红外增温对库尔勒香梨越冬期树干形成层主要生理参数的影响

为了探讨越冬期树干形成层温度变化对形成层组织生理参数的影响,以库尔勒香梨为实验材料,在太阳辐射和气温达到日最大值的时间段,利用控温红外灯将实验组树干阳面形成层温度分别增温达到16℃、19℃、22%、25℃、28℃、31℃、34℃,采用热电偶温度传感器同时连续监测形成层温度并结合主要生理参数的变化,分析越冬期间树干形成层温度变化引起的融冻交替对树干组织主要生理参数的影响。结果表明,增温达到最大阳面形成层温度日较差的处理条件下,SOD、POD和CAT活性比对照分别降低了83.60%、84.62%、47.56%,相对电导率和可溶性蛋白含量分别升高了41.46%、46.81%;生理指标和形成层温度均呈负相关,其中CAT、可溶性蛋白和相对电导率呈显著性负相关。形成层温度日较差或融冻交替幅度与各生理参数均呈负相关,其中CAT、可溶性蛋白和相对电导率呈显著性负相关。保护酶系中,SOD对形成层温度变化最为敏感;虽然POD保护作用稍微迟钝,但在较强烈的形成层温度变化的条件下起到最好的保护作用;在形成层温度强烈波动的情况下,CAT保持最小变化幅度;可溶性蛋白通过调节渗透势,减轻细胞伤害程度。     

Cytoplasmic dynein moves through uncoordinated stepping of the AAA+ ring domains

Cytoplasmic dynein is a homodimeric AAA+ motor that transports a multitude of cargos toward the microtubule minus end. How the two catalytic head domains interact and move relative to each other during processive movement is unclear. Here, we tracked the relative positions of both heads with nanometer precision and directly observed the heads moving independently along the microtubule. The heads remained widely separated, and their stepping behavior varied as a function of interhead separation. One active head was sufficient for processive movement, and an active head could drag an inactive partner head forward. Thus, dynein moves processively without interhead coordination, a mechanism fundamentally distinct from the hand-over-hand stepping of kinesin and myosin.     

Modified atmosphere packaging and ethanol vapor to control decay of ‘Red Globe’ table grapes during storage

The aim of this study was to determine the efficacy of several alternative postharvest treatments to sulfur dioxide (SO₂) in maintaining quality and reducing fungal decay during cold storage of ‘Red Globe’ table grapes. Grapes were packaged in perforated polyethylene (PPE) or modified atmosphere packaging (MAP) bags (ZOEpac or Antimicrobial) with or without different grades of ethanol vapor-generating sachets (Antimold^®30, Antimold^®60 or Antimold^®80) or a SO₂-generating pad, and the grapes were kept at 0 °C and 90-95% relative humidity for 4 months. Packaging of grapes with a SO₂ pad in PPE or ZOEpac bags provided better control of fungal decay and stem browning than PPE or ZOEpac bags alone, PPE or ZOEpac bags with Antimold sachets or Antimicrobial bags alone. The PPE bag containing the Antimold^®80 sachet was as effective as the SO₂ treatments in reducing the incidence of fungal decay in naturally infected and artificially inoculated grapes for 1 month. Ethanol vapor released by the Antimold sachets enhanced berry color, but caused stem browning depending on ethanol vapor concentrations in the headspace of the bags. The ZOEpac-210 bags alone resulted in significantly lower weight loss and incidence of stem browning, compared to other treatments; however, they did not reduce fungal decay or develop an appreciable level of modified atmosphere.     

Global incidence of wheat rusts and powdery mildew during 1969–2010 and durability of resistance of winter wheat variety Bezostaya 1

Disease incidence and severity was studied for winter wheat variety Bezostaya 1 and susceptible checks based on data from international nurseries from 1969 to 2010 and from 51 countries across major winter wheat production regions totalling 1,047 reports. The frequency of leaf rust and stripe rust occurrence was stable over time with marked increases in severity in 2001–2010 especially in Europe and Central and West Asia. Substantial global reductions in stem rust occurrence were recorded and attributed primarily to use of resistance genes although the recent emergence of race Ug99 makes wheat more vulnerable. The occurrence of powdery mildew remained globally stable over time. It was the most important foliar disease in Western and Southern Europe, where the frequency was very high for all time periods coupled with slight increases in severity during 2001–2010. The durable resistance of variety Bezostaya 1 to all four diseases was demonstrated in the study using comparisons of disease severities of Bezostaya 1 and the most susceptible entries. The Lr34/Yr18/Pm38 pleiotrophic set possessed by Bezostaya 1 is currently an important target for selection because it is now amenable to molecular selection. Increased use of genes like Lr34 combined with strategies to minimize cultivation of extremely susceptible varieties will contribute to long term maintenance of low and non-damaging disease levels. The durable disease resistance of Bezostaya 1, combined with its adaptability and good end-use quality, was a significant reason for its huge impact in agriculture over the last 50?years.     

