THE PATHOGENESIS OF INFECTIOUS AVIAN ENCEPHALOMYELITIS

Infectious avian encephalomyelitis virus (IAEV) maternal antibody was detected in the serum of chickens for up to 21 days following hatching. This antibody protected chickens against clinical IAE after intracerebral inoculation with van Roekel strain or oral administration of the NSW-1 strain of IAEV. Maternal antibody to IAEV also protected testosterone bursectomised chickens against the development of clinical disease. IAEV maternal antibody also influeced the pattern of virus excretion in faeces and serological responsiveness. This influence on antibody responses persisted beyond the time that IAEV maternal antibody could be detected. The importance of IAEV maternal antibody on the strategy of vaccination against IAE is discussed.     

Microbiology of normal and seborrheic canine skin.

Quantitative and qualitative bacterial assays were performed on the skin of 15 normal and 32 seborrheic dogs. Nonionic detergent scrubs were made on areas demarcated by glass sidearm cylinders. Quantitative analysis was accomplished by the serial dilution technique, and the bacteria were identified by individual and colonial morphology and by enzyme production. Areas measured on control dogs had a markedly lower total bacterial count than similar areas measured on seborrheic animals. Control dogs had a flora consisting primarily of coagulase-negative cocci, whereas seborrheic dogs usually had a cutaneous flora composed primarily of Stahylococcus aureus, coagulase-positive.     

Parainfluenza-3 virus exposure of beef heifers prior to natural breeding.

Ninety-six Hereford heifers (approximately 7 months of age) were randomly divided into 2 equal groups and housed 1.6 km apart (with 2 replications in time, 1 year apart). At 15 months of age, 1 group/replicate was inoculated with parainfluenza-3 virus, and the other group was given virus-free spent culture medium. Twenty-four hours later, 2 virgin bulls (2 years old) were placed with each group (24 cows) for natural breeding. Viral inoculation caused a twofold increase in parainfluenza-3 titer and a 0.3 C body temperature increase. There was no effect recognized from the virus on natural breeding efficiency.     

Cross reactions of normal bovine sera with foot-and-mouth disease virus: incidence, duration, and effect of shipping stress.

Serum samples were obtained from 30 Hereford steers in an area known to be free of foot-and-mouth disease (FMD) viruses as follows: before shipment and 4 times during a 70-day period after shipment; the sera were tested for the presence of cross-reacting antibody to various viruses. Percentages of sera containing cross-reacting antibody to FMD virus detected by the plaque-reduction neutralization and the radial immunodiffusion techniques were higher for the FMD viruses Asia and SAT I5 than for the FMD viruses A5, O1, and C1. Cross-reacting antibody was usually of low titer and usually present in only 1 or 2 consecutive serum samples. The incidence of cross reactions increased after stress of shipping and thus an infective agent may be responsible. These results were compared with results from sera collected from Herefords and Holstein-Friesians in a 2nd area; results did not indicate that Herefords have an excess of cross reactions with FMD viruses.     

The performance of cross‐bred hens given free choice feeding of whole grains and a concentrate mixture and the influence of source of xanthophylls on yolk colour

1. Cross‐bred hens were offered, from 25 to 73 weeks of age, diets based on either wheat or barley in the form of either a mash or as whole grains plus a concentrate mixture; four sources of xanthophylls were also compared.

2. Wheat reduced food intake by 8%, increased efficiency of food conversion and live‐weight gain while barley increased litter moisture content, and, during the first half of the laying year decreased egg production by 3.3% and yolk colour by 4%.

3. Hens offered whole grains ate 11% less food, converted food into eggs more efficiently, were heavier and laid larger eggs than those given the mash diets.

4. With whole grains yolk colour was less than with mash diets during the first 24 weeks of lay but this was reversed during the last 24 weeks of lay.

5. Either canthaxanthin or citranaxanthin or β‐apo‐8'‐carotenoic acid ethyl ester plus canthaxanthin in diets containing lucerne meal produced eggs with acceptable yolk colours.     

Studies on the avian shell gland during egg formation: The effect of acetazol‐amide on the transfer of ions to the albumen 1

1. Twenty‐five laying hens were fed on a diet containing 100 mg acetazolamide/kg in order to determine the effects of a decrease in the activity of carbonic anhydrase on the transfer of minerals to the egg albumen.

