Effects of dietary arginine on hematological parameters and innate immune function of channel catfish

The effects of elevated dietary arginine on the hematology and immune function of juvenile channel catfish Ictalurus punctatus were evaluated by means of in vivo and in vitro experiments. Healthy juvenile channel catfish (average weight, 34.8 g) were fed casein-gelatin-based diets containing 28% crude protein and supplemented with crystalline L-arginine (ARG) at 0.5, 1, 2, or 4% of diet. An intact-protein diet containing 1.3% arginine also was included to investigate the effects of amino acid form (crystalline-free amino acids versus intact protein). Each purified diet was fed to apparent satiation to triplicate groups of fish for 6 weeks. At the end of the experimental feeding period, the fish were injected intraperitoneally with two doses (3 d apart) of 2 mg lipopolysaccharide/kg body weight. Six days after the initial injection, the fish were anesthetized and tissue samples were obtained to evaluate hematological and humoral and cellular immune parameters, including phagocytic activity of peritoneal macrophages, hemoglobin, hematocrit, mean corpuscular volume (MCV), blood cell counts, plasma protein, and hepatic superoxide dismutase activity. High dietary levels (4% ARG) resulted in significantly higher levels of hemoglobin, hematocrit, and circulating erythrocytes. Dietary ARG did not significantly affect MCV and the number of circulating leukocytes, lymphocytes, eosinophils, and monocytes. In vitro, a moderate level (2 mM) of ARG in the culture media was found to be ideal in significantly enhancing phagocytosis. This study demonstrates that some aspects of the immune system of channel catfish are sensitive to changes in dietary ARG.     

Effect of Dietary Supplementation of Acidic Calcium Sulfate (Vitoxal) on Growth,Survival, Immune Response and Gut Microbiota of the Pacific White Shrimp,Litopenaeus vannamei

Dietary acidifiers have been recognized as beneficial in animal production including aquacultural production of fish where they confer such benefits as improved feed utilization, growth, and resistance to bacterial pathogens. If improvements in growth and immune responses by acidifier supplementation can be confirmed in shrimp, then mortalities due to diseases could be minimized, limiting the emergence of disease‐resistant bacterial pathogens as a potential result of antibiotic misuse. With this in mind, a 35‐d feeding trial was conducted to evaluate growth, enteric microbiota populations, and nonspecific immune responses of Litopenaeus vannamei fed diets containing the commercial acidifier Vitoxal, based on acidic calcium sulfate in an indoor temperature‐controlled, recirculating culture system without any natural productivity. Experimental diets were formulated to contain 0 (basal), 0.4, 1.2, 1.6, or 2.0% acidic calcium sulfate (ACS) by weight. Shrimp fed in excess, 15 times a day using automatic feeders. Weight gain and survival among treatments were excellent, but not significantly different (P > 0.05). Denaturing gradient gel electrophoresis analysis revealed that the enteric microbial community of shrimp fed the basal diet differed markedly from those fed the acidifier on the basis of 64.9% similarity coefficient. Shrimp fed the commercial acidifier at 1.2 and 2.0% responded significantly (P < 0.05) better to reduced stress and displayed enhanced immune responses including hemocyte phagocytic capacity, hemolymph protein concentration, hyaline cell counts, and hemolymph glucose, compared with shrimp fed the basal diet. These results point to an enhanced performance in terms of positive shifts in the composition of enteric microbial communities as well as improved immune performance, with no changes in growth or survival.     

Macroalgal meadow habitats support fish and fisheries in diverse tropical seascapes

Canopy‐forming macroalgae can construct extensive meadow habitats in tropical seascapes occupied by fishes that span a diversity of taxa, life‐history stages and ecological roles. Our synthesis assessed whether these tropical macroalgal habitats have unique fish assemblages, provide fish nurseries and support local fisheries. We also applied a meta‐analysis of independent surveys across 23 tropical reef locations in 11 countries to examine how macroalgal canopy condition is related to the abundance of macroalgal‐associated fishes. Over 627 fish species were documented in tropical macroalgal meadows, with 218 of these taxa exhibiting higher local abundance within this habitat (cf. nearby coral reef) during at least one life‐history stage. Major overlap (40%–43%) in local fish species richness among macroalgal and seagrass or coral reef habitats suggest macroalgal meadows may provide an important habitat refuge. Moreover, the prominence of juvenile fishes suggests macroalgal meadows facilitate the triphasic life cycle of many fishes occupying diverse tropical seascapes. Correlations between macroalgal canopy structure and juvenile abundance suggests macroalgal habitat condition can influence levels of replenishment in tropical fish populations, including the majority of macroalgal‐associated fishes that are targeted by commercial, subsistence or recreational fisheries. While many macroalgal‐associated fishery species are of minor commercial value, their local importance for food and livelihood security can be substantial (e.g. up to 60% of landings in Kenyan reef fisheries). Given that macroalgal canopy condition can vary substantially with sea temperature, there is a high likelihood that climate change will impact macroalgal‐associated fish and fisheries.     

