highest expected genetic advance was noticed for ascorbic acid (57.48%), followed by foliage yield (48.30%) and leaf size (29.51%).     

Is air-drying of soil samples an appropriate step in determining plant available potassium for corn?

Potassium (K) fertilizer recommendations are mainly based on air -dried soil samples which can lead to over- or under-estimation of plant available soil K. Three on-farm trials were conducted in North Dakota and Minnesota to determine the variation of soil test-K between air-dried (K_Dry) and field moist (K_Moist) soil samples. The differences between K_Dry and K_Moist decreased exponentially as soil K increased, but increased linearly with increasing soil moisture. Soil drying influenced the plant available soil K-test value, producing higher K values compared to the moist soil K. It is unclear based on these initial experiments which method might produce a more predictable K critical value to aid in directing K application for corn in this region.     

Thin layer chromatographic identification and quantitation of niacin and niacinamide in pharmaceutical preparations

A safe and simple thin layer chromatographic method has been developed for the estimation of niacin and niacinamide in pharmaceutical preparations containing other vitamins, enzymes, herbs, antiamoebic drugs, etc. The method involves removal of excipients from the preparation by ethanol precipitation and isolation of niacin or niacinamide from other ingredients by TLC on silica gel with water as developing solvent, followed by extraction in 0.1N HCl, and spectrophotometric estimation of the vitamin at 262 nm. The percent recoveries for niacin and niacinamide were 100.1 +/- 1.9 and 100.2 +/- 1.5, respectively.     

Increase in the Asian southwest monsoon during the past four centuries

Climate reconstructions reveal unprecedented warming in the past century; however, little is known about trends in aspects such as the monsoon. We reconstructed the monsoon winds for the past 1000 years using fossil Globigerina bulloides abundance in box cores from the Arabian Sea and found that monsoon wind strength increased during the past four centuries as the Northern Hemisphere warmed. We infer that the observed link between Eurasian snow cover and the southwest monsoon persists on a centennial scale. Alternatively, the forcing implicated in the warming trend (volcanic aerosols, solar output, and greenhouse gases) may directly affect the monsoon. Either interpretation is consistent with the hypothesis that the southwest monsoon strength will increase during the coming century as greenhouse gas concentrations continue to rise and northern latitudes continue to warm.