内蒙古牧区传统乳制品中耐酸及耐胆盐植物乳杆菌的筛选

试验对从内蒙古通辽市科左后旗和扎鲁特旗牧民家的乳样中分离的27株植物乳杆菌的耐酸及耐胆盐特性进行研究。结果显示，有20株菌能耐受0．3％的牛胆酸钠，且对pH3．0的生长环境表现出不同程度的耐受性。其中8株菌耐受性较强，存活率最高可达150．6％，其余菌株从15．5％-39．8％不等。     

脱脂乳发酵体系中乳酸菌的高效离子交换色谱分析

应用高效离子交换色谱对脱脂乳发酵体系中的嗜热链球菌和保加利亚乳杆菌进行色谱表征。结果表明，单菌培养过程中保加利亚乳杆菌菌体形态的变化在其相应色谱行为上得到了体现，二者的色谱峰有比较好的稳定性。两种菌的峰面积与菌数之间呈现出良好的线性关系，可决系数R^2分别为0．9957和0．9916。因此本方法的建立，可用于酸奶发酵过程中乳酸菌的分析与表征，有望为乳酸菌混合物的研究提供一种快捷的新方法。     

不同碳水化合物对犊牛消化生理功能的研究进展

碳水化合物是多羟基醛、酮及其衍生物的总称,是反刍动物瘤胃微生物的主要能量来源,在犊牛营养中起着重要的作用.介绍了碳水化合物的分类及在瘤胃中的消化代谢情况,并就近年来不同类型碳水化合物在犊牛开食料中的应用进行综述.     

怎样选择一头适合防卫训练的小犬

训练是爱好者必须面对的问题,同一品种内,血统将是知道小犬潜能的关键。这一点在众多防卫犬的训练、应用和成绩中已经证明,并且这些显著的特性同样遗传在它们的子女和全部的后代中。这些特性,不仅包括犬的身体结构,更重要的是它们与生俱来的气质和性格。     

PAVC乳液防治苹果树腐烂病的初步研究

通过温室和大田进行PAVC乳液防治苹果树腐烂病的试验,结果表明:PAVC乳液能很好的促进伤口的愈合,效果优于传统的石蜡、羊毛脂、食用油和泥,比对照提高了71.13%;对苹果腐烂病的田间相对防效为88.17%,次年复发率为4.2%。     

甘肃省靖远小口枣生产情况调查

靖远小口枣原产于甘肃省靖远县石门乡小口村,以果大肉厚核小,甜脆清香而闻名遐迩。其果肉绿白色,质疏松,汁少,味甜,为优良的鲜食和制干兼用品种。曾于1988年在甘肃省枣鉴评会上获省优质产品称号;1991年在石家庄全国林果产品博览会获金奖;1996年在甘肃省名优特林果鉴评会上获银奖;2003年在中国首届沙产业博览会上获优质产品奖。20世纪90年代前,靖远小口枣仅分布在小口村附近的几个村,栽植面积较小,且零星栽植的植株占有一定的比例,生产规模小,不适应商品化生产的需要。为了发挥地方名优特产的资源优势,近20年来,靖远县将小口枣作为林果产业的…     

一起动物检疫行政不作为案的诉讼启示

1案例2004年12月10日,我市某区人民法院公开审理了某生猪屠宰企业诉动物检疫站不履行法定检疫职     

灵芝子实体及其深层培养产物特性的研究

本文对灵芝（Ｇ—６）子实体与发酵菌丝体的粗多糖、多糖组份、粗蛋白及氨基酸组成进行了比较。结果表明：发酵菌丝体中含有子实体中所具备的组份,但各组份在量上不同。菌丝体中的粗多糖及多糖含量均高于子实体分别为子实体的２．２６倍、３．５倍。菌丝体的蛋白含量明显高于子实体,比子实体高２．４７倍,且两者氨基酸组成不一致。其中子实体中必需氨基酸为总氨基酸量的５８．４％,而菌丝体中必需氨基酸为总氨基酸的４５．２％。     

变叶海棠变异类型的初步研究

应用形象化散点图研究变叶海棠的起源和进化。结果表明,其变异类型可能系变叶海棠和陇东海棠或花叶海棠之间的天然杂交种,该假设需要用现代新方法来证实。     

Molecular Identification and Phylogenetic Grouping of Diaporthe phaseolorum and Phomopsis longicolla Isolates from Soybean

ABSTRACT Diaporthe phaseolorum and Phomopsis longicolla isolates from soybean were examined using traditional mycological characteristics and molecular methods. Cultural characteristics including types of fruiting bodies and conidia were assessed for isolates collected from soybean stems and seeds. Cultures were identified as P. longicolla, D. phaseolorum var. caulivora, D. phaseolorum var. meridionalis, or D. phaseolorum var. sojae. Molecular markers for these groups were developed and analyzed using polymerase chain reaction restriction fragment length polymorphisms (PCR-RFLP) and DNA sequencing in the internal transcribed spacer (ITS) and the 5.8S ribosomal DNA. The ITS(4) and ITS(5) primers amplified PCR products for all isolates studied. Gel electrophoresis of undigested PCR products and DNA sequencing produced various fragment lengths including 604 bp for P. longicolla, 602 and 603 bp for D. phaseolorum var. caulivora, 603 bp for D. phaseolorum var. meridionalis, and from 597 to 609 bp for D. phaseolorum var. sojae. Digestion of these PCR products with enzymes AluI, HhaI, MseI, RsaI, and ScrFI resulted in distinct bands for identification of P. longicolla and the varieties of D. phaseolorum I. All P. longicolla, D. phaseolorum var. caulivora, and D. phaseolorum var. meridionalis isolates were distinguished using AluI and HhaI with RsaI or ScrFI. The banding patterns of D. phaseolorum var. sojae isolates were complex and were separated into 11 subgroups after digestion with AluI, HhaI, MseI, RsaI, and ScrFI. Phylogenetic analysis of 20 isolates of D. phaseolorum and P. longicolla based on the DNA sequence of the ITS region resolved six clades termed A, B, C, D, E, and F. Clade A included all sequenced D. phaseolorum var. caulivora isolates, two from Italy and one from the United States. Isolates in clade B were exclusively associated with D. phaseolorum var. meridionalis. Clades A and B formed a well-supported monophyletic group. Isolates in clades C, D, E, and F were morphologically defined as isolates of P. longicolla, D. phaseolorum var. sojae, and Diaporthe spp. The ITS sequences similarity of seven geographically diverse P. longi-colla isolates illustrated that P. longicolla isolates have a similar genetic background, with some affiliations to some D. phaseolorum var. sojae isolates. Morphological characteristics of the isolates along with the terminal clades of the ITS phylogeny suggest that P. longicolla is an individual species, D. phaseolorum var. caulivora and D. phaseolorum var. meridionalis are varieties of D. phaseolorum, and D. phaseolorum var. sojae is either several varieties of D. phaseolorum or possibly several distinct species.