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排序方式: 共有413条查询结果,搜索用时 15 毫秒
111.
Dario D'Ovidio Giacomo Rossi Raffaele Melidone Francesca Menna Alessandro Fioretti 《Journal of Exotic Pet Medicine》2012,21(3):238-242
A 6-year-old spayed female ferret (Mustela putorius furo) exhibiting clinical signs of weakness, anorexia, lethargy, weight loss, and frequent urination was presented for a veterinary evaluation to determine the underlying cause of the aforementioned abnormal behavior and clinical condition. Physical examination revealed a large, firm, painless, movable subcutaneous mass at the base of the tail. Radiographic and ultrasound images confirmed the presence of a soft tissue mass compressing the sacrococcygeal vertebrae, but there was no evidence of metastatic lesions. Because of the poor prognosis and progressive deterioration of the animal's clinical condition, the ferret was humanely euthanized. Gross necropsy revealed a 4 × 4 × 3.5 cm, firm, yellow-tan, ovoid, subcutaneous mass wrapped around the rectum and the anus. The mass did not appear to breach the serosa. Evaluation of the abdominal cavity revealed a pale yellow liver, possibly associated with hepatic lipidosis with no gross evidence of metastasis in the body cavity. The histopathological features of the mass were consistent with a liposarcoma. To the best of the authors' knowledge, this is the first case of subcutaneous liposarcoma reported in a ferret. 相似文献
112.
Peli Angelo Spadari Alessandro Romagnoli Noemi Bettini Giuliano Scarpa Filippo Pietra Marco 《Journal of veterinary science (Suw?n-si, Korea)》2009,10(3):265-267
A not pregnant 4-year-old Jersey cow was presented with the sudden appearance of respiratory noise, nasal discharge and moderate respiratory difficulty. Upon physical examination a snoring-like noise, extended head and neck position, exaggerated abdominal effort, bilateral nasal discharge and left prescapular lymph node enlargement were noted. Sub-occlusion of the initial portion of the respiratory tract was suspected. Radiographic and endoscopic examinations revealed a pedunculate mass on the dorsal aspect of the rhinopharynx, which was removed with endoscopically assisted electrosurgery. Histologic examination revealed a chronic pyogranulomatous inflammation with eosinophilic club-like bodies surrounding small colonies of rod-shaped bacteria. Results of histochemical staining were consistent with Actinobacillus-like bacteria and a diagnosis of respiratory actinobacillosis was reached. Surgery and antibiotic therapy were resolutive, as demonstated by an endoscopic check at the second month after surgery, even without the association of the traditional iodine cure, which is regarded as the treatment of choice for actinobacillosis. 相似文献
113.
114.
Roberto Cardelli Michelangelo Becagli Fausto Marchini Alessandro Saviozzi 《Soil Use and Management》2019,35(3):478-481
This study was carried out in order to assess the influence of biochar applications on the estimation of colorimetric‐based enzymatic assays and to verify the effectiveness of the most common methods. Since most methods used to determine enzymatic activities in the soil are based on colorimetry, biochar may absorb substrates and/or coloured products thereby distorting the analytical result. Biochar was added to two soils, with different textures and cation exchangeable capacities, at a rate of 2% (w/w), and seven enzyme activities were determined following standard methods. The biochar amendment lowered the spectrophotometer reading of the activity of FDAase and dehydrogenase in the sandy soil. In the three enzymatic activities based on p‐nitrophenol production (β‐glucosidase, phosphatase and arylsulphatase), the addition of biochar did not change the enzyme assays. The biochar led to an overestimation in terms of the protease and urease activities in the sandy soil. In the clay loamy soil, biochar did not change the response of any of the enzyme activities tested. A biochar dose of up to 2% only guarantees the effectiveness of the most common spectrophotometric methods for not excessively sandy soils. 相似文献
115.
116.
Richard R. Stange Jr Rocco Alessandro T. Greg Mc Collum Richard T. Mayer 《Physiological and Molecular Plant Pathology》2002,60(6):283
Pathological tissues from a variety of plants turn red when treated with the histochemical reagent phloroglucinol (PG)–HCl. This induced PG–HCl reacting material has been termed both wound gum and, more recently, induced lignin or lignin-like material. We are exploring alternative approaches to better identify this induced material. In this report, we describe methods for purifying the PG–HCl reactive material from solvent-washed tissue preparations and measuring the amount of p -coumaryl aldehyde released from the purified material by boiling in water. Acorn squash fruit wall tissue was sliced, sprayed with pectinase to elicit the formation of PG–HCl reactive material and incubated up to 72 h at 27°C. Sampled tissue was washed extensively with water and organic solvents. Insoluble residues were treated with cell wall degrading enzymes to remove cell wall materials, and dimethyl sulfoxide was used to dissolve starch. Yields of residual material increased from 7.5% in the time zero samples to 29.7% after 72 h. Refluxing the purified material in water released <6 μ g p -coumaryl aldehyde g−1 from preparations of tissue at time zero and 8100 μ g p -coumaryl aldehyde g−1 from samples incubated for 48 h. These results suggest that the function of the material is to sequester phytoalexins, but are not consistent with the material being lignin. Published by Elsevier Science Ltd. 相似文献
117.
