Generalized aspergillosis in dairy sheep

Two cases of systemic aspergillosis are described in dairy sheep from a flock in which fungal mastitis appeared subsequent to the antibiotic treatment of animals before parturition. Lesions characterized by necrosis and a pyogranulomatous exudate were observed in the mammary glands, supramammary and mediastinal lymph nodes, kidneys, lung, liver, heart, forestomachs and brain. The intense vasculitis with thrombosis observed in various organs, but especially in the mammary glands, suggested a haematogenous dissemination of the infection from this organ. The aetiological diagnosis was accomplished by the immunohistochemical staining of the fungal structures seen in the histological sections by the specific Aspergillus monoclonal antibody Mab-WF-AF-1 together with the isolation of Aspergillus fumigatus in pure culture from affected tissues.     

大规格优质鲫网箱养殖高产技术总结

为了缩短网箱养殖优质鲫的周期,简化生产流程,降低生产成本,提高经济效益,2003年我们在岸堤水库进行大规格优质鲫网箱养殖试验,取得了成功。在此基础上,2004年我们依托技术优势,组织部分养鱼户,新上大规格优质鲫网箱96箱,并牵头与外地客商签订供销合同,商品鱼全部出口韩国,当年商品鱼总产量达432t,实现收入480多万元。现将2组（共6只）网箱生产管理技术要点总结如下：     

PRRSV GP5蛋白抗独特型单链抗体的构建表达及鉴定

为制备只包含抗体可变区片段(Fv)的MAb2-5G2单链抗体(scFv),本研究从分泌MAb2-5G2的杂交瘤细胞中提取总RNA,反转录制备cDNA作为模板扩增重链可变区(VH)和轻链可变区(VL)基因.以VH-1inker-VL的形式通过重叠PCR制备scFv基因,构建重组表达质粒pEasy-scFv,并在大肠杆菌中获得正确表达.通过western blot和间接免疫荧光鉴定表明scFv蛋白能够被兔抗MAb2-5G2的抗体(Ab3)识别,复性后的scFv蛋白能够特异性结合Marc-145细胞.该研究结果为进一步明确MAb2-5G2结合Marc-145细胞功能区域奠定了物质基础.     

猪波形蛋白对PRRSV感染Marc-145细胞的抑制作用

为了研究波形蛋白在介导猪繁殖与呼吸综合征病毒(PRRSV)感染细胞过程中的作用,利用RT-PCR方法从PRRSV非易感细胞系猪肾细胞系PK-15细胞中扩增目的基因,克隆入pET-28a(+)载体,转化大肠杆菌BL21(DE3)中进行诱导表达。表达的重组猪波形蛋白经SDS-PAGE和Western blot鉴定和纯化后免疫新西兰大白兔制备多克隆抗体。利用病毒抑制试验检测重组猪波形蛋白及多克隆抗体在PRRSV感染Marc-145细胞过程中的作用。结果表明,成功扩增猪波形蛋白基因并克隆入pET-28a载体,经诱导后得到高效表达,纯化后免疫兔子产生高价血清抗体(105)。病毒阻断结果表明,猪波形蛋白及多克隆抗体均能阻断PRRSV感染Marc-145细胞。这为以波形蛋白为基础的PRRSV受体阻断抑制剂的研究提供了实验依据。