Effect of Vitamin E on Bovine Granulosa Cells Apoptosis and Proliferation through Cx43

The aim of this study was to determine the effect of vitamin E on Cx43,the mechanism and function of vitamin E on bovine granulosa cells apoptosis and proliferation.In this study,granulosa cells were isolated from bovine ovary and cultivated in vitro by adding different concentration of vitamin E (0,25,50,100,200 and 500 μmol/L) for 24 h.After cultured,apoptotic cells were detected by FCM,mRNA expression levels of BCL2/BAX、P53 and Cx43 genes were determined by Real-time PCR and cell proliferation was detected by CCK8.The results showed that compared to control group,100 μmol/L vitamin E could significantly inhibit the apoptosis of granulosa cells (P<0.05).Real-time PCR detection results showed that vitamin E significantly changed the mRNA expression levels of BCL2/BAX,P53,Cx43 genes (P<0.05).Vitamin E could significantly improve granulosa cells proliferation when granulosa cells were treated for 24 and 36 h (P<0.05).The results provided a theoretical basis on further analysis for studing the influence mechanism of vitamin E on oocytes development and maturity,and improvement of female animal reproduction by influencing granulosa cells proliferation and apoptosis.     

影响‘小凤兰’根状茎增殖、分化和壮苗的因素研究

以‘小凤兰’根状茎为试材,研究了影响‘小凤兰’根状茎增殖、分化、生根壮苗的因素。结果表明:MS基本培养基、蔗糖30g/L、6-BA 2.0mg/L+NAA 0.5mg/L、活性炭0.5g/L有利于根状茎增殖,而添加20%椰汁和番茄汁40g/L会抑制根状茎增殖。1/2MS基本培养基、蔗糖30g/L、6-BA 2.0mg/L+NAA 0.2mg/L、20%椰汁、1 000lx光照强度有利于根状茎分化,不加活性炭有利于芽分化,但抑制根分化,而添加活性炭则抑制芽分化,促进苗分化。添加土豆泥60g/L显著促进试管苗生长,3~4cm高的苗在培养基1/2MS+6-BA 0.1mg/L+NAA0.5mg/L+蔗糖20g/L+土豆泥60g/L+卡拉粉8g/L+活性炭0.5g/L中培养40d即可移栽。     

三种地被菊种子萌发及幼苗生长的化感效应比较

以天人菊、金鸡菊和蛇鞭菊为试材,比较两两混种与单种在种子萌发和幼苗生长阶段相互间的化感效应强度。结果表明:两两混种下,金鸡菊、蛇鞭菊对天人菊生长起促进作用,化感效应强度为金鸡菊蛇鞭菊;天人菊、蛇鞭菊对金鸡菊生长起抑制作用,化感效应强度为天人菊蛇鞭菊;金鸡菊、天人菊对蛇鞭菊生长起抑制作用,化感效应强度为金鸡菊天人菊。     

抗青枯病樱桃番茄品种的初步筛选及评价

对28个樱桃番茄品种的田间抗病性、植株类型、单果质量、果色、可溶性固形物含量等主要经济性状进行调查。试验结果表明,感病品种红艳发病最重,发病率为73.67%,其次是红箭、红日和金艳,发病率分别为23.33%、21.67%和20.33%,其他品种发病率都在20%以下;28个品种的果实颜色、大小、口感等差异明显,除黑宝石映泰及黄金蛋可溶性固形物含量在4.10%以下外,其他品种可溶性固形物含量都达5.20%以上。综合分析,筛选出绿天使、粉仙、红娘9号、绿圆、格丽贝、红椭圆等11个抗青枯病且经济性状优良的樱桃番茄品种,为优质抗青枯病樱桃番茄的选育和推广提供了参考。     

社会主义新农村建设目标及实现

“生产发展、生活宽裕、乡风文明、村容整洁、管理民主”是社会主义新农村建设的主要目标,它是物质文明、精神文明和政治文明的具体体现。这些目标的实现,有赖于党的坚强领导,农民生活条件的改善和自身素质的提高。     

华南三省(自治区)部分西番莲种植区花叶病病原检测及EAPV多聚蛋白基因序列分析

<正>西番莲(Passiflora edulis Sims.)是热带亚热带地区重要经济作物,我国福建、广东、广西、海南等地广泛种植[1]。调查发现,我国南方多地西番莲花叶病为害严重,症状表现为叶片花叶、黄化、斑驳、畸形,甚至整株褪绿,严重影响西番莲的产量和品质。已报道能引起西番莲花叶症状的病毒有：黄瓜花叶病毒属(Cucumovirus)的黄瓜花叶病毒(cucumber mosaic virus, CMV),     

丛枝菌根真菌接种对白车轴草耐盐性的影响

丛枝菌根真菌(AMF)可与植物共生形成复杂的菌丝网络,影响植物生长及抗逆能力。目前关于AMF对白车轴草耐盐性的影响尚存争议,本研究采用盆栽试验,研究在盐胁迫条件下(NaCl浓度为150 mmol·L^-1),接种AMF对拉丁诺白车轴草耐盐性的影响。结果表明,在盐胁迫条件下,与对照相比,白车轴草的生长与生理指标均受到抑制。盐胁迫下接种AMF后,白车轴草株高、干重、PSⅡ最大光能转换效率和相对含水量均有增加,丙二醛(MDA)含量以及相对电导率有所降低,渗透调节物质均有提高,其中可溶性糖(SS)和游离脯氨酸(Pro)含量分别提高了32.03%和9.42%。说明盐胁迫抑制白车轴草的生长,接种AMF增强了白车轴草抗逆适应能力,促进白车轴草的生长,增加渗透调节物质含量,提高白车轴草耐盐胁迫的能力。     

利用SSR标记构建贵州山区玉米杂交种的指纹库

对贵州山区目前在生产上正在使用的西山系列玉米杂交种12份进行了SSR分析。从60对引物中筛选到稳定性好、多态性丰富的8对引物作为核心引物。用这8对引物的指纹组合构建了贵州山区西山系列玉米杂交种的DNA指纹数据库。结果表明,利用SSR技术进行玉米杂交种真实性的鉴定是可行、有效的。     

Application of Slicing in Component-based System Regression Testing

In component-based system regression testing, the dependence model of component-based system should be built in order to confirm the part which will be affected by modify. Since high reusability and high complexity, the existing dependence models may not be applicable to component-based systems, a hierarchical dependence model is proposed to describe component-based. Through the forward slicing of modified points in the model, the modified and affected parts can be gotten, then traverse these parts and get affected testing path, thereby regression testing examples can be chosen effectively, the efficiency of regression testing can be improred.     

Advances in Pharmacological Studies of Coptis Chinesis

A Coptis Chinesis is the dry rhizoma of ranunculaceous Coptis Chinesis Franch or Coptis deltoidea C. 7. Cheng et Hisao, or Coptis teeta Wall. It is the traditional Chinese herb, which is used in the contagious diseases. In recent years, its new pharmacological actions have been reported, which are antitumour, hypoglycemic response, immunoloregulation, improving the functions of cardiovascular. The important pharmacological functions of Coptis Chinesis are hypoglycemic response, improving the functions of cardiovascular, antitumour. With the deeply researching of Coptis Chinesis pharmacology, and the continuous founding of pharmacological mechanism, new preparation will be widely applied in the clinic.