Dietary Aloe vera components’ effects on cholesterol lowering and estrogenic responses in juvenile goldfish, Carassius auratus

Aloes are now considered a very interesting source of bioactive compounds among which phytosterols should play a major role. The present study is an attempt to investigate the hypocholesterolemic activity of Aloe vera associated with its impact on the reproductive status of juvenile goldfish. Therefore, the short- and long-term effects of feeding supplementary diet containing aloe components (20 mg aloe/g diet; 2 %) on plasma lipids, plasma vitellogenin, and hepatic estrogen receptor α/β1 mRNA levels in goldfish were examined. Results of GC–MS for phytosterols show high abundance of β-sitosterol in freeze-dried powder of Aloe vera whole leaves. Moreover, a 2 % aloe powder dietary supplement was not found estrogenic in juvenile goldfish after either 7- or 30-day treatment, but was consistent in plasma hypocholesterolemic effects following long-term exposure. The present data further support that plasma cholesterol modulation induced by phytosterols may not be related to estrogen-like activity.     

Grazing effect on soil properties in conventional and no-till systems

Diversification of production is a concern for farmers in many regions of the world, raising a renewed interest in crop-animal rotations. However little information is available on whether the introduction of grazing animals in a no-till system could be a sustainable practice. The present long-term study was carried out in the semiarid region of Argentina, on an Entic Haplustoll (A, AC, C and C_k profile). The experimental plots were established in August 1993, with two treatments, no-till (NT) and conventional tillage (CT). Stubble was regularly used for grazing until 2002, when plots were divided into grazed (G) and non-grazed (NG) sub-treatments. Soil samples were taken at 0–0.10 and 0.10–0.20 m depth at the beginning of the experiment (1993) and during 2007, with the following determinations: clay + silt contents, bulk density (BD), total carbon (C), total nitrogen (N), available P, C contents of aggregate fractions of 2000–100 (POC), 100–50 (IOC) and <50 (FOC) μm diameter, aggregate size distribution and mean weight diameter change. NT showed a strong effect on all analyzed soil attributes: it had higher total carbon stocks (NT 16.6 Mg ha⁻¹ vs. CT 13.2 Mg ha⁻¹) and higher amounts in all C fractions, even in FOC (11.3 Mg ha⁻¹ vs. 9.2 Mg ha⁻¹). For BD, we found no difference between NT and CT at the surface and an even lower value for NT at 0.10–0.20 m depth. Under NT no depletion of available P occurred, while CT lost about 23 kg ha⁻¹. Grazing had a negative effect on BD when averaging BD data across tillage systems, while there was no effect on aggregate stability, and a positive one on the proportion of >8 mm aggregates (23.3% vs. 11.7% for CT G and CT NG, respectively). C stratification showed a differential effect of grazing: NT G had the highest index (1.31) and CT G the lowest one (0.98). Our results indicated that the introduction of grazing animals in NT crop systems would not be detrimental to soil conditions and quality, at least in semiarid conditions of Argentina.     

Effects of Yeast Freezing in Frozen Dough

The effects of freezing and frozen storage of bread dough and compressed yeast on bread quality were studied. Besides, the effects of compressed yeast freezing on cell viability, gas production and release of substances by the yeast cells were examined. Freezing and frozen storage of dough made with fresh yeast had more negative effects on baking quality than the addition of frozen yeast to dough. When the compressed yeast is frozen and stored at ‐18°C, the CO₂ production decreased, while the amount of dead cells, the total protein, and the total reducing substances leached from the yeast increased as the length of yeast frozen storage increased. SDS‐PAGE showed that the substances leached from frozen yeast caused an increase in the solubility of some gluten proteins. On the other hand, size‐exclusion chromatography (SEC) pointed out that the relative amount of two protein fractions of low molecular weight leached from frozen yeast increased for longer yeast frozen storage periods. The yeast leachates had an adverse effect on loaf volume.