早期不明原因流产患者氧化应激指标与微炎症状态的相关性

目的:检测早期不明原因自然流产患者氧化应激产物和机体的微炎症状态,探讨不明原因自然流产的发病机制.方法:以50例早期不明原因自然流产患者为研究组,50例同期正常早孕要求人工流产的妇女(正常早孕组)和20例健康妇女(健康非孕组)为对照组.检测血浆TNF-α、hs-CRP、IL-6等炎症反应产物和8-isoprostane、MDA、ox-LDL等氧化应激产物的浓度.结果:研究组血浆8-isoprostane、ox-LDL的浓度分别为(148.27±39.35)pg/ml、(689.15±152.06)μg/L,均高于对照组(正常早孕妇女和健康非孕妇女),差异有统计学意义;血浆MDA为(5.13±0.12)μmol/L,研究组也高于对照组,但差异无统计学意义(P＞0.05).研究组TNF-α、hs-CRP、IL-6的浓度分别为(23.53±11.35)pg/ml、(3.84±1.26)mg/L、(28.72±17.38)pg/ml,均高于正常早孕组[(11.45±6.82)pg/ml、(1.82±1.25)mg/L、(16.79±11.19)pg/ml,P＜0.05、P＜0.01、P＜0.05]和健康非孕组[(8.86±1.39)pg/ml、(1.46±1.02)mg/L、(13.35±4.01)pg/ml,P＜0.05、P＜0.01、P＜0.01];正常早孕组也稍高于健康非孕组,但两组差异无统计学意义(P＞0.05).早孕流产患者8-isoprostane及ox-LDL均与hs-CRP、IL-6、TNF-α呈显著正相关(P＜0.01、P＜0.05),MDA与hs-CRP、IL-6、TNF-α无相关性(P＞0.05).结论:正常妊娠存在炎症反应和氧化应激,氧化应激失衡和微炎症状态的加剧及两者的相互作用可能在早期不明原因自然流产中发挥重要作用.干预氧化应激及炎症反应可能有利于控制早期不明原因流产的发生和发展.     

Metamorphosis to Postlarvae of Penaeus chinensis Osbeck by Removal of Heavy Metals from Rearing Systems with Polymeric Absorbent

The effect of removing heavy metal ions (copper, zinc, lead, and cadmium) with a polymeric heavy metal absorbent (PHMA) on metamorphosis of Penueus chinensis Osbeck was investigated. There was a significant ( P < 0.05) reduction in metamorphosis of protozoea to mysis at concentrations more than 31.0 μg/L copper, 38.3 μg/L zinc, 32.5 μg/L lead, and 30.3 μg/L cadmium, in the absence of PHMA. At concentrations more than 61.0 μg /L copper, 68.3 μg/L zinc, 62.5 μg/L lead, and 60.3 μg/L cadmium there was a significant ( P < 0.01) interaction between the absence and the presence of PHMA for metamorphosis of mysis to postlarvae. At concentrations of 12.3 μg/L copper, 45.8 μg/L zinc, 106.2 μg/L lead, and 200.2 μg/L cadmium, the metamorphosis of protozoea to mysis was blocked, but it was 51.4% in the presence of PHMA. At concentrations of 252.3 μg/L copper, 335.8 μ/L zinc, 806.2 μg/L lead and 300.2 μg/L cadmium, the metamorphosis of mysis to postlarvae was stopped, but it was 51.0% in the presence of PHMA. The toxicity of copper, zinc, lead, and cadmium to P. chinensis was reduced with PHMA.     

水溶性有机还原物质研究: Ⅲ. 稻草分解产物的电化学性质及其与可变电荷土壤的相互作用

Some electrochemical properties,such as pH,Eh,and voltammetric behavior.of the decomposition products of rice straw and the interactions of these products with soils were studied.The pH,Eh,and amounts of organic reducing substances changed markedly during the 6-day anaerobic decomposition.pH decreased sharply to pH 5 on the tenth day and then increased gradually to 7 on the 45rh day.The amounts of organic reducing substances increased almost synchronously with the fall of redox potential during the first 15 days.The differential pulse voltammetric(dpv) behavior changed not only in the peak current but also in the peak potential.The fractions with apparent molecular weights lower than 200 daltons appeared to be active in dpv behavior.The electric charge and positively charged,respectively.The group with a low apparent molecular weight and a negative charge was the main components responsible for the lower anodicpeak potentials.They were oxidized first during the interactions of the organic reducing substances with soils.     

淹水后可变电荷土壤铁锰形态的转化

Ectomycorrhizal fungus Laccaria bicolor S238N,isolated from a forest soil in central France in 1990s,has demonstrated unequivocally and ability to promote pine growth.In the present nursery bed experiment,the ability of this ectomycorrhizal fungus to increase growth and P and K uptake of Douglas Fir seedlings (Zone 22) was examined.Growth of inoculated seedlings was over twice(plant height) and three times (biomass)that of non-inoculated ones.Similarly,both the concentrations and the amounts of P and K uptake by seedlings were significantly increased by fungal inoculation,indicating the improvement of P and K nutrition in mycorrhizal seedlings.In contrast,Al-P in the soils was decreased obviously by plants,especially by mycorrhizas,suggesting utilization of this soil P pool by plants and more efficient Al-P mobilization by mycorrhizas than by nomycorrhizas.Moreover,K extracted by 1mol/L HCl following consecutive extraction of H2O and CH3COONH4,which may not be plant available,could be utilized by fungus colonied roots.This could be explained by the release of protons and oxalate by hypae which leads to replacement of interlayer K in nonexpanded 2:1 clay minerals and bio-weathering of phyllosilicates.