Isolation and characterization of microsatellites in Bambusa arundinacea and cross species amplification in other bamboos

Isolation and characterization of microsatellites was analysed in Bambusa arundinacea and cross species amplification studied in other bamboos. Microsatellites, tandem repeats of short nucleotide (1–6 bp) sequences, are the DNA marker of choice because of their highly polymorphic, ubiquitous distribution within the genome, ease of genotyping through Polymerase chain reaction, selectively neutral, co‐dominant and multiallelic nature. Six microsatellites, three polymorphic and three monomorphic have been characterized for the first time in a bamboo species, Bambusa arundinacea belonging to the family Poaceae. The numbers of alleles per locus ranged from 2 to 13. Cross species amplification was tested in 18 other bamboo species. Monomorphic simple sequence repeats (SSRs) were found to be cross amplified in most of the species tested and polymorphic ones in only three to four species. The utility of SSR loci in a genetic diversity study of B. arundinacea and other cross‐amplified bamboo species has been discussed. This study will help in population genetic studies in bamboo species.     

Exploration of the Sustainability Design for Industrial Building

Within the domain of comprehensive architecture, the general instructive principles for the sustainability design are applicable to that of industrial building. Based on the analysis of excellent precedents, in this paper, some useful experiences are summarized. It is pointed out that the sustainability design is feasible and should be compulsive for industrial building.     

Determination and Evaluation of Saccharides Content in Extract of Herba Spirodelae Seu Lemnae Polysaccharides

In the course of searching immunodulators from natural herbal sources,the polysaccharide has been isolated from the water extract of duckweed(Herba Spirodelae seu Lemnae).The authors aim at investigating the methods that determine the content of total saccharides in the mixture,accordingly,to be used as a quality control in the procedure of producing polysaccharides from duckweed.The contents of saccharides in the extraction were mensurated by spertrophotometry of sulphuric acid-phenol and sulphuric acid-anthrone.And the validitiy of methods was evaluated through some indexes such as linearity and range,precision,stability,and accuracy.The results reveal the methods are convenient,rapid,accurate,and high sensitive,thereby leading to a wide and effective use in determining the saccharides contents in polysaccharides extraction from Herba Spirodelae seu Lemnae.     

Nonlinear Characteristic Modeling of Servo Value on Hydraulic Bending Roll System''s Dynamic Characteristic

Nonlinear characteristic of servo value is the important factor influencing hydraulic system, the model of electro-hydraulic servo pressure control system is designed. In this model, flux-equation of servo valve is specially applied and some non-linear characteristics are considered. Furthermore, SIMULINK toolbox of MATLAB language software is applied to solve mathematic model and simulation curves of some variables are obtained. The calculation results are in good agreement with the experimental results.     

Numerical Prediction of Frequency of Delaminated Advanced Grid Stiffened plate

The paper aims to investigate natural frequency of delaminated advanced grid stiffened composite plates by hump resonance method.The composite laminated plate element and the rib element are adapted to simulate the laminated plate and the ribs.Based on the composite laminated plate and beam element model considering first-order shear effect,a damping model on the basis of Rayleigh damping model in conjunction with Adams' strain energy method(MSE) and a Hertz nonlinear dynamic contact model to avoid the overlap and penetration phenomenon between the upper and lower sub-laminates at the delaminated region.A numerical analysis method of dynamic response for the delaminated composite plates is carried out by precise time-integration method,and then the natural frequencies are achieved when the dynamic deflections reach the hump peak.For the frequency-domain analysis provided in the former research papers,it is a difficult problem to avoid the embedded phenomenon in the mode shapes at the delamination zone,while in the current time-domain analysis,the embedded phenomenon can be successfully overcome by using nonlinear contact model.By vibration excitation test,the specimens were excited by different excited frequency and the amplitude frequency response characteristics obtained are used to extract the frequency.The extracted frequencies of the narrow delaminated composite plates measured,which agrees well with the predicted natural frequencies.     

Dynamics of Actin Polymerization

The process of actin polymerization and depolymerization have some relation with its function,the activation of such process is compatible to its functions. The polymerization progress is divided into four steps: The first step is the activation of the monomer actin. The nucleation of actin nucleus forms in the second step which limits the polymerization speed. The elongation of the F-actin filaments happens in the following step. In the last step, a steady-state is attained, the length of the filament does not change because the polymerizing rate in the barbed end is the same with the depolymerizing rate in the pointed end. Some factors which affect the polymerizing process, such as nucleotide, actin related proteins are discussed. The aim is to know more about how to assemble the complicated system with biomacromolecule and what to function.     

A Study on the Model of Surface Storm-Water Runoff at Urban Gully

On the assumption that the runoff on the catchment of each gully flows into the gully itself, in this paper, a model of surface storm-water runoff at urban gully is presented, which includes runoff sub-model and concentration sub-model.The runoff sub-model describes the rainfall event with instantaneous rainfall intensity, the runoff-generated event with runoff coefficient of integrative area and the runoff converged event with the principle of equal time of flowage.The concentration sub-model shows that the pollutant concentration event only changes with the runoff event at the gully.In this paper, the concrete form of the model for two gully inlets in Shapingba District of Chongqing is given.The model is calibrated with the data from the synchronized measurements of water quantity and quality at the two gully inlets obtained from July to September 2000.The relative error is calculated by comparing the model predictions with the field-measured results.     

Biodegradation of Linear Alkyl Benzene Sulphonate

The paper researches on the degradation of LAS (Linear Alkyl benzene Sulphonate) by combining AS1.860 immobilized with low intensity ultrasonic irradiation. The paper also discusses the influence of pH, rotary velocity, LAS concentration and the condition of low intensity ultrasonic irradiation on the degradation of LAS, the degradation rate of LAS is the main index of our experiment, the results of orthogonal test shows that low ultrasonic irradiation can increase the metabolizing of microorganism cells and facilitate the biodegradation of immobilized cells to LAS, here we research the degradation condition of 50mg/L LAS simulation wastewater with low ultrasonic irradiation, and the results show that the influence is obvious, the optimization degradation rate is about 83%, nine point five percent higher than that of the immobilized cells without ultrasonic irradiation.