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191.

Objective

To evaluate the effect of hyaluronidase on uptake, duration and speed of elimination of xylazine–tiletamine–zolazepam administered in the subcutaneous fat over the dorsal lumbar region of swine.

Study design

Blinded, randomized, crossover study.

Animals

Six healthy Landrace/Large White pigs weighing 132 ± 24 kg (mean ± standard deviation).

Methods

Animals were administered xylazine (1 mg kg?1) and tiletamine–zolazepam (8 mg kg?1) (control treatment, CON), or xylazine–tiletamine–zolazepam at the same doses with hyaluronidase (400 IU) (treatment HYA). The treatments were administered into the dorsal lumbar adipose tissue, 2.5–3.0 cm laterally from the spinous process of the second lumbar vertebra. The latency, anesthesia and recovery periods were measured. Heart rate, noninvasive systolic, diastolic, and mean arterial pressures, respiratory rate, hemoglobin oxygen saturation and rectal temperature were recorded every 10 minutes for up to 50 minutes.

Results

One animal in CON and one animal in HYA were responsive to stimulation and did not allow safe handling. No significant difference was found between treatments for latency (CON 11.3 ± 5.9 minutes, HYA 7.4 ± 5.1 minutes) and anesthesia (CON 53 ± 53 minutes, HYA 49 ± 38 minutes) periods. Recovery period was shorter in HYA (9 ± 6 minutes) than in CON (32 ± 16 minutes) (p < 0.05). Physiological variables were not significantly changed over time and were within accepted normal clinical limits for the species in both treatments.

