Effect of heavy metals on growth and survival of Macrophomina phaseolina (Tassi) Goid.

Summary The effects of Cd, Co, Ni and Zn on growth and survival of Macrophomina phaseolina were studied in vitro. Cd, Ni and Co at 500 g ml^–1 inhibited growth by 78.8%, 73.6% and 11.8%, respectively, after 4 days at 25 ± 1°C. The mycelial dry weight yield was enhanced by 2.1% at 100 g ml^–1 Zn. The population of the pathogen declined in soil amended separately with Cd, Co, Ni and Zn. Cd (4000 mg kg^–1) proved to be the most toxic, by completely inhibiting the survival of the pathogen in soil after 20 days.     

Effects of solar treatment on germination of sclerotia (Sclerotium rolfsii Sacc.) and on other soil mycoflora

Summary Only 10% of sclerotia germinated in a tarpcovered plot after 45 days of treatment while 50% of sclerotia germinated in the control plot. However, complete inhibition of sclerotia germination was not achieved. The maximum temperature recorded was 53°C, after 45 days of treatment in a tarped plot at 1 cm depth. The incidence of Acrophialophora fusispora, Aspergillus spp., Spicaria sp., and Trichoderma viride increased in soil after 45 days of the solar treatment.     

Genetics of nonnodulation in groundnut (Arachis hypogaea L.). Studies with single and mixed Rhizobium strains

Summary Genetic studies of nonnodulation in groundnut were carried out in a cross, NC 17×PI 259747, with a single Rhizobium strain, NC 92, and a native Rhizobium population.The normal nodulation of the parents, F₁ generations and backcross progenies, and the F₂ segregation for nodulation and nonnodulation confirmed that nonnodulation in groundnut is controlled by two duplicate recessive genes.Approved ICRISAT Journal Article No. 211.     

Components of resistance to late leaf spot and rust among interspecific derivatives and their significance in a foliar disease resistance breeding in groundnut (Arachis hypogaea L.)

Late leaf spot (LLS) and rust cause substantial yield losses and reduce the fodder and seed quality in groundnut (Arachis hypogaea L.). Adoption of resistant cultivars by the semi-arid tropic farmers is the best option to overcome yield losses. Knowledge on components of resistance to these diseases should facilitate the development of groundnut cultivars with enhanced resistance to LLS and rust. The objectives of the experiments were to study the genetic variability and relationships among components of resistance to LLS and rust, and assess their significance in disease resistance breeding. Fifteen interspecific derivatives for LLS and 14 for rust and a susceptible control, TMV 2, were evaluated in a randomised complete block design with two or three replications under greenhouse conditions. The experiments were repeated twice. Genotypic differences were highly significant for all the traits studied. Resistance to LLS is due to longer incubation and latent periods, lesser lesions per leaf, smaller lesion diameter, lower sporulation index, and lesser leaf area damage and disease score. Selection based on components of resistance to LLS may not lead to plants with higher retained green leaf area. The remaining green leaf area on the plant should, therefore, be the major selection criteria for resistance to LLS in breeding programs. Resistance to rust is due to longer incubation and latent periods, fewer pustules per leaf, smaller pustule diameter, lower sporulation index, and lesser leaf area damage and disease score. Rust resistant components appear to work additively, therefore, selection based on resistance components together with green leaf area retained on the plant should be the basis of selecting for resistance to rust in breeding programs. ICGV 99005, 99003, 99012, and 99015 for rust and ICGV 99006, 99013, 99004, 99003, and 99001 for LLS are the better parents for use in resistance breeding programs. This revised version was published online in July 2006 with corrections to the Cover Date.     

Assessment of genetic diversity among selected groundnut germplasm. I: RAPD analysis

Assessment of genetic diversity in a crop species is prerequisite to its improvement. The use of germplasm with distinct DNA profiles will help to generate genetically diversified breeding populations. The aims of the present experiment were to study molecular diversity among selected groundnut accessions and identify those with distinct DNA profiles for mapping and genetic enhancement. Twenty‐six accessions and eight primers of a 10‐mer were selected for random amplified polymorphic DNA assay. The genetic similarity (S_ij) ranged from 59.0% to 98.8%, with an average of 86.2%. Both multidimensional scaling and unweighted pair‐group method with arithmetic averages (UPGMA) dendrograms revealed the existence of five distinct clusters. However, this classification could not be related to known biological information about the accessions falling into different clusters. Some accessions with diverse DNA profiles (ICG 1448, 7101, and 1471, and ICGV 99006 and 99014) were identified for mapping and genetic enhancement in groundnut.     

Soil boron status: impact of lime and fertilizers in an Indian long-term field experiment on a Typic Paleustalf

In Indian agriculture, nitrogen (N) and phosphorus (P) fertilizers are predominantly used by the farmers, often ignoring secondary and micronutrients. Significance of boron (B) in nutrient management studies has been increasingly underlined under intensive cropping systems particularly in acid soils. In order to understand the distribution of soil native B in different fractions and their contribution to plant B uptake as influenced by nutrient management, soil samples collected after wheat (2009–2010) from a long-term experiment (LTE) continuing since 1972–1973 on Typic Paleustalf of Ranchi were subjected to sequential fractionation of soil B. Treatments included N alone, NP, NPK, 150% of recommended NPK, NPK + farmyard manure (FYM), NPK + lime, and an unfertilized-control. Five soil B fractions were determined along with hot CaCl₂-extractable (available) B. Averaged across the treatments, the soil had low organic carbon (C), pH and cation exchange capacity (CEC), and high free sesquioxides. Total B content was 21.7 mg kg^?1. Among different B fractions, residual B was the major contributor to total B and other fractions collectively shared 7% of total B only. Application of N alone depleted readily soluble, specifically adsorbed and organically bound B bringing the contents even below unfertilized-control. Conjoint use of lime or FYM with NPK increased significantly these fractions, whereas a decrease in oxide bound B was noticed under these treatments. Available B was positively correlated with these fractions indicating their significance in controlling B availability in the soil. The study revealed that use of lime or FYM helped modifying the distribution of soil B in different fractions by way of changing soil pH and organic C content, resulting in enrichment of plant available pool. A drastically low available B content in different treatments receiving fertilizers alone, however, suggested the necessity of B fertilization at prescribed rates for maintaining soil B fertility as also high crop yields.     

