滇中冷凉山区不同播期反季花椰菜产量效益分析

为摸索滇中高海拔冷凉山区反季节栽培花椰菜的最佳播期以集成高效栽培技术推广应用,于2017—2018年选择海拔2250 m的云南省峨山县塔甸镇大西村地块进行9个播期的2年随机区组试验。结果表明,花椰菜生育期随播期推迟而延长,而花球采收期除播期7月10日外随播期推迟而逐渐增长;花椰菜株高、外叶数、开展度、球高、球径和单球重等农艺性状有随播期延迟呈现先逐渐减小而后又逐渐增大的趋势;莲座期黑腐病和霜霉病的病情指数随着播期的延迟呈现先逐渐升高而后又逐渐下降的趋势;花椰菜小区产量随着播期的延迟呈现先逐渐下降而后又逐渐提高的趋势,播期4月20日和4月30日与其余7个播期产量之间的差异达极显著水平。综合花椰菜在冷凉山区反季节栽培的生产实际和各播期产量产值及商品性表现,推荐滇中高海拔冷凉山区反季节栽培花椰菜的最佳播期为4月20—30日。     

北京市丰台区10kV配电线路故障分析

首先分析了北京市丰台区的电力负荷特点及设备情况,然后利用丰台区2019年的配电线路电力故障数据,分别以故障地点和故障原因两方面进行归类和分析,提出相应的防护措施,为提升丰台配电网的可靠性和保障配电网的安全运行提供了依据。     

5种生物制剂对马铃薯种薯萌芽、极端天气下植株生长和产量性状的影响

旨在满足马铃薯生产中茬口衔接、机械化生产技术应用、不利气候下稳产等对马铃薯出苗早、齐、壮的需求,以‘费乌瑞它’为供试品种,用基于有益活菌或工程菌提取物的5种生物制剂进行种薯处理,对多重性状进行了对比分析。5种生物制剂较常规化学制剂,均能够不同程度地促进种薯萌芽和芽根同生,出苗期提前2~7天,播种后49天的出苗率提高3.33%~17.78%。其中,表现最好的为酵母核苷酸衍生物和VDAL,种薯萌发和生根均显著高于对照。霜冻后,生物剂拌种处理在恢复前期促进植株生长,由此促进恢复后期的块茎发育,较常规化学处理增产8.39%~24.03%,体现了不同程度的保产效果。多马道黑、酵母核苷酸衍生物、根肽和VDAL体现出较好的保产效果,可作为种薯处理剂投入马铃薯生产。     

围场县马铃薯中典型药物污染特征及健康风险评价

为掌握典型药物在农作物中的污染特征及健康风险,保障农产品质量安全,利用超声萃取-固相萃取-高效液相色谱/串联质谱(LC-MS/MS)技术,对围场县全县马铃薯中5大类(磺胺类、四环素类、氟喹诺酮类、大环内酯类及其他类)25种药物污染水平及富集效果进行了调查,并对健康风险进行了评价。结果表明,有20种目标物被检出,不同目标物检出率差异显著,检出含量范围为0.13～3.67μg·kg-1,单种污染物最高检出含量为17.0μg·kg-1。马铃薯对目标污染物的富集系数范围在0.01～39.6之间,最大富集系数为61.8。畜禽粪便是马铃薯中药物残留的主要来源,尽管整体检出水平和健康风险相对较低,但长期摄入与协同作用引发的潜在健康风险不容忽视。因此,仍需严控污染源头,推进畜禽粪便安全资源化利用,确保农田环境质量及农产品安全品质。     

Effect of chlorogenic acid on intestinal inflammation,antioxidant status,and microbial community of young hens challenged with acute heat stress

