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91.
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93.
构建稻瘟病菌有性后代随机群体是研究目的基因遗传特点,乃至克隆目的基因的重要基础,但是在建立有性后代随机群体时,可能会有无性后代污染。采用先分离单个子囊,待其长成菌落并产生分生孢子后,再分离单个萌发的分生孢子的方法构建稻瘟病菌有性后代随机群体。利用基于重复DNA序列Pot2的rep-PCR方法对分离得到的有性后代进行DNA指纹分析,并评价构建的稻瘟病菌有性后代群体的质量。结果表明,构建的有性后代随机群体共包含176个后代。每个后代个体的DNA指纹图谱均与两亲本的DNA指纹图谱不一致;有性后代之间的DNA指纹图谱也彼此不一样,说明得到的有性后代个体都是由子囊孢子萌发形成的,且分离自不同的子囊。GUY11的rep-PCR扩增片段中的5个特异性片段的分离比例也都符合1:1的期望值,是按单个位点标记分离的,与已知的结果相吻合,说明有性后代随机群体的质量较高,不存在偏离现象,是一个较为理想的群体。同时,研究结果还得出基于Pot2的rep-PCR方法可以用来快速评价稻瘟病菌有性后代群体的质量。 相似文献
94.
The group of Mid - mini enterprises under mass - economy is the intermediate organization built up by specialized division of work and cooperation. This kind of organization is flexible in operation with high efficiency, but difficult in development of new technology. On the basis of analyzing the character and deficiency of the mid - mini enterprises, in this paper, through providing a framework of information service platform for the mid - mini enterprises to enhance their creative ability and complex competition. The construction and operational strategy of such platform are investigated. Finally a successful case study is given. 相似文献
95.
96.
In this paper,solidifinG ladle of hot metal has been studied from the point ofview of heat transfer.A mathematical model,which is applied to predict the shape and the thicknessof solidifing layer,has been developed. The theoretical calculated results agree with the appliedresults in preduction. The effects of various paramenters,such as the circulating times, the inner-surface temporature of ladle and the different refractories,on the shape and the thickness ofsolidifing layer have been discussed.These are contributed to reduce the solidifing ladle of hot metal. 相似文献
97.
Li Yourong Jin Mingcong Tang Jingwen 《保鲜与加工》1993,(6):46-50
The concept of loss rate of available energy was introduced for evaluating heat exchanger performance. At the same time, the general expression of loss rate of available energy was obtained for heat exchangers of several typical. And NL criteria was recommended for evaluation of the loss rate of available energy of heat exchanger. Then. the influences of inlet temperatures ratio, heat capacity ratio of fluids, the number of heat transfer units and flow patterns of heat exchanger on ,VL criteria were also discussed. 相似文献
98.
Gu Qiankun Tang Yike Yu Youju Xie Zhijiang Liang Xichang 《保鲜与加工》1997,(6):96-102
The dynamic characteristic of active isolation atuator on flexible foundations was studied under the installation of parallel structure and foundation cancellation.The influencing relationships between active isolation force of the different installation ways and passive isolation system and flexible foundation were analyzed and compared.Scientific basis for selecting installation ways of active isolator is provided in this paper. 相似文献
99.
He Yaosan Lin Jingdong Tang Zhuoyao Tong Mingru 《保鲜与加工》1995,(5):7-12
In this paper, the excitation principle and operating characteristics ofasynchronized turbogenerators are analyzed,and adaptive tracing iterative control strategy based on state identification is proposed. In order to apply this control scheme to practice engineering, some primary elements are analyzed and presented for constituting dual-channel automatic excitation control system of asynchronized turbogenerators. Finally, the paper presents the principle structure charts about excitation control system of asynchroized turbogenerators. 相似文献
100.
Load dynamic matching of supply and demand side based on the utilization of urban sewage heat energy
Flowing dynamic characteristics and its temperature dynamic characteristics of a three-level sewage pipe network in Chongqing were analyzed. A mall's building whole year load was simulated. In the adverse condition of wastewater flow, the market construction peak discharge heat was compared with the sewage heat load, the balance between the most adverse flow and the building demand was found. Explored supply and demand matching method between urban sewage and city building load in flow adverse condition to take full advantage of the urban sewage cold and hot resources. the composite system was adeptcd to make up the most unfavorable flow time of sewage average heat load and the construction load difference; it also puts forward principle of load sharing and control strategy aimed at compound system. 相似文献