RESEARCH PAPER: Segmental dorsolumbar epidural analgesia via the caudal approach using multiple port catheters with ketamine or lidocaine or in combination in cattle

ObjectiveTo determine the analgesic and systemic effects of epidural administration of ketamine, lidocaine or a combination of ketamine/lidocaine in standing cattle.Study designProspective, randomized, experimental trial.AnimalsSix healthy male cattle weighing between 335 and 373 kg.MethodsThe animals received 0.5 mg kg^?1 of ketamine (K), 0.2 mg kg^?1 of 2% lidocaine (L) or 0.25 mg kg^?1 ketamine plus 0.1 mg kg^?1 lidocaine (KL). All the drugs were injected into the dorsolumbar epidural space via a caudal approach through a non‐styletted multiple‐port catheter. Each animal received each treatment at random. Evaluations of analgesia, sedation, ataxia, heart rate, arterial pressure, respiratory rate, skin temperature and rectal temperature were obtained at 0 (basal), 5, 10, 15, 30, 45, 60, 75, 90 minutes after epidural injection, and then at 30‐minute intervals until loss of analgesia occurred. Skin temperature was taken at these intervals up to 60 minutes. All the animals received a standard noxious stimulus; a 4‐point scale was used to score the response. A second scale was used to score ataxia and a third for sedation.ResultsThe duration of analgesia in the upper and lower flanks in cattle was 140 ± 15, 50 ± 14 and 80 ± 22 minutes (mean ± SD) after dorsolumbar epidural KL, K or L, respectively. The cardiovascular changes were within acceptable limits in these clinically healthy cattle.ConclusionsDorsolumbar epidural administration of KL to cattle resulted in longer duration of analgesia of the upper and lower flanks in standing conscious cattle, than the administration of K or L alone.Clinical relevanceFurther research is necessary to determine whether this combination using this technique provides sufficient analgesia for flank surgery in standing cattle.     

Guava decline: effect of root exudates from Meloidogyne enterolobii-parasitized plants on Fusarium solani in vitro and on growth and development of guava seedlings under controlled conditions

In guava decline, Fusarium solani-immune guava trees become susceptible to extensive root rot caused by this fungus after parasitism by Meloidogyne enterolobii. To understand the mechanisms involved in this disease, root exudates were collected from nematode-inoculated (NI) or uninoculated (UN) guava plants cultivated in sand. After filtration through a Millipore® membrane, NI and UN exudates were used: i) to prepare media to assess their effect on mycelial growth and production of propagules of F. solani isolate UENF/CF 163, and ii) to incubate macro- and microconidia to assess their effect on germination. NI exudates promoted (P?<?0.05) more mycelial growth and production of propagules than UN exudates or water. NI and UN exudates were used to water guava seedlings laid over seed germination paper inside plastic boxes. Half of the seedlings had an agar plug colonized by the fungus positioned in the collar region. Upon watering with NI exudates the fungus caused (P?<?0.05) extensive rotting of the seedlings’ rootlets. NI and UN exudates, either unlyophilized or lyophilized and re-suspended to the original concentration, were used to water guava seedlings grown in sterile sand before being inoculated (or left uninoculated) as described before. Solely upon watering with NI exudates, in its unlyophilized form or after lyophilization, the fungus caused a reduction (P?<?0.05) of shoot and root biomass associated with rotting of roots. These results suggest that M. enterolobii induces chemical changes in the root exudates of guava trees, which are necessary for root invasion causing root rotting by F. solani.     

Salix rubens and Salix triandra Species as Phytoremediators of Soil Contaminated with Petroleum-Derived Hydrocarbons

The petroleum industry activities provide potential risks to the environment because they can contaminate ecosystems with different organic compounds in the production chain. Several accidents with transport and handling of petroleum and related products occurred in urban areas with harmful effects to the quality of life and economy. In the 1990s, bioremediation and phytoremediation technologies as economically feasible alternatives to repair the environmental damage were developed. In this study, the potential of the willows Salix rubens and Salix triandra were evaluated with regard to the phytoremediation of soils contaminated with petroleum-derived hydrocarbons (total hydrocarbons and polycyclic aromatic hydrocarbons (PAHs)). The PAHs were quantified by extraction from soils and plants using dichloromethane under ultrasonication. The HPLC analysis was performed with GC/MSD equipment. The total hydrocarbons present in uncontaminated soil were quantified by the sum of animal/vegetable oils and greases and mineral oils and greases according to Standard Methods 5520 (1997). The two willows species S. rubens and S. triandra were resistant during the project development. In the contaminated soil, in which both species were planted, the total hydrocarbons concentration was reduced near 98?%. The PAHs content was remarkably reduced as well. Pyrene showed an initial concentration of 23.06???g?kg^?1, decreasing in most cases to 0.1???g?kg^?1 or to undetectable levels. Chrysene decreased from 126.27???g?kg^?1 to undetectable levels. Benzo[k]fluoranthene and benzo[a]pyrene concentrations had also showed a decrease from 28.44 and 3.82???g?kg^?1, respectively, to undetectable levels.     