Long-term irrigation with zinc smelter effluent affects important soil properties and heavy metal content in food crops and soil in Rajasthan,India

Use of wastewater for irrigating agricultural crops is on the rise, particularly in the developing countries. The present study was undertaken to assess the long-term effect of irrigation with zinc smelter effluent on important soil properties including heavy metal status. Metal concentration in the edible parts of the crops grown on smelter effluent-irrigated soils was also measured. For this purpose, the agricultural lands which have been receiving the zinc smelter effluent irrigation for about five decades at Debari, Udaipur, India were selected. The adjacent tubewell water-irrigated fields were selected as reference. Long-term irrigation with smelter effluent resulted into significant buildup of ethylenediaminetetraacetic acid extractable Zn (57.7 fold), Cu (4.51 fold), Fe (3.35 fold), Mn (1.77 fold), Ni (1.20 fold), Pb (45.1 fold), and Cd (79.2 fold) in soils over tubewell water-irrigated fields. Total Zn, Cu, Mn, Ni, Pb, and Cd content in effluent-irrigated soils was also increased by 27.0, 1.60, 1.40, 1.30, 26.2, and 167 fold, respectively. Risk assessment indicated a very high to moderate potential ecological risk due to Cd, Pb, and Zn in soils close to the immediate vicinity of the smelter plant. Cadmium and Pb concentrations in edible parts of almost all the crops grown on effluent-irrigated soils were above the safe limit of CODEX commission. On an average, soil pH dropped by 0.31 units due to smelter effluent irrigation. Smelter effluent irrigation resulted into significant increase in soil organic carbon, electrical conductivity, and CaCO₃ content. On an average, there was decrease in available N (21.0%) and P (20.8%) content in effluent-irrigated soils over the tubewell water-irrigated ones. An increase in available K (102%) and S (26.0 fold) was recorded in effluent-irrigated soils. Long-term irrigation with zinc smelter effluent resulted into reduced microbial activities in soil as evidenced from the level of microbial biomass carbon and dehydrogenase activity. In view of the buildup of heavy metals and subsequent imbalance in essential plant nutrients in smelter effluent-irrigated soils, appropriate remediation-cum-fertilization strategy needs to be adopted for better soil health and plant nutrition.     

Developing Sustainability Indicators for Woody Biomass Harvesting in the United States

Bioenergy production has increased significantly in the last decade, and recent legislative efforts such as the discussion draft for the American Clean Energy and Security Act of 2009 and the Energy Independence and Security Act (EISA) of 2007 Energy Independence and Security Act. (2007). H. R. 6: 110th United States Congress. http://frwebgate.access.gpo.gov/cgi-bin/getdoc.cgi?dbname=110_cong_bills&docid=f:h6enr.txt.pdf (http://frwebgate.access.gpo.gov/cgi-bin/getdoc.cgi?dbname=110_cong_bills&docid=f:h6enr.txt.pdf)  [Google Scholar] are expected to encourage even more growth. The growing demand for bioenergy will necessitate production of large quantities of woody biomass and plant residues if it is to be met. However, concerns are being raised as to how increased pressures will affect the sustainability of woody biomass. In order to avoid potential pitfalls and ensure the sustainability of wood-based bioenergy systems, a set of sustainability indicators needs to be developed. Some of these indicators can be based on standards similar to those developed for sustainable forest management, energy balances, greenhouse gas emission reductions, and existing codes and guidelines for biomass harvesting. This article discusses a potential set of sustainability indicators encompassing ecological, economic, and social principles for harvesting woody biomass for bioenergy. The extent to which existing standards and certification systems reflect these indicators is elaborated upon. Methods for making these standards operational are also suggested.     

Economic and Life-Cycle Analysis of Forest Carbon Sequestration and Wood-Based Bioenergy Offsets in the Central Hardwood Forest Region of United States

This study investigates the combined impact of carbon and bioenergy markets on upland oak dominated mixed hardwood forests in the Central Hardwood Forest Region (CHFR) of the United States. A modification of the Hartman model was used for the economic analysis of carbon sequestration and using wood-based biomass for bioenergy. A life-cycle assessment was used to determine the amount of carbon sequestered due to stand growth and emitted during harvesting and decay of wood products. Two scenarios were taken, one where additionality of carbon is considered and the other where it is not. Sensitivity analysis was done with the range of carbon and bioenergy prices. The results show that net carbon payments have more impact on land expectation value (LEV) when additionality is not considered; in contrast, bioenergy payments have more impact on LEV when additionality is considered. Carbon and bioenergy prices also influenced the amount of stand level supply of forest products and carbon in both scenarios. In general, sawtimber, wood bioenergy, and carbon supply increased with an increase in carbon prices, whereas, pulpwood supply decreased. With few exceptions at higher carbon prices, bioenergy supply decreased with the increase in wood bioenergy prices, showing a backward bending supply curve in both scenarios.