Congenital orbital cysts of neural tissue in two dogs

A 3-month-old English Cocker Spaniel and a 6-month-old miniature poodle presented with clinical signs related to an abnormal right eye since birth. In both dogs, the right globe could not be identified and was replaced by a fluctuant intraorbital mass covered by a vascularized mucous membrane. Ultrasonography demonstrated that both masses were cystic structures markedly larger in size than the normal contralateral globes. In both cases, surgical excision revealed a multilobular cyst filling the whole orbital cavity. Histopathologic examination and immunostaining for glial fibrillary acid protein and S100 protein supported a diagnosis of neural cysts associated with ocular dysplasia. The definitive diagnosis was congenital cystic eye and microphthalmos with cyst for the Cocker Spaniel and miniature poodle, respectively. Karyotype was normal in both dogs. Congenital cystic eye and microphthalmos with cyst result from defects in early embryonic life that arise following formation of the optic vesicle and prior to closure of the optic fissure. To the authors' knowledge neither has been reported in the canine species. They should be considered in the differential diagnosis of orbital cysts in dogs.     

Survival, neurologic response, and prognostic factors in dogs with pituitary masses treated with radiation therapy and untreated dogs

BACKGROUND: Pituitary masses in dogs are not uncommon tumors that can cause endocrine and neurologic signs and, if left untreated, can decrease life expectancy. HYPOTHESIS: Dogs with pituitary masses that received radiation therapy (RT) have more favorable neurologic outcomes and longer survival times compared with untreated dogs. ANIMALS: Nineteen dogs with a pituitary mass identified on CT or MR imaging were irradiated with 48 Gy given in 3 Gy daily-dose fractions. Twenty-seven untreated control dogs had pituitary masses. METHODS: Medical records of dogs with pituitary masses were retrospectively reviewed for clinical signs, mass size, and outcome. RESULTS: Median survival time was not reached in the treated group. Mean survival time in the treated group was 1,405 days (95% confidence interval [CI], 1,053-1,757 days) with 1-, 2-, and 3-year estimated survival of 93, 87, and 55%, respectively. Median survival in the nonirradiated group was 359 days (95% CI, 48-916 days), with a mean of 551 days (95% CI, 271-829 days). The 1-, 2-, and 3-year estimated survival was 45, 32, and 25%, respectively. Dogs that received RT for their pituitary tumors had significantly longer survival times than untreated dogs (P = .0039). Treated dogs with smaller tumors (based on maximal pituitary-to-brain height ratio or area of tumor to area of brain) lived longer than those with larger tumors (P < .001). CONCLUSIONS AND CLINICAL IMPORTANCE: When compared with untreated dogs, RT increased survival and controlled neurologic signs in dogs with pituitary masses.     

Disposition of plasma creatinine in non-azotaemic and moderately azotaemic cats

The objectives of this study were to compare assay methods for plasma creatinine (Pl-creat) in cats and to describe the disposition of creatinine and iohexol in 12 healthy and moderately azotaemic cats. Exogenous creatinine and iohexol were injected simultaneously by intravenous bolus, and repeated blood samples were taken to determine the pharmacokinetic parameters of each marker. Pl-creat was assayed by high-performance liquid chromatography (HPLC), Jaffé and enzymatic methods. The enzymatic method was shown to be more reliable than the Jaffé method. Two stereoisomers, exo- and endo-iohexol were identified. The plasma clearance of creatinine (2.3+/-0.66 ml/min/kg) was significantly higher (P<0.001) than that of exo-iohexol (1.7+/-0.40 ml/min/kg). The volume of distribution (447+/-97 ml/kg) and elimination half-life (181+/-77 min) of creatinine were also higher (P<0.001) than those of exo- and endo-iohexol. The estimated daily endogenous production of creatinine was 65+/-23 mg/kg. None of the pharmacokinetic parameters was changed by the azotaemic status of the animals.     