Kinesin walks hand-over-hand

Kinesin is a processive motor that takes 8.3-nm center-of-mass steps along microtubules for each adenosine triphosphate hydrolyzed. Whether kinesin moves by a "hand-over-hand" or an "inchworm" model has been controversial. We have labeled a single head of the kinesin dimer with a Cy3 fluorophore and localized the position of the dye to within 2 nm before and after a step. We observed that single kinesin heads take steps of 17.3 +/- 3.3 nm. A kinetic analysis of the dwell times between steps shows that the 17-nm steps alternate with 0-nm steps. These results strongly support a hand-over-hand mechanism, and not an inchworm mechanism. In addition, our results suggest that kinesin is bound by both heads to the microtubule while it waits for adenosine triphosphate in between steps.     

Comparison of the Salt Stress Tolerance of Promising Turkish Winter Squash (Cucurbita maxima Duch.) and Pumpkin (Cucurbita moschata Duch.) Lines and Interspecific Hybrids

The objective of this study was to evaluate several selected Cucurbita genotypes for their salt resistance in a rootstock breeding program for grafted watermelon seedling production. Specifically, changes in the relative water content (RWC), leaf area (LA), total chlorophyll content (TCC) and proline concentration (PC) of a commercial cultivar (G32 code), two promising winter squash (G12 and G13 of Cucurbita maxima Duch.) lines, six pumpkin (G3, G27, G28, G29, G30 and G31 of Cucurbita moschata Duch.) lines, and four C. maxima?×?C. mochata hybrids (G14, G15, G40 and G42), were investigated with a control treatment and four levels of salt stress (4, 8, 12 and 16?dS/m). In this study, Cucurbita cultivars which showed significant differences according to RWC, LA, TCC and PC at least significant difference (LSD) tests (P?<?0.05) and had salinity sensitivity index (SSI) value lower than 20%, were assessed as salt tolerant genotypes. The salt tolerant genotypes were grouped with principal component analysis (PCA) in each salt level. At the end of the study, all genotypes for all characteristics were generally tolerant at 4?dS/m salinity level. The G3, G12, G13, G14, G29 and G42 genotypes were resistant at 8?dS/m salinity, while the G15, G31, G32 and G40 were the most resistant genotypes at 12 and 16?dS/m. In conclusion, the selection of more salt resistant cultivars in rootstock breeding programs should be a priority to maintain growth performance in saline environments.

     

Determination of the chemical composition and antioxidant activity of the essential oil of Artemisia dracunculus and of the antifungal and antibacterial activities of Turkish Artemisia absinthium, A. dracunculus, Artemisia santonicum, and Artemisia spicigera essential oils

The essential oil isolated from Turkish tarragon (Artemisia dracunculus) by hydrodistillation was analyzed by GC-MS. Thirty compounds representing 99.5% of total oil were identified. The predominant components in the oil were (Z)-anethole (81.0%), (Z)-beta-ocimene (6.5%), (E)-beta-ocimene (3.1%), limonene (3.1%), and methyleugenol (1.8%). The antibacterial and antifungal activities of the essential oils isolated from A. dracunculus, Artemisia absinthium, Artemisia santonicum, and Artemisia spicigera oils were also evaluated. In general, the oils exhibited potent antifungal activity at a wide spectrum on the growth of agricultural pathogenic fungi. Among the oils, the weakest antifungal activity was shown by the oil of A. dracunculus. In many cases, the oils of A. absinthium, A. santonicum, and A. spicigera completely inhibited the growth of some fungal species. As compared with antibacterial activities of all of tested oils, A. santonicum and A. spicigera oils showed antibacterial activities over a very wide spectrum. However, the essential oils tested showed lower inhibition zones than the inhibition zones of penicillin. In addition, antioxidant and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities of tarragon oil were determined, and weak antioxidant and DPPH radical scavenging activities were found in comparison to butylated hydroxytoluene.     

Response of red pepper to deficit irrigation and nitrogen fertigation

The main objective of this investigation was to evaluate the response of red pepper grown in a subhumid climate to different irrigation and nitrogen levels. Open-field trials were conducted in the Marmara Region, Turkey. Plants were subjected to three water levels [full irrigation (FI) = 100% crop evapotranspiration (ETc) and two deficit irrigations (DIs)= 66 and 33% ETc restoration] and four levels of N (0, 80, 160, and 240 kg N ha^?1) during the 2012, 2013, and 2014 growing seasons. A split-plot experimental design was used. The highest values of biomass and marketable yield (MY) were observed under FI. DI significantly increased the fruit soluble solids content. The biomass yield, MY, and fruit weight significantly improved with increasing nitrogen levels. The 240 kg N ha^?1 treatment under FI provided the maximum net income. Increasing N supply under DI conditions enhanced the water-use efficiency based on both biomass yield and MY. These results indicate that with respect to the yield, the net income, and the water productivity of red pepper, the FI with a nitrogen supply of 160–240 kg ha^?1 is recommended for drip irrigated and N-fertigated red pepper under subhumid climate conditions.