黄檗无性系种子园母树生长性状评价

对黄檗种子园4个种源的26个无性系的树高当年生长量、树高和冠幅进行了分析。结果表明,黄檗不同种源间树高当年生长量、树高和冠幅差异均达到极显著水平(F值分别为7.84**、19.28**和11.62**),各性状遗传力分别为0.25、0.45和0.33,受中等遗传控制。各区无性系间树高当年生长量、树高和冠幅差异均显著或极显著。通过多指标综合评分及最优性状综合分析,选出23号、1号、2号、4号、24号、10号和25号为优良无性系,其中1号为最优无性系。     

永久性贮水池在水灭火法扑救森林火灾中的运用

本文简要阐述了水灭火法灭火原理与系统组成,提出通过建立永久性贮水池促进水灭火法将成为我国林火扑救重要手段的发展设想。     

陕西富硒与非富硒地区山羊组织硒含量和GPX-7基因表达的比较

为了探讨陕西紫阳县(富硒地区)山羊各组织中微量元素硒的含量和谷胱甘肽过氧化酶7(Glutathione Peroxidase 7,GPX-7)基因的表达情况,以及组织中的硒沉积量对GPX-7基因表达的影响。利用原子荧光光谱法对年龄相仿、体质量相近的紫阳县双安镇(n=7)和广成镇(n=7)和宝鸡市陈仓区白山羊体内硒含量进行了测定;同时运用实时荧光定量PCR(qRT-PCR)相对定量技术测定GPX-7基因mRNA的相对表达量。紫阳双安镇山羊硒含量显著高于紫阳广成镇(P0.05),同时紫阳县均显著高于宝鸡陈仓区(P0.05);但是紫阳双安镇与紫阳广成镇山羊肺脏中硒含量差异不显著(P0.05)。GPX-7基因在各组织器官中广泛表达,但是不同地区的山羊表现出不同的组织差异性。紫阳双安镇山羊心脏、脾脏、肾周脂肪和背最长肌组织中mRNA水平与紫阳广成镇差异不显著(P0.05),但是紫阳地区均显著高于宝鸡陈仓区(P0.05)。在肺脏、肾脏、股二头肌和淋巴组织中,来自不同地区的山羊GPX-7基因mRNA的表达量地差异均两两显著(P0.05),其中紫阳广成镇山羊肺脏的表达量显著高于其他两个地区(P0.05)。由此可见,山羊各组织器官中微量元素硒的沉积量和GPX-7基因mRNA的表达量表现出较为一致的地域趋势,即紫阳双安镇紫阳广成镇宝鸡陈仓区,这表明微量元素硒上调GPX-7基因的表达,但是具有组织差异性。     

蓟马采集和玻片标本的制作

\t\t\t\t\t目的\t\t\t\t\t丰富中国呆蓟马属团蓟马种类,为呆蓟马属团的鉴定提供参考信息。\t\t\t\t\t\t\t\t\t\t\t\t\t方法\t\t\t\t\t标本采集采用拍盘法,标本保存在75%的酒精中,带回室内进行玻片标本制作,在生物显微镜下进行鉴定。\t\t\t\t\t\t\t\t\t\t\t\t\t结果\t\t\t\t\t记述了蓟马亚科呆蓟马属团一中国新纪录属塔姆蓟马属(Tameothrips)及1新纪录种芦竹塔姆蓟马(T. arundo),详细描述了该种的形态鉴定特征,编制了中国已知呆蓟马属团属种名录及分属检索表。玻片标本保存于云南农业大学植物保护学院昆虫系。\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t结论\t\t\t\t\t塔姆蓟马属(Tameothrips)为中国新纪录属,中国目前已记录呆蓟马属团9属21种。\t\t\t\t\t\t\t\t\t     

Association of tumor budding with tumor-infiltrating lymphocytes and prognosis in breast cancer patients

