Evaluation of microbial indicators for the determination of bacterial groundwater contamination sources

Several different Microbial source tracking methods (MSTs) can be used to distinguish human from animal fecal contamination in water; In this study, experiments were carried out to test the effectiveness and reliability of three bacteria based approaches (the fecal coliforms to fecal streptococci ratio, antibiotic-resistant Clostridium perfringens, and human bifidobacteria) in a simulated groundwater micro-environment. The methods were evaluated in three phases: initially, the specificity of each indicator was validated on groundwater samples affected by known pollution source; then the variation of performance with time of each method was determined, and finally, the die-off coefficients for pure species of Clostridium perfringens and Bifidobacterium adolescentis were measured. The results indicate that only the determination of human bifidobacteria concentration can be considered reliable in distinguishing human from animal pollution in groundwater at the conditions applied. Nevertheless, human bifidobacteria were detectable only for two weeks after the contamination event. This study also shows that antibiotic resistant Clostridium perfringens detected using the Shahidi-Ferguson medium is not source specific, whereas it confirms that this species can be useful for timing general fecal contamination events.     

Soil properties and crop yields on a vertisol in India with application of distillery effluent

Distillery effluent, a foul smelling, dark coloured by-product of distillery industries, is usually applied as irrigation water or as an amendment to arable land in some areas which are in the vicinity of the distillery industries. A field experiment on soybean–wheat system was conducted for 3 consecutive years in a Vertisol of central India to evaluate the effect of distillery effluent (DE) as an amendment on soil properties and crop productivity. The treatments were control (no fertilizer or manure or DE, T₁), 100% NPK + FYM @ 4 Mg ha⁻¹ to soybean (T₂) and four graded levels of DE, viz.: 2.5 cm DE to soybean and wheat on residual nutrition (T₃), 2.5 cm DE to soybean and 1.25 cm to wheat (T₄), 5 cm DE to soybean and wheat on residual nutrition (T₅), 5 cm DE to soybean and 2.5 cm to wheat (T₆). The organic carbon, microbial biomass carbon and electrical conductivity (EC) of the surface (0–10 cm) soil increased significantly with application of DE compared to T₁ and T₂, but the soil pH was not affected. The EC increased from 0.47 dS m⁻¹ and 0.58 dS m⁻¹, respectively, in T₁ and T₂ to 1.52 dS m⁻¹ in T₆, where highest dose of DE was applied. This indicated a slight build-up of salinity with DE application. The application of DE showed a significant improvement in the physical properties of the soil. The mean weight diameter (MWD), saturated hydraulic conductivity, water retention at field capacity and available water content were significantly (P < 0.05) higher, while bulk density (BD) and penetration resistance of the surface soil were significantly lower (P < 0.05) in all DE treated plots except in T₃ than those in T₁ and T₂. The fractions of WSA of more than 1 mm diameter in T₆, T₅ and T₄ were, respectively, 141%, 107% and 116% more than the control. The MWD showed a positive linear relationship with the organic carbon (r = 0.84^**) and microbial biomass carbon (r = 0.90^**) of the soil. A significant (P < 0.01) negative linear relationship (r = 0.70^**) was found between soil organic carbon and BD. Except T₃, all the DE treated plots recorded significantly higher total and microporosity of the soil than control. Water retention at permanent wilting point and macroporosity of the soil were not affected by treatment. The seed yield of soybean in all the DE treatments was similar with T₂ (1.86 Mg ha⁻¹) but significantly more than control (1.28 Mg ha⁻¹). The DE application levels have not affected the seed yield of soybean. In wheat highest grain yield was recorded in T₂ (3.47 Mg ha⁻¹), which was similar with T₄ (3.16 Mg ha⁻¹), T₅ (3.22 Mg ha⁻¹) and T₆ (3.46 Mg ha⁻¹). DE application up to T₄ level was found suitable from productivity, salinity and sustainability point of view. The study showed that judicious application of DE as an amendment to the agricultural field could be considered as a viable option for safe disposal of this industrial waste.     

Prediction of the movement of perchlor0ethylene in soil columns

Many toxic volatile organic compounds (VOCs) are being released to the soil through improper waste disposal practices, industrial impoundments, landfills and accidental spills during transport and handling. Once released, the VOCs migrate toward the groundwater and cause contamination. One of the most frequently found VOCs in groundwater supplies is perchloroethylene (PCE). In this study an attempt was made to investigate the transport of PCE in two types of soil. PCE spills were created on soil columns in the laboratory and column breakthrough theory was used to analyze the data. Empirical equations were used to calibrate a contaminant transport model. The programs for this simple model have been prepared for use with IBM microcomputers and are capable to predicting the breakthrough time and concentration of PCE at various depths in the profile.     

Field-level comparison of nitrogen rates and application methods on maize yield,grain quality and nitrogen use efficiency in a humid environment

Appropriate nitrogen (N) management practices are of critical importance in improving N use efficiency (NUE), maize (Zea mays) yield and environmental quality. A six-year (2005–2010) on-farm trial was conducted in Ottawa, Canada to assess the effects of N rates and application methods on grain yield and NUE. In four out of the six-year study, grain yield increased by 60–77 kg ha^?1 by sidedress, compared to 49–66 kg ha^?1 for each kg N ha^?1 applied at preplant. Grain yield response to N between the two strategies was similar in the other growing seasons. Sidedress strategy required 15 kg N ha^?1 less of the maximum economic rate of N (MERN) than preplant application. Our results indicate that sidedress application of 90–120 kg N ha^?1 with a starter of 30 kg N ha^?1 resulted in greater yield, grain quality and NUE than preplant N application in this cool, humid and short growing-season region.     