Gargiulo A Sensale M Marzocco L Fioretti A Menna LF Dipineto L 《Veterinary microbiology》2011,150(3-4):401-404
To evaluate the presence of Campylobacter spp. and related cdt genes, cloacal swabs were collected from 70 common teals (Anas crecca) and analyzed by culture methods and polymerase chain reaction. In addition, C. jejuni were examined also for the presence of wlaN gene. This is believed to be the first report of Campylobacter spp. in common teal and our results confirm the very common occurrence of C. jejuni (n=40) and C. coli (n=13) in waterfowls. Furthermore, the cdt genes were frequently present in both C. jejuni and C. coli isolated. Moreover, seven C. jejuni isolates carried also the wlaN gene which is presumably involved in the expression of ganglioside mimics in Guillain-Barré syndrome. 相似文献
118.
Chiocchetti R Grandis A Bombardi C Clavenzani P Spadari A Gentile A Bortolami R 《American journal of veterinary research》2005,66(4):710-720
OBJECTIVE: To determine the location, morphology, and neurochemical code of spinal cord and dorsal root ganglion neurons that innervate the gastrocnemius muscle (GM) and superficial digital flexor muscle (FDSM) in cattle. ANIMALS: 5 healthy Friesian male calves. Procedure: 2 different types of neuronalretrograde fluorescent tracers (fast blue and diamidino yellow) were injected into the GM and FDSM, respectively. The neurochemical code (substance P, calcitonin gene-related peptide, galanin, and neuronal nuclear protein) of labeled neurons was investigated by immunohistochemistry. RESULTS: Neurons innervating the GM and FDSM were located along the L6-S2 spinal cord segments and ganglionic levels. A cranial-to-caudal topographic distribution for each muscle was found, indicating that the motor nuclei of the 2 muscles are organized by a somatotopic pattern. The GM and FDSM motoneurons were immunoreactive only for calcitonin gene-related peptide, whereas the afferent neurons were immunoreactive for all of the neurochemical markers considered. CONCLUSION AND CLINICAL RELEVANCE: In our study, location and the extent of neurons that supply the GM and FDSM of cattle were characterized completely. Because the GM and FDSM are involved in spastic paresis of calves and it is thought that spastic paresis results from an excessive activity of the neuromuscular spindle reflex arc, findings in our study may be useful for further electrophysiologic and clinical studies. Knowledge of the neurochemical code of neurons that supply the GM and FDSM in healthy calves could be used to compare chemical alterations in the same neuronal population of affected calves. 相似文献
119.
A case of a feline Merkel cell tumour is described. An 8-year-old, female cat developed a round, alopecic, reddish mass on the nose. Wide excisional surgery was performed with cartilage resection. Histologically the mass was composed of solid islands of mostly basophilic densely packed cells with a scant cytoplasm, which was suggestive of a neuroendocrine origin. Results of immunohistochemical studies using antibodies against neurone-specific enolase, chromogranin, synaptophysin and pan-cytokeratin allowed classification of the lesion as a Merkel cell tumour. Ultrastructurally, dense core granules were identified in the cytoplasm. In a 2-year follow-up no relapses or metastases were observed. The clinical course recorded is in contrast with the malignant nature of a Merkel cell tumour recently described in a cat and of the human Merkel cell tumour, but is similar to the course of the canine Merkel cell tumour which is often benign. Early diagnosis along with the use of wide surgical excision might be considered an important factor in preventing relapse of this tumour. 相似文献
120.
Genchi C Rossi L Cardini G Kramer LH Venco L Casiraghi M Genchi M Agostini A 《Veterinary parasitology》2002,110(1-2):85-91
The authors report the efficacy of an injectable, moxidectin sustained release (SR) formulation for the prevention of canine heartworm infection in endemic areas in northern and central Italy. Three field trials were carried out on a total of 324 dogs. Two hundred forty-three dogs were treated with moxidectin SR 6 months apart and 81 dogs (positive controls) with moxidectin tablets given monthly for 5 consecutive months during the risk season each year throughout the study. Results of testing for microfilariae and circulating adult female antigens were negative for all the experimentally treated dogs at the 6, 7, 11 and 19 months after the last injection. No adverse reactions to moxidectin SR were observed but a moderate pain at palpation and swelling (5-6 cm) at the injection site after the first treatment. In the study areas, prevalence of Dirofilaria immitis infection calculated by testing dogs which had no preventive treatment in the previous transmission season ranged from 33 to 63%. This study confirms the efficacy and safety of injectable, moxidectin SR formulation in the prevention of heartworm infection in dogs and demonstrates that the prophylactic efficacy lasts for the full season and strongly suggests that the product gives 1-year protection. 相似文献