Masculinization of the Native Bay Snook,Petenia splendida,Using Oral Administration of the Synthetic Steroid 17α‐methyltestosterone

The possibility of inducing sex reversal in bay snook, Petenia splendida, was evaluated to generate a protocol aimed at producing single‐sex populations (100% males) and improving farming conditions for this species. This popular staple food is a cichlid fish native to Central America, with aquaculture potential. We evaluated oral administration of the synthetic steroid 17α‐methyltestosterone (MT) to determine optimal dosage (30, 45, and 60 mg/kg of food) and days of feeding (30, 45, and 60) to attain efficient masculinization. The statistical analysis indicates a highly significant effect of both factors as well as for the interaction (P < 0.01). We registered significant differences in growth at the end of the experiment; fish fed with 60 mg of MT for 60 d reached the largest size, while fish fed with no MT were smaller. No significant differences were observed for survival (P > 0.05), ranging from 55.7 to 75.3% in the treatments. We recommend the use of 60 mg/kg of MT for 30 d for achieving successful sex reversal. This study provides valuable information supporting the inclusion of new species in aquaculture, reducing time and costs associated with sex reversal previously proposed for the species.     

Unusual Microstructure and Mechanical Properties of Egg Case of the Bolas Spider <Emphasis Type="Italic">Mastophora corpulenta</Emphasis> Banks (Araneae,Araneidae)

Bolas spiders of the genus Mastophora are very uncommon orb-weaver spiders, famous for their unusual predatory behavior. Being strictly nocturnal and very cryptic, they are extremely difficult to reveal, which explains their rarity in world arachnological collections. After a recent finding of an adult female of Mastophora corpulenta Banks (one of especially rare representatives of the genus), a few egg cases of this species became available, which seem to be very different from those of more common spiders due to their unusual hardness and coloration. We characterized some spectral, structural, and mechanical properties of this unusual silk material, by using Fourier-transform infrared spectroscopy, scanning electron microscopy (SEM), 3D profilometer imaging, and atomic force microscopy (AFM). For comparison, similar studies were performed for egg cases of the Southern black widow Latrodectus mactans Fabricius, since spiders of the genus Latrodectus are known to produce silk fibers of comparable toughness. Some similarities were observed between the silks produced by Mastophora corpulenta and Latrodectus mactans in terms of the size and chemical composition of egg cases. At the same time, SEM imaging and AFM force characterization revealed striking differences between them, due to the fact that Mastophora egg cases, unlike those produced by more common Araneae species, includes a solid (fused) wall, lacking evident porous structure, which results in their outstanding stiffness and strength.     

Interacting effects of soil degradation and precipitation on plant productivity in NE Patagonia,Argentina

Our objective was to examine the effects of inter-annual variation of precipitation on productivity of two dominant species (Chuquiraga avellanedae, an evergreen shrub, and Nassella tenuis, a perennial grass) in two communities of contrasting soil degradation: a herbaceous steppe with shrubs (HSS) and a degraded shrub steppe (SS). Data were collected during two consecutive years with different annual precipitation. Aboveground productivity was determined nondestructively using a double sampling approach. The number of inflorescences per plant was recorded too. Perennial grass productivity was lower in SS than in HSS in both years, while shrub productivity was lower in SS only during the year of below average precipitation. With rising precipitation the perennial grass increased the number of inflorescences while the evergreen shrub augmented vegetative biomass. In summary, the effects of precipitation on plant productivity are community dependent; abiotic factors, such as superficial and sub-superficial soil characteristics, and biotic factors, such as leaf area index (LAI) or tussock sizes, may interact to influence the responses of species to precipitation. Our results suggest that if precipitation increased, this would favor the dominance of shrubs over grasses.     

A survey of Lolium rigidum populations in citrus orchards: Factors explaining infestation levels

The presence of herbicide‐resistant Lolium rigidum in Mediterranean (Spanish) citrus orchards was reported in 2005 and it poses a serious threat to crop management. The main objective of this research was to investigate which components could be responsible for the persistence of annual ryegrass populations in Mediterranean mandarin and orange orchards. This is the first study regarding L. rigidum populations in Mediterranean citrus orchards. Surveys were conducted in 55 commercial citrus orchards in eastern Spain in 2013 by interviewing technicians who were working in cooperatives about crop management. The level of infestation by L. rigidum and the presence of harvester ants (Messor barbarus) then were estimated in the same orchards. The variables were subjected to a two‐dimensional analysis and both univariate and multivariate logistic regression models were fitted for each of the three L. rigidum density levels that had been established. The multivariate models showed the significant factors that were associated with various L. rigidum densities: (i) at a low density, the herbicides that were applied, the number of applications in 2013 and the type of irrigation (flood or drip); (ii) at a medium density, the presence of harvester ants; and (iii) at a high density, the herbicides that were applied in 2013. The results indicated that drip irrigation and one application of glyphosate mixed with other herbicides (or herbicides other than glyphosate) were associated with a lower L. rigidum density. The alternative management options that are presented here should help farmers to reduce weed problems in Mediterranean citrus orchards. Future research is required to better understand the presence of herbicide‐resistant populations, as well as the possible beneficial presence of granivorous ant species.