Inhibitory effect of apolipoprotein A-I mimetic peptide D-4F on scavenger receptor A1 in macrophage-derived foam cells

AIM:To investigate the inhibitory effect of apolipoprotein A-I mimetic peptide D-4F on the scavenger receptor A1 (SR-A1) in macrophage-derived foam cells induced by oxidized low-density lipoprotein (ox-LDL). METHODS:RAW264.7 cells were pretreated with different concentrations (12.5, 25 and 50 mg/L) of D-4F or 50 mg/L inactive control peptide scrambled D-4F (sD-4F) for 1 h or endoplasmic reticulum stress (ERS) inhibitor 4-phenylbutyric acid (5 mmol/L) for 30 min, followed by the treatment with 100 mg/L ox-LDL for 12 h. In addition, the cells were pretreated with 50 mg/L D-4F or sD-4F for 1 h, and then stimulated with 2 mg/L tunicamycin (TM; an ERS inducer), for 4 h. The viability of the cells was measured by MTT assay, and the content of intracellular total cholesterol (TC) was measured by a tissue/cell TC assay. The protein and mRNA levels of SR-A1 and glucose-regulated protein 78 (GRP78) were analyzed by Western blotting and quantitative real-time PCR, respectively. The fluorescence intensity of DiI-ox-LDL in the cells was detected by a multifunctional microplate reader. RESULTS:D-4F significantly reduced ox-LDL-induced macrophage injury and intracellular cholesterol accumulation, and attenuated the ox-LDL-induced expression of SRA1 and GRP78 in a dose-dependent manner. Additionally, D-4F significantly inhibited the TM-induced protein expression of SR-A1 and GRP78, and attenuated the uptake of ox-LDL by macrophages. CONCLUSION: D-4F reduces ox-LDL-induced macrophage cholesterol accumulation and injury by inhibiting SR-A1 expression. The mechanism may be related to the inhibition of ERS signaling pathway mediated by GRP78.     

平欧杂种榛果实发育过程中Vc含量的变化研究

以7年生平欧杂种榛‘82—11’、‘84—237’及‘84-402’为研究对象,在果实成熟期每隔3。6天,采用2,6-二氯靛酚滴定法测定Vc含量,分析其果实发育过程中Vc含量变化。结果表明：幼果迅速发育期,Vc相对含量和绝对含量大幅度下降,平均降幅为90．67％和64．4J4％;从果壳硬化期到果实成熟,Vc相对含量和绝对含量变化不大,降幅较小,其平均下降幅度为39．72％和30．47％。     

5-甲基多菌灵的防霉性和抗流失性

为开发防霉效果优良的木材用防霉剂,对2-氨基甲酸甲酯苯并咪唑(多菌灵,MBC)进行改性,得到2-氨基甲酸甲酯-5-甲基苯并咪唑(MMBC).经检测MMBC处理材的防霉性和抗流失性,结果表明:MMBC的防霉性优于传统防霉剂五氯酚钠,且流失率较改性前有所降低.     

秸秆深施还田历经时间对蓄水效果的影响

在实验室人工控制降水条件下,对黑龙江省黑土耕地土壤深施3种不同作物秸秆,研究其蓄水效果及土壤含水量随历经时间的变化规律.结果表明,秸秆深施初期在各深度土层上大豆和玉米秸秆的蓄水效果显著(P＜0.05).秸秆深施9个月在距离秸秆深施较近15和20 cm土层处玉米、水稻和大豆秸秆有显著效果.秸秆深施22个月仅玉米秸秆在20 cm土层处有效果.随着深施秸秆历经时间的延长各种秸秆蓄水效果整体呈下降趋势.在水平方向上,随着距秸秆深施处距离的增加,土壤含水量逐渐减小.     

