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81.
中药“增免散”对免疫抑制鸡免疫功能的影响 总被引:5,自引:1,他引:5
将300只1日龄雏鸡随机分成正常对照组、中药“增免散”组、环磷酰胺组、环磷酰胺组 左旋咪唑组、环磷酰胺 “增免散”组,6日龄用鸡新城疫LaSota疫苗免疫点眼滴鼻,14、21、28、35日龄测定鸡血液ND抗体效价、免疫器官指数、细胞免疫功能、红细胞免疫粘附功能等,探索中药“增免散”的免疫增强机理。结果表明:中药“增免散”不但能显著提高Lasota活苗接种雏鸡的特异性HI抗体水平,延长其持续时间,能促进雏鸡免疫器官的生长发育,能显著增高雏鸡外周血T淋巴细胞ANAE阳性率,并对雏鸡红细胞免疫粘附功能具有显著促进作用,而且对环磷酰胺的免疫抑制有部分拮抗作用。 相似文献
82.
近期反刍动物营养与饲料研究述评 总被引:1,自引:0,他引:1
作者在比较近期国内外反刍动物营养与饲料研究侧重点的基础上,综述分析了热点研究项目的结果,并指出国内在进行同类研究时应注意的问题。本文对国内反刍动物营养学研究了及饲养实践均具有一定的参考价值。 相似文献
83.
mRNA差异显示技术是近年来国外发展起来的攻克隆新基因的一种新方法,同时已广泛用于人类基因调控和克隆研究。本文论述了这一新技术的原理、发展现状和在动物遗传育种中的应用领域。 相似文献
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Chang Liu Shike Shui Yangcheng Yao Cong Sui Hanwang Zhang 《Reproduction in domestic animals》2020,55(10):1418-1424
Phthalates, including di-(2-ethylhexyl)phthalate (DEHP), are common industrial chemicals in the environment. Recent evidence indicates that DEHP and its active metabolite mono-(2-ethylhexyl)phthalate (MEHP) negatively modulate reproductive functions and induce reactive oxygen species. Ascorbic acid (AA) is a dietary requirement for primates, and it acts as a potent free radical scavenger to protect tissues against oxidative stress. In this study, to investigate the toxic effects of MEHP on the follicle development and the beneficial role of AA, neonatal mouse ovaries were treated with different concentrations of MEHP with or without AA for 6 days. Then, the follicle constitution and oxidative status were compared in different groups. Results showed MEHP accelerated primordial follicle recruitment by increasing the percentage of primary and secondary follicles and decreasing the percentage of primordial follicles in the ovaries. Moreover, MEHP-induced ovarian oxidative stress by significantly increasing malondialdehyde (MDA) concentration and the expression of GSS and SOD1. When ovaries were co-administrated with MEHP and AA, follicle constitution was normalized, and the oxidative status was significantly decreased. These results suggested that AA ameliorated MEHP-induced ovarian oxidative stress and follicular dysregulation, which attested the clinical significance of AA for ovary protection in the case of MEHP exposure. 相似文献
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The objectives of this study were to examine the structural and metabolic integrity of isolated sheep external intercostal muscle bundles following variable lengths of preincubation (0 to 192 h). Samples of intact external intercostal muscle (10 to 15 g), with tendons attached, were prepared from growing wethers and maintained at their resting lengths during preincubation for 0 to 192 h. Protein synthesis (PS), protein degradation (PD), acetate oxidation and ultrastructural integrity of muscle samples were examined at 0 to 192 h, 0 to 96 h, 0 to 48 h and 0 to 96 h following isolation, respectively. Additionally, the effects of variable fetal calf serum (FCS) concentrations (0 to 20%; w/v) on PS and PD and acetate oxidation were examined. Rate of PS increased as preincubation time increased to 192 h; however, most of this increase was due to the proliferation of fibroblasts on the surface of the muscle sample. Addition of cytosine arabinoside to the incubation media prevented the fibroblast-dependent increase in PS; however, it did not entirely prevent the preincubation time-dependent increase in PS. Rate of PD increased greatly upon preincubation. The nitrogen balance of incubated muscles was negative at all times examined. Acetate oxidation was maintained through 12 h of preincubation and thereafter declined. Relatively normal myofibrillar structure was maintained through 48 h of preincubation; however, loss of mitochondrial integrity and dissolution of Z-disks at 48 h and at 96 h of preincubation were evident. Isolated tissues were able to respond to FCS concentration in medium following 48 h of preincubation. 相似文献
89.
通过对獒本杂交犬与当地土种犬发情时间、配种时间、产活仔数、幼犬初生重等的对比研究,发现獒本杂交犬发情时间集中在9~11月份,发情受胎率与母犬年龄及养殖条件有关,常规养殖下3~4岁獒本杂交母犬发情率为100%,2~4岁獒本杂交母犬受胎率达到100%。獒本杂交犬产仔力和哺乳性能等繁育性能指标明显高于本地土种犬,1~5岁龄杂交母犬的平均活产仔数显著高于当地土种犬(P(0.05);仔犬平均初生重显著高于当地土种犬(P(0.05);仔犬断奶平均个体体重显著高于当地土种犬(P(0.01);仔犬断奶平均窝重显著高于当地土种犬(P(0.05);幼犬断奶成活数比土种犬高14.3%。 相似文献
90.
饲料中水溶性肽含量测定 总被引:1,自引:0,他引:1
本标准规定了饲料中水溶性肽含量测定方法。本标准适用于饲料原料和配合饲料。检测范围为比色溶液中亮氨酸中氮含量4~20mg/L。 相似文献