Genetic parameters for body weight, reproduction, and parasite resistance traits in the Creole goat

We estimated the genetic parameters for BW, reproduction, and parasite resistance traits to implement a breeding program for the Creole goat. The traits were preweaning BW at 70 d of age (BW70d), BW at 11 mo of age (BW11), fecal egg count at 11 mo of age (FEC11) for all animals, packed cell volumes of lactating does (PCV), and their fertility (FER) and litter size (LS). We analyzed about 30 yr of data, which included 18,450 records on 11,970 animals from the INRA experimental flock in Guadeloupe (French West Indies). Heritability estimates were low for reproduction traits (0.11 ± 0.02 for LS and FER) to moderate for production traits (0.32 ± 0.03 for BW11; 0.20 ± 0.03 and 0.08 ± 0.02 for the direct and maternal heritability estimates of BW70d, respectively). Heritability estimates for gastrointestinal nematode resistance traits were situated in an intermediate range (0.13 ± 0.05 for PCV and 0.18 ± 0.04 for FEC11). Genetic correlations between FER, PCV, BW11, and the maternal effect of BW70d were altogether positive, whereas LS and FEC11 were almost uncorrelated phenotypically and genetically. These correlations are very favorable for setting up a breeding program, making it possible to improve BW, reproduction, and parasite resistance traits simultaneously.     

A modified agglutination test for the diagnosis of Besnoitia besnoiti infection

Bovine besnoitiosis is caused by Besnoitia besnoiti, an obligate intracellular apicomplexan parasite. Affected animals present cutaneous and systemic manifestations and the disease may lead to considerable economic losses. Although generally associated to tropical and subtropical areas, bovine besnoitiosis is now considered an emergent disease in Europe, due to the increasing number of new cases and apparent geographical expansion. In this study we evaluated the performance of a modified agglutination test (B-MAT) in the serodiagnosis of bovine besnoitiosis in comparison to the indirect immunofluorescent-antibody test (IFAT). To establish optimal protocol conditions we used bovine sera with a known infection status for B. besnoiti infection. Positive animals (n=36) presented B. besnoiti dermal cysts and anti-B. besnoiti specific antibodies, as determined by the indirect immunofluorescence test (IFAT). Negative animals (n=103) were from non-endemic areas in Portugal and negative by the IFAT. From here, we evaluated the sensitivity and specificity of the B-MAT relative to the IFAT with a panel of sera from herds with history of bovine besnoitiosis in Portugal, Spain and France (n=402), using three serum dilutions (1:80, 1:160, 1:320). Considering the positive cut-off at 1:160 serum dilution, the B-MAT showed an almost perfect test agreement with the IFAT; (κ=0.968; 95% CI: 0.941-0.996) with a relative sensitivity of 97.2% (95% CI: 94.1-100%) and a relative specificity of 99.3% (95% CI: 98.4-100%). As a simple and inexpensive technique the B-MAT represents a valuable tool for the diagnosis and study of the epidemiology of bovine besnoitiosis.     

Bioremediation of Mangroves Impacted by Petroleum

The majority of oil from oceanic oil spills (e.g. the recent accident in the Gulf of Mexico) converges on coastal ecosystems such as mangroves. Microorganisms are directly involved in biogeochemical cycles as key drivers of the degradation of many carbon sources, including petroleum hydrocarbons. When properly understood and managed, microorganisms provide a wide range of ecosystem services, such as bioremediation, and are a promising alternative for the recovery of impacted environments. Previous studies have been conducted with emphasis on developing and selecting strategies for bioremediation of mangroves, mostly in vitro, with few field applications described in the literature. Many factors can affect the success of bioremediation of oil in mangroves, including the presence and activity of the oil-degrading microorganisms in the sediment, availability and concentration of oil and nutrients, salinity, temperature and oil toxicity. More studies are needed to provide efficient bioremediation strategies to be applicable in large areas of mangroves impacted with oil. A major challenge to mangrove bioremediation is defining pollution levels and measuring recuperation of a mangrove. Typically, chemical parameters of pollution levels, such as polycyclic aromatic hydrocarbons (PAHs), are used but are extremely variable in field measurements. Therefore, meaningful mangrove monitoring strategies must be developed. This review will present the state of the art of bioremediation in oil-contaminated mangroves, new data about the use of different mangrove microcosms with and without tide simulation, the main factors that influence the success of bioremediation in mangroves and new prospects for the use of molecular tools to monitor the bioremediation process. We believe that in some environments, such as mangroves, bioremediation may be the most appropriate approach for cleanup. Because of the peculiarities and heterogeneity of these environments, which hinder the use of other physical and chemical analyses, we suggest that measuring plant recuperation should be considered with reduction in polycyclic aromatic hydrocarbons (PAHs). This is a crucial discussion because these key marine environments are threatened with worldwide disappearance. We highlight the need for and suggest new ways to conserve, protect and restore these environments.     