Heat stress in poultry is deleterious to productive performance. Chlorogenic acid (CGA) exerts antibacterial, anti-inflammatory, and antioxidant properties. This study was conducted to evaluate the effects of dietary supplemental CGA on the intestinal health and cecal microbiota composition of young hens challenged with acute heat stress. 100-day-old Hy-line brown pullets were randomly divided into four groups. The control group (C) and heat stress group (HS) received a basal diet. HS + CGA₃₀₀ group and HS + CGA₆₀₀ group received a basal diet supplemented with 300- and 600-mg/kg CGA, respectively, for 2 weeks before heat stress exposure. Pullets of HS, HS + CGA₃₀₀, and HS + CGA₆₀₀ group were exposed to 38°C for 4 h while the control group was maintained at 25°C. In this study, dietary CGA supplementation had effect on mitigate the decreased T-AOC and T-SOD activities and the increasing of IL-1β and TNFα induced by acute heat stress. Dietary supplementation with 600 mg/kg CGA had better effect on increasing the relative abundance of beneficial bacterial genera, such as Rikenellaceae RC9_gut_group, Ruminococcaceae UCG-005, and Christensenellaceae R-7_group, and deceasing bacteria genera involved in inflammation, such as Sutterella species. Therefore, CGA can ameliorate acute heat stress damage through suppressing inflammation and improved antioxidant capacity and cecal microbiota composition.     

Effects of dietary vitamin K levels on bone quality in broilers.

This study was conducted to evaluate the effects of dietary vitamin K (menadione) on bone quality in cage-raised broilers. Three hundred and sixty male broilers were randomly allotted to one of six treatments, with six replicate pens per treatment and 10 chicks per pen. Broilers were fed one of six diets including a control diet or the control diet plus graded levels of vitamin K (0.5 mg/kg, 2 mg/kg, 8 mg/kg, 32 mg/kg and 128 mg/kg). Water and feed were provided ad libitum during the 7-week experimental period. Results indicated that vitamin K supplementation of broilers diets significantly effected bone quality and feed efficiency. The treatment containing vitamin K at 8 mg/kg improved growth performance (during weeks 6-7) and bone quality (during weeks 0-3). In our study, hydroxyapatite binding capacity of serum osteocalcin (during weeks 0-3), bone breaking strength, bone flexibility, bone ash weight increased linearly (P < 0.05) and bone mineral density, bone mineral content increased quadratically (P < 0.05) with increasing supplementation of vitamin K. In conclusion, to gain optimum bone quality and broiler performance, our studies suggest that the concentration of vitamin K in broilers diets should be 8 mg/kg, 2 mg/kg, and 2 mg/kg, for the starter, grower and finisher phases, respectively. Furthermore, it was shown that the starter period is an important phase for improving bone quality. In addition, this study validated the mechanism of vitamin K effects on bone quality. Vitamin K boosts the carboxylation of osteocalcin and decreases the concentration of serum under-carboxylated osteocalcin enhancing hydroxyapatite binding capacity of serum osteocalcin and improving bone quality.     

S1核酸酶突变检测法的优化

优化了S1核酸酶突变检测体系,结果表明(1)扩增法比杂交法产生异源双链DNA更节省时间;(2)PCR产物可以直接作为突变检测的底物;(3)适当降低反应温度、适当增加反应缓冲液中的NaCl浓度、适当缩短反应时间可提高突变检测效果。     

细叶桉不同立地种源试验

应用细叶桉２５个地理种源进行了２批不同立地种源试验，对４－６年生幼林生长表现和适应性，运用指数分析法进行评价，结果表明：强轩桉种源间各怀状差异，山地片达到显著或极显著水平，平地片除第２批材积差异显著外，其余均不显著。     

合成二氢月桂烯醇的研究进展

综述了从蒎炮合成二氢月挂烯醇的路线及关键反应的研究进展，包括蒎烯氢化、蒎 烷异构化。一步法和二步法从二氢月桂烯制二氢月桂烯醇。     

膨化水产饲料的密度控制技术

鱼类的消化道比较短,而且口腔摄取食物变化也不同于家畜,加上生活在水环境中,因此要求饲料必须易消化而且形状、大小和水中的沉浮性必须与鱼的大小、种类、自然采食习惯相适应。可以通过配方和加工工艺控制饲料的这些特点。这些指标中相比较水产饲料的密度被认为是评价产品质量