AIM: To investigate the relationship of tumor budding with clinicopathologic parameters, tumor-infiltrating lymphocytes (TILs) of tumor microenvironment and the prognosis in breast cancer patients.METHODS: A total of 178 HE section samples were collected from the breast cancer patients treated with surgery in the First Affilated Hospital of Jinan University during Jan. 2012 to Dec. 2016. The tumor budding and stromal tumor-infiltrating lymphocytes were observed under light microscope. The correlation of tumor budding with the clinicopathologic status and TILs were analyzed by χ² test. Kaplan-Meier survival analysis and Log-rank test were used to estimate the disease-free survival (DFS) and overall survival (OS).RESULTS: High tumor budding level was associated with more positive lymph nodes, higher grade, and more lymphovascular invasion. In addition, the patients with higher tumor budding level showed fewer TILs, while the patients with lower tumor budding level had more TILs. Furthermore, the patients with higher tumor budding level had a worse disease-free survival and overall survival than those with lower tumor budding level.CONCLUSION: Tumor budding is significantly associated with adverse clinicopathological characteristics of breast cancer and negatively correlated with TILs. Therefore, tumor budding may serve as a potential biomarker to predict the prognosis of breast cancer.     

Effects of puerarin on apelin-12, angiotensin II,NO and blood pressure in renovascular hypertensive rat

AIM: To investigate the effects of puerarin on blood pressure in renovascular hypertensive rats, and to measure puerarin-induced changes of apelin-12, angiotensin II (Ang II) and nitric oxide(NO), the factors related to development of hypertension. METHODS: Sixty-five male Sprague-Dawley rats were used, of which 8 rats were randomly selected as sham operation group, and the remaining were used to make two-kidney, one-clip model. The rats that met the criterion for Goldblatt hypertensive rat model were randomly allocated into 5 groups: high-, middle- and low-dose puerarin groups, captopril group, and model group. The drugs were administered for 6 weeks. Blood pressure was measured every 2 weeks. Six weeks after treatment, all rats were sacrificed under deep anesthesia. Blood and kidney samples were collected. The level of apelin-12 in serum and kidneys was detected by ELISA. The level of Ang II in plasma and kidneys was measured by radioimmunoassay. NO level in serum was examined by nitrate reductase assay. RESULTS: Puerarin had an antihypertensive effect in a dose-dependent manner. Marked decreases in the level of serum apelin-12 in high- and middle-dose puerarin groups were observed(P<0.01). Puerarin at low dose did not cause obvious change in the content of apelin but still reached significant level (P<0.05). As the dose of puerarin went up, the level of apelin-12 in the kidneys was gradually decreased. Puerarin at high and middle doses obviously reduced the level of AngII in plasma, while purarin at low dose did not produce any significant effects. Puerarin at high and middle doses markedly increased the level of NO in serum, but puerarin at low dose did not induce any significant changes. CONCLUSION: Puerarin has an antihypertensive effect, and its mechanism may be related to inducing the changes of apelin, Ang II and NO, and regulating the balance among those factors.     

Statistical Model on the Time and Time Difference Error of Arrival in TDOA Location Estimation

Additional time delay of arrival resulted from non-line of sight(NLOS)propagation and muti-path effect in(cellular) environment is the dominant factor resulting in TDOA location error,study on the statistical property of TDOA error is useful for improving the accuracy of TDOA location.Based on the exponential distribution property of NLOS propagation delay,and considering the system error resulted from the signal detection,the authors establish a statistical model of arrival time and a model of TDOA error distribution by statistics when non-light of sight and multi-path propagation exist.The models reflect the statistical property of arrival time and TDOA error in cell network,the simulation shows that the proposed models have a good accuracy and a satisfactory behavior.     

发菜生存与生长分布的微生态环境

通过野外调查 ,研究和分析了对发菜生存与生长发育起直接作用的微土壤、微气候、微地形、微植被和风向等微生态环境。发菜对生存环境的选择性很强。其中 ,干一湿交替的微气候环境是一个起决定作用的关键性因子 ,是发菜生存和生长发育的必要条件。一方面 ,发菜藻体在干燥的状态下 ,有利于干物质的积累 ,是发菜生存和生长的必要前提 ;另一方面 ,发菜藻体只有在一定湿润状态下 ,才能生长发育。由于干旱气候环境所限 ,野生发菜的生长速度极为缓慢。