Conclusion and clinical relevance

Hyaluronidase (400 IU) administered into adipose tissue in pigs did not reduce the latency and duration of dissociative anesthesia, but was associated with faster recovery.  相似文献   
192.
The role of different factors such as biological material (tissues, organs) and trophic condition (overfeeding or not) in the metal accumulation was studied in three genotypes of ducks (Pekin, Muscovy, and Mule) under breeding conditions. Results showed that overfeeding decreased the concentration in Cd, Cu, and Zn through the dilution process. In contrast, mercury concentration increased with this method. A relation between lipidic metabolism of genotypes and the distribution of this metal in biological material was found. Domestic ducks were little contaminated, but a low chronic contamination in Cd was observed, probably coming from the food. Due to the low levels of contamination observed in these breeding ducks, they can be considered as a good control for further contamination studies and comparison with accumulation levels recorded in the field. The impact of feeding condition on accumulation showed the importance of taking into account the life cycle of birds before studying their contamination and the impact of pollutants.  相似文献   
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Consumers demand organic products because they believe they are more flavorful and respectful to the environment and human health. The effects of conventional, integrated, and organic farming, grown in a controlled greenhouse, on color, minerals, and carotenoids of sweet pepper fruits ( Capsicum annuum), cv. Almuden, were studied. Experimental results proved that organic farming provided peppers with the highest (a) intensities of red and yellow colors, (b) contents of minerals, and (c) total carotenoids. Integrated fruits presented intermediate values of the quality parameters under study, and conventional fruits were those with the lowest values of minerals, carotenoids, and color intensity. As an example, the concentrations of total carotenoids were 3231, 2493, and 1829 mg kg (-1) for organic, integrated, and conventional sweet peppers, respectively. Finally, organic red peppers could be considered as those having the highest antioxidant activity of all studied peppers (agricultural farming and development stage).  相似文献   
195.
Epidemiological data, clinical findings, laboratory data, medical imaging, and outcomes were reviewed in 29 dairy calves with otitis media/interna. Age at admission ranged from 1 to 24 wk. The majority of calves were referred during winter. Clinical signs included drooping ear, ptosis, head tilt, abnormal nystagmus, strabismus, dysphagia, regurgitation, stiff neck, opisthotonos, facial hyperesthesia, and purulent aural discharge. Intranasal endoscopic examination of 5 animals revealed nasopharyngeal collapse in 4. Cerebrospinal fluid (CSF) was abnormal in all of 7 cases. Mycoplasma bovis was cultured from all but 1 case with external ear or tympanic bullae samples (n = 12), and Mycoplasma arginini was cultured from the remaining ear sample. Radiographs of the tympanic bullae were performed in 24 calves, tomodensitometry (CT) in 3 calves and ultrasound in 4 calves. According to medical imaging techniques or necropsy, 69% of the cases were classified as chronic. Mean duration of treatment was 23.3 d. The rate of clinical recovery was 75%.  相似文献   
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199.
The resistance to Fusarium oxysporum f.sp. melonis (Fom) race 1.2 has been studied in melons, such as the Portuguese accession ‘BG-5384’ and in the Japanese ‘Shiro Uri Okayama’, ‘Kogane Nashi Makuwa’, and ‘C-211’, since a good characterization of the resistance is necessary before its introgression into commercial varieties. These four melon accessions showed a high level of resistance to races 0, 1, and 2 of Fom, indicating that the partial resistance to the race 1.2 previously detected may not have been race specific. To determine the mode of inheritance of the resistance to Fom race 1.2, the F1, F2, BCPR, and BCPS generations from the crosses between the four resistant accessions above and ‘Piel de Sapo’, a Fom race 1.2 susceptible melon, were developed. They were subsequently inoculated with two Fom isolates, one from the pathotype 1.2Y and the other from the pathotype 1.2W. The area under the disease progress curve was determined for each inoculated plant, and the data were analyzed. We show that the resistance seen in these accessions is polygenically inherited with a complex genetic control because many epistatic interactions were detected. The three epistatic effects; additivity × additivity, dominance × dominance, and dominance × additivity are present and significant, with differing magnitudes from one cross to the next. The relatively low heritabilities, and these epistatic effects make difficult the improvement of the resistance, from these sources, through a standard selection procedure.  相似文献   
200.
Twenty-six 6-week old female New Zealand White rabbits were divided into two groups: ad libitum (AL) and 70% restricted (RS) feeding. At the beginning of the experiment the liveweights were practically the same: 0.99 +/- 0.08 vs. 1.01 +/- 0.08 kg in group AL and RS, respectively. At 18 weeks of age the body weight of Group RS rabbits was 84.7% (3.14 +/- 0.24 kg) of the group AL (3.71 +/- 0.31 kg). The apparent digestibility of crude protein was significantly (p < 0.001) higher in Group RS than in Group AL (76.5 +/- 1.4 vs. 73.0 +/- 2.7%). The daily water consumption was significantly (p < 0.05) higher (3.5 ml/g DM intake) in Group RS as compared to Group AL (1.9 ml/g DM). Since the average body weight in Group RS at 18 weeks was the same as that of Group AL at 14 weeks of age (3.14 kg), the comparison of the live body measures and indices was based on these ages. Animals fed ad libitum or restricted show no differences at the defined age in most live body measurements except in heart girth and rump width, which were significantly (p < 0.05) shorter in Group AL than in Group RS (29.3 +/- 0.8, 5.7 +/- 0.5 and 30.7 +/- 1.0, 6.2 +/- 0.3 cm, respectively). Body capacity was significantly (p < 0.05) smaller in Group AL, as the head capacity-body capacity proportion was significantly (p < 0.05) higher in Group AL than in Group RS (1653.1 +/- 134.5, 22.0 +/- 2.5 and 1913.7 +/- 168.7, 17.7 +/- 1.9, respectively). Due to restricted feeding the growth of the head proved to be less intensive than that of the body at the same body weight. The body in these does tended to be wider. Since the head in comparison to length or capacity of the body was smaller in does fed 70% of ad libitum, it could be concluded that the development of body parts of restricted-fed does was unequal (allometric growth). The effect of feed restriction reflected in lower dry matter and fat, and a higher ash and protein content both in total body and in dry matter composition of rabbits at 18 weeks of age. Restricted feeding delayed sexual maturity (69 vs. 92% of rabbits) with later starting ovarian activity, weaker ovarian responsiveness, and a smaller number of tertiary follicles on the ovarian surface.  相似文献   
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