2. Treatment with acetazolamide decreased the rate of shell formation by 44%; reduced the concentrations of water and Na+ in the albumen at the beginning of the plumping stage but increased the accumulation of water during plumping; increased the concentration of Cl‐ in the albumen after the 6‐h stage without any appreciable change in K+ and Ca²⁺ concentrations.

3. The computed relationships between the concentrations of different ions also showed that the transfer of water and Na+ were linked during egg formation, that a water‐independent, acetazolamide‐sensitive reabsorption of Na+ occurred after the 10‐h stage and that Na+ and Cl‐moved simultaneously up to 14 h but with the ratio of Cl‐ to Na+ three times higher in the treated group.

4. It is concluded that acetazolamide impairs the transfer of Na+ and Cl‐ between the albumen and the extracellular fluid and that secretion of Ca²⁺ into the uterine lumen seems to depend on Na+ and Cl‐ reabsorption.     

Effects of age on the tryptophan requirement of laying hens

1. White Leghorn pullets which had been used for an assay of tryptophan requirement between 32 and 40 weeks of age were used for similar determinations between 63 and 73 and, after a moult, from 97 to 106 weeks of age.

2. A tryptophan‐limiting protein mixture was used and by dilution seven dietary protein contents were produced, supplying from 0.84 to 1–92 g tryptophan/kg diet. The diet of lowest protein content was also sup‐lemented with free tryptophan. These diets were fed in experiments using 24 groups of 72 pullets at 63 to 73 weeks and 45 groups of 21 hens at 97 to 106 weeks.

3. The relationship between egg output and tryptophan intake was the same in moulted hens as in young pullets, but pullets of 63 to 73 weeks of age yielded a different response curve; more tryptophan being needed for a given egg output.

4. It is concluded that tryptophan required, per day, does not decrease during the first laying year, despite a decrease in rate of egg output.     

Stability of abdominal fat and meat of broilers: Effect of duration of feeding antioxidants 1

1. The α‐tocopherol (AT) content of abdominal fat and fat stability increased as a function of the duration of α‐tocopheryl acetate (ATA) feeding in broilers fed on diets containing either 40 g acidulated soyabean‐oil soapstock (ASS) or tallow/kg. A linear relationship was observed between AT content of the adipose tissue and its stability with both types of fat supplements.

2. The stabilities of carcass fat and meat of 9‐week‐old broilers fed on diets containing ASS with 300 mg ethoxyquin (EQ)/kg or diets containing tallow with 20 mg ATA/kg from weeks 5 to 9 were comparable with those obtained by feeding these antioxidants and fats throughout the growth period.

3. ATA added to a diet containing ASS, and EQ, added to a diet containing tallow did not improve meat stability despite such an effect on adipose tissue when fed for 9 weeks. The results emphasise the importance of using the dietary antioxidant appropriate to the fat supplement to improve carcass stability.     

The photoperiodic effect of ahemeral light‐dark cycles which entrain circadian rhythms

1. A 27‐h cycle of light and dark provides a greater gonadotrophic stimulus to the laying fowl, as judged by sexual maturity and rate of lay, than a 24‐h cycle incorporating the same photoperiod.

2. An hypothesis put forward to account for these effects states that Effective Photoperiod equals p + c – b, where p = actual photoperiod, c = cycle length and b = the period of the endogenous biological clock.

3. Two experiments designed to test this hypothesis have yielded results which are consistent with it.

4. A poultryman who uses an ahemeral cycle to alter egg weight or shell thickness and then wishes to transfer his flock back to a 24‐h cycle should calculate the difference between the two cycle lengths and then add this quantity to the prevailing photoperiod to find the appropriate amount of light to be used in the 24‐h cycle.     

The action of sulphanilamide on shell deposition

1. The action of sulphanilamide on egg shell deposition has been studied in the domestic fowl. The length of time that eggs remain in the shell gland was measured; the relative thickness and protoporphyrin pigment concentration for egg shells removed from the shell gland were determined; egg shell structure was examined using a replica technique.

2. Sulphanilamide appeared to act mainly by reducing the rate of shell deposition, time spent in the shell gland was unaffected.

3. The thickness of shell components was reduced in a roughly proportional manner in thin shells produced during sulphanilamide treatment.

4. It is proposed that other thin shells in which the component layers are all reduced to a similar extent are also the result of a decreased deposition rate.