Yield and water use efficiency of cauliflower under varying irrigation frequencies and water application methods in Lower Gangetic Plain of India

A field study (1999-2000 to 2001-2002) was carried out to optimize the irrigation frequency and suitable water application methods for cauliflower with a view to increase curd yield (CY) and water use efficiency (WUE). Check Basin (CB), Each Furrow (EF) and Alternate Furrow (AF) methods were tested with three irrigation frequencies depending on the attainment of soil matric potential (Ψ_m) value at 0.2 m depth as: −0.03 MPa (F₁), −0.05 MPa (F₂) and −0.07 MPa (F₃). Maximum CY was recorded under F₁ and decreased by 10.4 and 31.4%, respectively under F₂ and F₃ frequencies. In contrast, WUE decreased by 9.3% from F₃ to F₁. Highest CY and WUE obtained under CB followed by EF and AF methods. Furrow application methods saved 12-24% irrigation water over CB method. Maximum soil water stress coefficient (K_s) recorded at curd development stage in comparison to other stages. Both seasonal evapotranspiration (ET_a) and yield-moisture stress index (Kys) recorded positive linear relationships with CY. Present study shows a crop response factor of 0.822 for cauliflower. In this region, cauliflower should be irrigated with check basin method at an interval of 8-10 days.     

Sulfide Production by Sulfate Reducing Bacteria with Lactate as Feed in an Upflow Anaerobic Fixed Film Reactor

The sulfate reducing bacteria (SRB) have the capability of reducing sulfate (SO4-2) under anaerobic conditions into sulfide (S-2) which can precipitate metals as metal sulfides. The optimum conditions for sulfide production by SRB utilizing lactate, in an upflow anaerobic fixed film reactor (UAFFR) were not previously established. The main objective of this research was to investigate these conditions for the growth of SRB to ensure the highest sulfide production under consistent behaviour of the system. Substrate containing lactate as the organic carbon source along with sulfate, nitrogen and phosphorus as the required nutrients was used as a feed to the UAFER which was seeded with SRB. It was found that an optimum sulfide production occurred with an organic loading rate (OLR) of 6 kg d-1 m-3, while the theoretical oxygen demand to sulfate ratio (ThOD/SO4) ranged from 1.5 to 2.25. Also, the optimum total nitrogen and phosphorus demands were determined to be about 250 and 50 mg L-1 respectively. A total nitrogen concentration above 600 mg L-1 started showing toxicity and lowered the sulfide production. The optimum ThOD:N:P for sulfide production and growth of SRB in the UAFFR was 100:5:1 under optimum conditions.     

Radar detection of cloud-seeding effects

The effects on precipitation of artificially seeding clouds with Dry Ice have been monitored from cloud to ground with a radar that has a wavelength of 8.6 millimeters.     

Effect of feeding frequency on growth, survival and feed utilization in fingerlings of Catla catla (Hamilton), Labeo rohita (Hamilton) and Cirrhinus mrigala (Hamilton) in outdoor rearing systems

Fingerling rearing of Indian major carps, Catla catla, Labeo rohita and Cirrhinus mrigala, were conducted in concrete tanks under a polyculture system at a density of 0.3 million fry ha^?1 to evaluate the effect of one, two or three feedings per day on growth, survival and feed utilization. Fry were fed with formulated supplementary diet at 10% of biomass per day during the first 15 days, followed by 8% in the next 15 days and 6% thereafter, for 60 days. Feeding of fish three times daily led to higher growth (103.9±8.5 mm/10.3±2.4 g) than those feeding twice (100.2±4.8 mm/9.9±1.5 g) or once (97.2±9.8 mm/9.4±2.1 g) daily, although the values were not significantly different (P>0.05). Survival rate ranging between 72.3% and 75.1% also did not vary significantly (P>0.05) among the treatments. However, higher feeding frequencies resulted in better feed utilization as evident from decreasing feed conversion ratio values. The present study suggested requirement of a higher feeding frequency for rohu compared with catla and mrigal.     

Fishing and trade of devil rays (Mobula spp.) in the Bay of Bengal,Bangladesh: Insights from fishers’ knowledge

1. Devil rays (Mobula spp.) are globally threatened cartilaginous fishes that have attracted global conservation concern owing to their high extinction risk and lack of protection in many countries. Limited resources and data on threatened marine species, including devil rays, impede conservation actions, particularly in developing countries, many of which have high biodiversity.
2. Devil ray catch is a component of artisanal fisheries in Bangladesh, but data on their fisheries and trade are limited. To characterize devil ray fishing practices, fishers’ perception and trade, 230 fishers and traders were interviewed between 4 June 2018 and 22 June 2019, in four areas of south-east Bangladesh. Catch data were also opportunistically collected at landing sites.
3. Six devil ray species were documented, caught in an array of gill nets, set-bag nets and longlines. All interviewed fishers reported life-long devil ray bycatch in some numbers, and also noted a decline in catch over the last decade. Bottom trawling, increased bycatch levels, increased demand for devil ray products and, in some cases, ecosystem changes were identified by fishers as threats to devil ray populations.
4. Unregulated and undocumented trade and retained bycatch, especially by gill nets and set-bag nets, are fuelled by local consumption of devil ray meat and international trade in meat and gill rakers. Compliance with international trade control treaties for all Mobula spp. or the Bangladeshi law protecting Mobula mobular was low, with the majority of fishers (87%, n = 174) unaware of their existence.
5. To manage devil ray fisheries, and prevent possible population declines, we propose a combination of legally enforced gear modifications, and catch and trade control through community-owned implementation strategies. Additionally, we propose the simultaneous implementation of inclusive, community-based awareness and stewardship projects in conjunction with a coast-wide ray monitoring programme. Finally, we emphasize that more research and action rooted in a sustainable fishery model is urgently needed to protect Bangladeshi devil ray populations.