Multisystemic bovine mycobacteriosis in a pony with neurological signs and weight loss

This report describes the clinical, biochemical and histopathological findings of a case of disseminated, primary mycobacterial infection with Mycobacterium bovis in a pony presenting with neurological signs and weight loss. This report revisits tuberculosis as a potential differential diagnosis in horses at a time of relatively strong tuberculosis control in the UK and iterates the importance of post‐mortem examination as a diagnostic tool for clinicians. Key public health questions following exposure to mycobacterial pathogens are also discussed.     

植酸酶对不同类型的断乳仔猪日粮粗蛋白、氨基酸和能量的消化率的影响

通过对4个安装了简易T型瘘管的断奶仔猪研究表明,添加植酸酶并没有提高玉米-豆粕型日粮(试验组1)、小麦-豆粕型日粮(试验组2)和大麦-豌豆-菜籽粕型日粮(试验组4)粗蛋白和氨基酸的回肠表观消化率(AID),而使小麦-豆粕-菜粕型日粮的粗蛋白和氨基酸回肠表观消化率或粗蛋白和消化能的总消化道表观消化率(ATTD)得到了显著的提高(P<0.05)或呈提高的势态(P<0.10)。这些结果表明,氨基酸对细菌性植酸酶的反应因子取决于日粮的组成。     

Natural Polyether Ionophores and Their Pharmacological Profile

This review is devoted to the study of the biological activity of polyether ionophores produced by bacteria, unicellular marine algae, red seaweeds, marine sponges, and coelenterates. Biological activities have been studied experimentally in various laboratories, as well as data obtained using QSAR (Quantitative Structure–Activity Relationships) algorithms. According to the data obtained, it was shown that polyether toxins exhibit strong antibacterial, antimicrobial, antifungal, antitumor, and other activities. Along with this, it was found that natural polyether ionophores exhibit such properties as antiparasitic, antiprotozoal, cytostatic, anti-mycoplasmal, and antieczema activities. In addition, polyethers have been found to be potential regulators of lipid metabolism or inhibitors of DNA synthesis. Further study of the mechanisms of action and the search for new polyether ionophores and their derivatives may provide more effective therapeutic natural polyether ionophores for the treatment of cancer and other diseases. For some polyether ionophores, 3D graphs are presented, which demonstrate the predicted and calculated activities. The data presented in this review will be of interest to pharmacologists, chemists, practical medicine, and the pharmaceutical industry.     

Metabolic Profiling and In Vitro Assessment of the Biological Activities of the Ethyl Acetate Extract of Penicillium chrysogenum “Endozoic of Cliona sp. Marine Sponge” from the Red Sea (Egypt)

Marine sponge-derived endozoic fungi have been gaining increasing importance as promising sources of numerous and unique bioactive compounds. This study investigates the phytochemical profile and biological activities of the ethyl acetate extract of Penicillium chrysogenum derived from Cliona sp. sponge. Thirty-six compounds were tentatively identified from P. chrysogenum ethyl acetate extract along with the kojic acid (KA) isolation. The UPLC-ESI-MS/MS positive ionization mode was used to analyze and identify the extract constituents while 1D and 2D NMR spectroscopy were used for kojic acid (KA) structure confirmation. The antimicrobial, antioxidant, and cytotoxic activities were assessed in vitro. Both the extract and kojic acid showed potent antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa with MIC 250 ± 0.82 µg/mL. Interestingly, the extract showed strong antifungal activity against Candida albicans and Cryptococcus neoformans with MIC 93.75 ± 0.55 and 19.53 ± 0.48 µg/mL, respectively. Furthermore, KA showed the same potency against Fusarium oxysporum and Cryptococcus neoformans with MIC 39.06 ± 0.85 and 39.06 ± 0.98 µg/mL, respectively. Ultimately, KA showed strong antioxidant activity with IC₅₀ 33.7 ± 0.8 µg/mL. Moreover, the extract and KA showed strong cytotoxic activity against colon carcinoma (with IC₅₀ 22.6 ± 0.8 and 23.4 ± 1.4 µg/mL, respectively) and human larynx carcinoma (with equal IC₅₀ 30.8 ± 1.3 and ± 2.1 µg/mL, respectively), respectively. The current study represents the first insights into the phytochemical profile and biological properties of P. chrysoenum ethyl acetate extract, which could be a promising source of valuable secondary metabolites with potent biological potentials.     

Babesia vulpes in a dog from Prince Edward Island,Canada

A 12-year-old neutered male American Staffordshire terrier dog was referred to the Atlantic Veterinary College, Prince Edward Island, Canada, for suspected immune-mediated hemolytic anemia. Babesiosis (Babesia vulpes) was confirmed using polymerase chain reaction testing. The dog was successfully treated with a 10-day protocol of atovaquone/proguanil (TEVA Pharmaceutical Industries, Toronto, Ontario), 13.5 mg/kg BW, PO, q8h and azithromycin (Pharmascience, Montreal, Quebec), 10 mg/kg BW, PO, q24h. To the authors’ knowledge, this report is the first documented case of babesiosis caused by Babesia vulpes in a dog from Canada.     

Cardiovascular effects of intermittent or continuous treadmill conditioning in horses

Nine mature Quarter Horse mares were trained for ten weeks by continuous (n=4) or intermittent (n=5) treadmill work. Both groups of horses trotted at 3.3m/sec. The continuous work was performed at a 3% incline while the intermittent work was done at a 9% incline. Heart rate, cardiac output and blood lactate concentration were measured during a standard exercise test that included work on both treadmill grades before and after five and ten weeks of training. The two conditioning programs did not produce differences in the measured parameters of the two groups of horses. However, there was an overall conditioning effect observed in both groups of horses throughout the ten weeks of treadmill conditioning. Heart rate decreased (P<.05) during exercise on both treadmill grades after ten weeks of training. Lactate concentration decreased (P<.05) during the 9 percent grade trot after five weeks of training. Only slight further decreases occurred between week five and week ten of conditioning. Cardiac output increased (P<.05) between rest and exercise on both grades but there was no conditioning effect.