永嘉县龙湾潭国家森林公园建设问题思考

以永嘉龙湾潭国家森林公园为例,分析了我国森林公园规划、建筑和基础设施、文化内涵、旅游服务和整体营销等方面存在的问题,提出了永嘉县龙湾潭国家森林公园在特色森林公园建设中就用浓厚的地方特色建筑、加强人文景观与自然景观结合、加强森林公园整体营销策划等建议.     

Analysis of Original Causes of Reed Fires in Zhalong Nature Wetland Reserve in Heilongjiang Province

Zhalong Nature Reserve is located in the border areaof Qiqihaer City, Fuyu County, Lindian County andTailai County of Heilongjiang Province. The area ofthe reserve is 210 000 ha. The reserve is the firstnature reserve for protecting wetland and crane inChina. There were more than 300 red-crownedcranes here at the very most. Zhalong Nature Reserveis situated at the Songnen Plain, the lower stream ofthe Wuyuer River. It is the biggest nature reserve inChina that takes crane and wetland …     

牛心朴子内生真菌分离及其抑菌活性分析

为了解荒漠植物牛心朴子(Cynanchum komarovii)内生真菌多样性及其抑菌活性特征,通过组织块法分离其内生真菌,并选择5种植物病原真菌和4种人体病原细菌作为指示菌测定抑菌活性。结果表明:分离的25株内生真菌以茎部最多,根部次之,叶部最少,经形态学初步鉴定归于2目3科11属,曲霉属(Aspergillus)为优势菌属;84%的分离菌株对2种以上病原真菌,以及60%的分离菌株对1种以上病原细菌有不同程度的拮抗作用,2株高活性菌株CKEFL003和CKEFS001对多种病原菌拮抗作用明显,均为曲霉属。牛心朴子内生真菌在根茎叶组织器官中数量分布存在差异,种群构成具有多样性特征,对植物和人体病原菌具有不同程度的拮抗作用,抑菌资源较为丰富,高活性菌株为曲霉属,抗菌谱较宽。     

Alteration of glutathione S-transferase properties during the development of <Emphasis Type="Italic">Micromelalopha troglodyta</Emphasis> larvae (Lepidoptera: Notodontidae)

Micromelalopha troglodyta (Graeser) is an important pest of poplar in China. Glutathione S-transferases (GSTs) are known to be responsible for adaptation mechanisms of M. troglodyta. The activities and kinetic constants of glutathione S-transferases in M. troglodyta were studied. Significant differences in glutathione S-transferase activity and kinetic characteristics were observed among five instars of M. troglodyta larvae. Furthermore, the inhibition of glutathione S-transferase activity in five instars by 24 inhibitors was conducted. The results show the inhibition of GST activity of different instars by 24 inhibitors was different. For GST activity in the 1st instar, chlorpyrifos, lambda-cyhalothrin, endosulfan, abamectin, fipronil and pyridaben were the best inhibitors tested, and for GST activity in the 2nd instar, tannic acid and quercetin were the most potent inhibitors tested, and for GST activity in the 3rd instar, the inhibitory effects of quercetin, chlorpyrifos and lambda-cyhalothrin were the highest, and for GST activity in the 4th instar, quercetin and lambda-cyhalothrin were the best inhibitors, and the inhibitory effect of phoxim was the highest for GST activity in the 5th instar. Our results show that glutathione S-transferases in different instars are qualitatively different in isozyme composition and thus different in sensitivity to inhibitors.     

几种害虫夜间上灯节律分析与应用研究

对非洲蝼蛄(Gryllotalpa africana PMisot de Beauvios)、暗黑金龟(Holotrichia padlela Motschulsky)和铜绿金龟(Apomala corpulenta Motschulsky)成虫在3种不同诱虫灯下的夜间上灯节律进行了研究。表明在3种不同灯下，上述3种害虫的上灯节律基本趋于一致，全夜各有1个上灯高峰，均在开灯后1h内，上灯比例分别为全夜的37．39％、40．49％和48．20％。进行综合各灯种逐时段的上灯累积百分率分析，发现该3种害虫到凌晨1：00止，累积上灯百分率分别为全夜诱虫量的88．69％、88．30％和85．63％，据此，可以结合田间实际虫情和防治要求，将凌晨1：00确定为最佳关灯时间，以节约诱虫成本。     

大蒜高产栽培技术

大蒜具有杀菌、止痒等功能，其香味独特，适口性较强。因此，深受人们的喜爱。因其栽培方法简单易行，且产量较高，经济效益较好，所以阜阳市颖州区栽培面积有逐年扩大的趋势。现就其栽培方法简要介绍如下：     

大豆营元对HEC-1B细胞增殖及雌激素受体 报告基因表达的影响

〔摘要〕目的探讨大豆葺元对子宫内膜癌细胞增殖的影响及其对雌激素受体报告基因表达的影响、方法体 外培养子宫内膜癌细胞子宫内膜癌雌、孕激素受体阴性细胞系(HEC 1B),采用四甲基偶氮哩盐法(M1"P)检测不同 浓度大豆葺元对子宫内膜癌细胞增殖的影响,同时检测大豆葺元对雌激素应答原件(ERE)调控的雌激素受体。 (ERa)和雌激素受体(3 ( ER(3)的荧光素酶报告基因表达的影响〔结果大豆葺元对HEC-1B细胞体外增殖有明显 抑制作用,差异有统计学意义(P<0.01),其抑制作用有明显量效和时效性特征〔在浓度为20-80x 1 O}mol/L的大豆葺 元作用卜,报告基因1u。的表达较正常对照组明显提高,差异有统计学意义(P<0.05 ),在80x 10-`mol/L时到达最高 值;大豆葺元通过ER日介导的报告基因表达水平的升高程度强于ERa,差异有统计学意义(P<0.01)〔结论大豆葺 元可通过调节子宫内膜癌细胞ER。和ER日介导的报告基因的表达,调整ERa/ER日比例,从而抑制子宫内膜癌细 胞的增殖、     

不同硬粒小麦品种的单粒种子PCR分析鉴别法

用PCR(聚合酶链式反应)法扩增来源于几个硬粒小麦单粒萌发种子DNA提取液中的LMW(低分子量)谷蛋白基因序列.PCR反应的电泳分析表明,品质优劣不同的硬拉小麦品种具有不同的扩增产品.这种建立在PCR基础上的方法,比标准方法筛选优异品质基因型更有效、更安全.     

不同肥力桑田供N指标值探讨

几年来肥料试验结果证明,桑田合理配施三要素肥,不仅能提高桑叶产量和质量,而且具有提高肥料利用率和对土壤养分消长及养分平衡等方面作用。了解桑田土壤供 N能力是做到“以田定产,以产定 N.以 N 配施 P.K 肥”的前提,实际上影响土壤供 N 量的因素很多,但前人对桑田土壤供 N 量最适测定项目及其数量指标的研究极少报道,为此,我们在结合“桑田配施三要素肥料研究”中间试验过程中,对我省有代表性蚕桑区(八县一场23个农户)不同类型和不同肥力桑田土壤进行全面理化分析及产量试验调查,并以国内外广泛应用的作物对肥料效应方程研究法,进行分析,取得一些倾向性的