开阳县田坝渔业小区实施大鲵养殖的技术措施

为摸索名优水产品养殖经验,加快我县水产养殖结构调整,促进我县渔业健康发展,2007年,我县申报实施了《贵阳市社会主义新农村基础设施建设——养殖基地基础设施建设——渔业小区建设》项目,在花梨乡翁昭村田坝组模拟大鲵生态生活习性新建了100口养殖池的大鲵小区,修建了养殖房、管理房、配套饵料鱼池、饵料鱼暂养池、引水管道等基础设施和配套设施,并于2008年3月25日起引种入场养殖。现将1年来小区开展大鲵人工养殖的技术措施报告如下。     

现浇楼板混凝土“二次法”施工工法

阐述了现浇楼板混凝土施工时,混凝土终凝前通过二次振捣的施工方法,愈合混凝土内部和表面初裂缝,以提高混凝土密实度,达到控制混凝土裂缝的目的。     

非洲菊切花保鲜剂的研究

本文研究由蔗糖、硝酸银、8-HQ(8-羟基喹啉)配制的不同保鲜剂对非洲菊切花插瓶寿命、水分平衡、鲜重变化及观赏值等指标的影响。结果表明：4％蔗糖 200mg／L硝酸银 200mg／L硫酸铝 320mg／L8-HQ的保鲜效果最好。     

牛烂蹄坏尾病致病弯角镰刀菌代谢产物分离鉴定

取金寨县耕牛烂蹄坏尾病致病弯角镰刀菌的玉米培养物，用丙酮提取，硅胶净化，制备型硅胶大板分离，得到无色针状结晶体，经质谱和红外光谱分析，并经相关谱图数据库联机检索、甄别，按其分子量、化学式及结构式确定为麦角甾醇，从而证实了致牛烂蹄坏尾病的弯角镰刀菌可产生麦角甾醇。     

Technical note: Development of an enzyme-linked immunosorbent assay for the determination of florfenicol and thiamphenicol in swine feed

One polyclonal antibody against florfenicol and thiamphenicol was produced and a competitive ELISA was developed for the detection of florfenicol and thiamphenicol in swine feed. The ELISA gave a 50% inhibiting concentration of 1.02 ng/mL for florfenicol. For swine feed fortified with 0.05 to 3.0 mg/kg, the interassay recoveries of florfenicol and thiamphenicol ranged from 86.4 to 118.6%, whereas intraassay recoveries of both drug ranged from 90.1 to 126.5% with less than 15% CV. Results obtained from HPLC-tandem mass spectrometry indicated this ELISA procedure could be used as a convenient method for rapid screening of florfenicol and thiamphenicol in swine feed.     

国际金融危机对粮食价格走势及上下游行业的影响

当前．国际金融危机的深化,正在对国内外粮食市场的供求格局和粮价走势产生深刻影响。而对国内市场粮价及粮食上下游企业的影响已经显现．今后将会日趋加重。今后几个月内国内粮价上涨的动力正在迅速弱化．下行的压力却在显著加大．但不排除半年乃至9个月后,国内外粮食价格出现较大反弹的可能性。     

巨型艾美耳球虫感染鸡空肠粘膜肥大细胞数量和组织胺含量的变化

本文借助于改良甲苯胺蓝染色法和荧光测定法，分别检测了巨型艾美耳球虫感染鸡空肠粘膜肥大细胞（ＭＭＣ）数量和组织胺（ＨＡ）含量的变化。结果表明，初次感染巨型艾美耳球虫之后，空肠ＭＭＣ呈减少趋势，至感染后第１４天回复至不感染对照组水平。而初次感染组相应空肠ＨＡ水平呈现出不显著波动后，在感染后第４天达最大值。在再次攻毒试验中，免疫攻毒组ＭＭＣ呈现水升趋势，尔后回复至不感染对照组水平；免疫攻毒组织相应肠道Ｈ     

貂,貉,犬病毒性肠炎快速诊断试剂的研究

用金黄葡萄球菌Ａ蛋白（ＳＰＡ）协同凝集方法（ＣｏＡ）对貂、貉、犬病毒性肠炎快速诊断的试剂进行了制备，确定了制备。艺及该制剂的标准操作程序。本制剂特异性强，不与健康动物的组织及其他几种病毒病（犬瘟热、犬传染性肝炎、狂犬病等）发生交叉凝集反应，灵敏度高，通过该制剂ＣｏＡ法与ＨＡ－ＨＩ法比较结果比现行的貂、貉、犬病毒性肠炎ＨＡ－ＨＩ诊断方法检出率高出３０％，同时具有微量、操作简便、易掌握、快速等特点，可在３～５ｍｉｎ获得诊断结果。     

我国丝绸面临的困境及成因浅析

丝绸业是以茧丝绸为纽带 ,以出口创汇为主的跨越农工贸的外向型行业 ,现在我国的蚕茧和生丝产量分别约占世界总产量的78%和 72 % ,丝类、绸缎和丝绸服饰的出口量分别约占世界贸易的 90 %、50 %和 45% ,1 950～ 1 998年间丝绸出口创汇达 42 2 .1 2亿美元 ,在世界丝绸业和国民经济中占有重要的地位。1　我国丝绸业面临的困境80年代后期 ,我国丝绸行业高速发展 ,产量大幅攀升 ,出口量迅猛增加 ,但至 90年代中期 ,全行业发生前所未有的亏损 ,近来 ,以加入ＷＴＯ为契机 ,行业情况有所好转 ,但也仍在困境中艰难跋涉 ,发展前景不容盲目乐观。自 1 9…     

DNA Barcoding:An Effective Tool for Species Identification of Lepidopteran Oak Pests

[Objective] The paper was to improve the efficiency and accuracy of early forecast of Lepidopteran oak-infesting pests.[Method] DNA barcoding technique was established for quick species identification using mitochondrial cytochrome C oxidase subunit Ⅰ(COⅠ) as the standard gene.This barcoding technique was used to amplify and sequence genomic DNA samples from eggs and pupae of 11 species of Lepidopteran pests collected from oak.[Result] The DNA barcoding standard genes of 594-708 bp were determined from eggs and pupae of Lepidopteran insects.There were differences of 0-2 bases in DNA barcode sequences between conspecific eggs and pupae,with the sequence identity of 99.7%-100%.The average content of A,T,G and C of DNA barcode sequences from Lepidopteran insects were 30.7%,38.5%,14.9% and 15.9%,respectively.The obtained DNA barcode sequences had 91.4%-100% identity and 0-8.6% difference degree with GenBank-deposited DNA barcode sequences from organisms of the genetically-closest relationship.Among them,DNA barcode sequences from egg and pupa samples of 10 Lepidopteran insects(No.1-20) had 99%-100% identity and 0-1.0% difference degree with homologous sequences in GenBank database,while the remaining samples(No.21-22) had high difference degree(8.6%) with homologous sequences.[Conclusion] The established DNA barcoding technique is an effeetive tool for species identification of Lepidopteran pests using genomic DNA from eggs and pupae of Lepidopteran insects.