Reduced Atmospheric Manganese in Montreal Following Removal of Methylcyclopentadienyl Manganese Tricarbonyl (MMT)

Methylcyclopentadienyl manganese tricarbonyl (MMT) was used between 1990 and 2003 as an antiknock agent and as an octane booster in Canadian unleaded gasoline. Its combustion leads to Mn emissions. The objective of this research was to examine the variations in atmospheric Mn in Montreal (Canada) from 2001 to 2007, covering the period prior (2001?C2003) to and following (2005?C2007) MMT use. Three sampling stations were selected because of their proximity to roads with widely differing and well-known traffic patterns. Filters from 2001 to 2007 were obtained from the Montreal Urban Community. The first sample of each month was selected, and Mn analysis was performed by neutron activation analysis. Total suspended particulates (TSP) was calculated by weighing the filters before and after dust collection. Results show a significant decrease of Mn over time at each station, whereas TSP decreased significantly in two stations. Comparing atmospheric Mn during and after the period of use of MMT 2001?C2003 vs 2005?C2007 showed a significant decrease at all stations. For TSP, only one station showed borderline significant decline between these two periods. Overall, between the two periods, Mn and TSP decreased by 39% and 17%, respectively. These data suggest that the combustion of MMT led to an increase of airborne Mn of approximately 22%. These findings should help in decision-making processes concerning the use of MMT in gasoline in other countries.     

Comparison of different intracellular cryoprotectants on the solid surface vitrification of red-rumped agouti (Dasyprocta Leporina Lichtenstein, 1823) ovarian tissue

In contributing to the conservation of wild rodents, the aim of this study was to evaluate the use of distinct cryoprotectants, separately or in combination, for solid surface vitrification (SSV) of red-rumped agouti ovarian tissue. Ovarian cortex from nine females was recovered and fragmented. Fresh fragments (control) were used to analyse the pre-antral follicle (PF) morphology using a histologic procedure, viability using the Trypan blue test, cell proliferation by counting the argyrophilic nucleolar organizing regions (Ag-NORs technique) and DNA integrity using the TUNEL assay. The remaining fragments were vitrified using SSV method with 3 M or 6 M ethylene glycol (EG) or dimethyl sulfoxide (DMSO), or in combination (3 M EG/3 M DMSO), and further evaluated as reported for the fresh samples. All cryoprotectants were effective at preserving PFs morphology compared to the control group (80.7 ± 5.21%), except 6 M EG and 3 M DMSO that provoked a significant (p < .05) decrease on the values of morphologically normal primary (60.0 ± 19.0%) and primordial (44 ± 4.5%) follicles, respectively. Regarding viability, all cryoprotectants provided values similar to that verified for the control group (79.0%), but a significant decrease (p < .05) was observed with EG/DMSO combination (59%). Using Ag-NORs technique, the highest (p < .05) cell proliferative capacity was detected when using EG at each tested concentration. The TUNEL proved the preservation of DNA integrity regardless of the cryoprotectant. In summary, we suggest the use of 3 M EG for the solid surface vitrification of red-rumped agouti ovarian tissue.     

A multiple-phenotype imputation procedure as a method for prediction of cheese-making efficiency in Spanish Assaf sheep

Sheep milk is mainly intended to manufacture a wide variety of high-quality cheeses. The ovine cheese industry would benefit from an improvement, through genetic selection, of traits related to the milk coagulation properties (MCPs) and cheese yield-related traits, broadly denoted as “cheese-making traits.” Considering that routine measurements of these traits needed for genetic selection are expensive and time-consuming, this study aimed to evaluate the accuracy of a cheese-making phenotype imputation method based on the information from official milk control records combined with the pH of the milk. For this study, we analyzed records of milk production traits, milk composition traits, and measurements of cheese-making traits available from a total of 1,145 dairy ewes of the Spanish Assaf sheep breed. Cheese-making traits included five related to the MCPs and two cheese yield-related traits. The milk and cheese-making phenotypes were adjusted for significant effects based on a general linear model. The adjusted phenotypes were used to define a multiple-phenotype imputation procedure for the cheese-making traits based on multivariate normality and Markov chain Monte Carlo sampling. Five of the seven cheese-making traits considered in this study achieved a prediction accuracy of 0.60 computed as the correlation between the adjusted phenotypes and the imputed phenotypes. Particularly the logarithm of curd-firming time since rennet addition (logK₂₀) (0.68), which has been previously suggested as a potential candidate trait to improve the cheese ability in this breed, and the logarithm of the ratio between the rennet clotting time and the curd firmness at 60 min (logRCT/A60) (0.65), which has been defined by other studies as an indicator trait of milk coagulation efficiency. This study represents a first step toward the possible use of the phenotype imputation of cheese-making traits to develop a practical methodology for the dairy sheep industry to impute cheese-making traits only based on the analysis of a milk sample without the need of pedigree information. This information could be also used in future planning of specific breeding programs considering the importance of the cheese-making efficiency in dairy sheep and highlights the potential of phenotype imputation to leverage sample size on expensive, hard-to-measure phenotypes.