Agronomic response and water productivity of almond trees under contrasted deficit irrigation regimes

We investigated the long-term effects of different deficit irrigation (DI) options on tree growth, shoot and leaf attributes, yield determinants and water productivity of almond trees (Prunus dulcis, cv. Marta) grown in a semiarid climate in SE Spain. Three partial root-zone drying (PRD) irrigation treatments encompassing a wide range of water restriction (30%, 50% and 70% of full crop requirements, ET_c) and a regulated deficit irrigation treatment (RDI, at 50% ET_c during kernel-filling) were compared over three consecutive growth seasons (2004–2006) to full irrigation (FI). The results showed that all deficit irrigation treatments have a negative impact on trunk growth parameters. The magnitude of the reduction in trunk growth rate was strongly correlated through a linear relationship with the annual volume of water applied (WA) per tree. Similarly, a significant relationship was found between WA and the increase in crown volume. In contrast, leaf-related attributes and some yield-related parameters (e.g., kernel fraction) were not significantly affected by the irrigation treatments. Except in PRD₇₀, individual kernel weight was significantly reduced in the deficit irrigated treatments. Kernel yield, expressed in percent of the maximum yield observed in the FI treatment, showed a linear decrease with decreasing WA and a slope of 0.43, which implies that a 1% decrease in water application would lead to a reduction of 0.43% in yield. Water productivity increased drastically with the reduction of water application, reaching 123% in the case of PRD₃₀. Overall, our results demonstrate the prevalence of direct and strong links between the intensity of the water restriction under PRD – i.e., the total water supply during the growing season – and the main parameters related to tree growth, yield and water productivity. Noteworthy, the treatments that received similar annual water volumes under contrasted deficit irrigation strategies (i.e., PRD₇₀ and RDI) presented a similar tree performance.     

Intra‐cohort cannibalism in early life stages of pikeperch

Cannibalism has been described in 390 teleost fish species belonging to 104 families. In rearing conditions, intra‐cohort cannibalism is one of the major bottlenecks during the early life stages of pikeperch (Sander lucioperca). This study aims to describe precisely the occurrence and the onset of cannibalism of this species during the first two post‐hatching months. In a first step, the cannibalistic behaviour was analysed through observations in three experiments. In each experiment, the number of cannibalistic cases was similar. From 14 to 48 days post‐hatching (dph), more than 60 % of prey ingestions were realized by tail‐first, but after 48 dph, the number of prey ingestions by head‐first increased. In a second step, the behavioural sequence exhibited to attack and to capture a conspecific was analysed, taking into account the size ratio between the cannibal and its prey. The behavioural sequence to capture was always the same, similar to a predatory sequence and it seems that the cannibal chose its prey about 10 s before attacking. Moreover, prey choice by a cannibal was based more on the size ratio between the cannibal and its prey than the previous activity of the potential prey. These new results could lead to solutions to decrease cannibalism in pikeperch rearing.     

What can we learn by studying dead fish fry?

This study investigated the influence of temperature (4, 8 and 12°C) on development and survival of brown trout (Salmo trutta) fry. The three aims of this study were: (a) to propose a typology of malformations; (b) to compare malformation types between live and dead fry and (c) to establish relationships between temperature and malformation occurrences. It was found 20 single malformations and 39 combinations of two or more malformations. Comparison between dead and live fry at different development stages (hatching, emergence and first food intake) showed that malformations of yolk sac were predominant at hatching and then decreased, while malformations of skeleton or multiple malformations were higher thereafter. All dead fry, and only 14% of live fry were malformed. Dead fry were mainly characterized by yolk sac malformations and multiple malformations whatever the temperature. Live fry showed a higher rate of skeleton malformations at 12°C, and the different types of malformations were equally represented at two other temperatures (4 and 8°C). To conclude, it is suggested that some malformations (yolk sac at hatching, yolk sac associated with skeleton malformations at emergence and skeleton at first food intake or combinations of malformations at all stages)might be lethal as they were founding dead fry and that temperature influences differently the occurrence of malformations.