梭梭树龄与肉苁蓉种子产量关系的研究

以梭梭和肉苁蓉为试验材料,研究了梭梭树龄与肉苁蓉种子产量的关系.结果表明:1)梭梭基径粗、株高、冠幅、肉苁蓉直径、花序长、蒴果数、有效果数、单蒴果重和单株种子产量与梭梭树龄呈显著正相关,相关系数在0.93以上.2)随着梭梭树龄增长,与对照相比,单株肉苁蓉种子产量提高了2～5倍.直径＜0.5 mm的种子所占比例下降,直径为0.5～0.7 mm种子所占比例提高.3)5年生及以上的梭梭所寄生的肉苁蓉直径和花序长可达21.72 mm和30.8 cm以上,并且蒴果数、有效果数和单株种子产量分别可达143个、128个和11.810 g以上.5年生及以上的梭梭可作为寄主培育肉苁蓉留种植株.     

平阳县气象局新旧站址气象资料对比分析

通过对温州平阳气象站新、旧站址2012年同期气温、降水量、相对湿度、风向风速等观测资料的对比,发现新站气温低于旧站;新站降水量多于旧站;新站年平均相对湿度偏大旧站;风速新站大于旧站。新站和旧站降水量、相对湿度一致性最差,表现出明显的局地性特点;风速一致性较好,观测数据较稳定。显著性检验结果表明,平均气温、降水量和平均相对湿度差异性不显著,表明新站此3个要素资料可与旧站资料续接合并使用,而平均风速因为新站迁移至东门山顶,显著性差异大,与旧站资料不能续接使用。     

晋鲜糯玉米系列品种的选育与推广应用

介绍了晋鲜糯玉米系列品种晋单(糯)41号、鲜糯2号、晋鲜糯6号、晋糯8号、鲜甜2号、晋糯5号、晋糯10号这7个品种的选育过程、选育方法、品种特征特性以及各品种推广模式、品种应用情况,可为糯玉米育种和种植加工者提供参考依据。     

烟草根结线虫病研究概况及其防治措施

烟草根结线虫病可对各大产烟区造成严重经济危害,已成为近年来烟草病虫害防治的重点。为有效且有针对性地开展烟草根结线虫病的防治工作,通过阐述烟草根结线虫病的病原物、线虫-土壤-作物之间联系及其综合防治的途径,对其新型生物防治的前景进行了展望,以期对烟草根结线虫病的综合防治研究提供理论参考。     

洱海北部地区大蒜‖蚕豆模式作物生长发育规律及经济效益分析

本文从经济可行性和生态适应性角度分析了大蒜‖蚕豆模式在当地种植的适宜性。在洱海北部地区罗时江边上关镇兆邑村开展田间小区试验,经田间测定和样品经室内分析化验后,依据所得数据分析干物质累积规律、根瘤菌变化、根系分布、作物群体生长率土地当量比等状况。结果表明,间作能增加后期干物质累积量,能提高大蒜叶绿素含量,提高后期光合利用效率。间作模式可以促使大蒜和蚕豆的根系向下生长。间作比单作效率高,间作的增产率为15%。本研究为增加当地农田生物多样性,合理安排当地种植结构提供参考。     

Development of SRAP Markers Linked to a Gene for Stem Nematode Resistance in Sweetpotato, Ipomoea batatas (L.) Lam.

Sequence-related amplification polymorphism (SRAP) markers closely linked to stem nematode resistance gene were developed in sweetpotato, Ipomoea batatas (L.) Lam. Using bulked segregant analysis (BSA), 200 SRAP primer combinations were screened with the resistant and susceptible bulked DNA from the 196 progenies of an F1 single-cross population of resistant parent Xu 781×susceptible parent Xushu 18, 77 of them showed polymorphic bands between resistant and susceptible DNA. Primer combinations detecting polymorphism between the two bulks were used to screen both parents and 10 individuals from each of the bulks. The results showed that primer combination A9B4 produced 3 specific bands in the resistant plants but not in the susceptible plants, suggesting that the markers, named Nsp1, Nsp2 and Nsp3, respectively, linked to a gene for stem nematode resistance. Primer combination A3B6 also produced a SRAP marker named Nsp4 linking to the resistance gene. Amplified analysis of the 196 F1 individuals indicated that the genetic distance between these markers and the resistance gene was 4.7, 4.7, 6.3, and 9.6 cM, respectively.