溴氰菊酯对草鱼鱼种脑AchE及ATP酶活性的影响

本试验研究了溴氰菊酯对草鱼鱼种的急性效应以及溴氰菊酯对草鱼鱼种脑ＡｃｈＥ及ＡＴＰ酶活性的影响。结果表明,溴氰菊酯对规格Ⅰ（平均体重８．２１ｇ）、规格Ⅱ（平均 重６６．６ｇ）草鱼鱼种９６ｈ半数致死浓度（９６ｈＬＣ５０）分别为２．１６μｇ／Ｌ、４．２６μｇ／Ｌ,９５％置信区间分别为１．９３￣２．４２μｇ／Ｌ、３．８２￣４．７５μｇ／Ｌ,离体条件下对草鱼种脑Ｍｇ＾２＋－ＡＴＰ酶活性抑制显著,呈一定的浓度     

乌鲁木齐河下游地区湖泊沉积物的粒度特征与沉积环境

根据乌鲁木齐河下游尾癌湖泊东道海子钻孔沉积物＾１４Ｃ年代和粒度分析,表明沉积物的粒度特征随着入湖水量的波动而发生有规律的变化。最近３０ｋａＢＰ以来该钻孔基本上处于比较稳定的湖泊沉积环境,说明东道海子至少有３万年的发育历史,这也说明古尔班通古特沙漠边缘地带的纵向沙垄具有相当程度的稳定性。最后几十年的湖泊变４干、环境退化过程主要是人类活动造成的。     

Oncolytic Vaccinia Virus Carrying Aphrocallistes vastus Lectin (oncoVV-AVL) Enhances Inflammatory Response in Hepatocellular Carcinoma Cells

Aphrocallistes vastus lectin (AVL) is a C-type marine lectin derived from sponges. Our previous study demonstrated that oncolytic vaccinia virus carrying AVL (oncoVV-AVL) significantly enhanced the cytotoxicity of oncoVV in cervical cancer, colorectal cancer and hepatocellular carcinoma through the activation of Ras/ERK, MAPK/ERK and PI3K/Akt signaling pathways. In this study, the inflammatory response induced by oncoVV-AVL in a hepatocellular carcinoma cell (HCC) model was investigated. The results showed that oncoVV-AVL increased the levels of inflammatory cytokines including IL-6, IL-8 and TNF-α through activating the AP-1 signaling pathway in HCC. This study provides novel insights into the utilization of lectin AVL in the field of cancer therapy.     

江苏省“两沙”海域养殖生产简况及分析

本文主要分析了江苏省＂两沙＂海域养殖生产形势,主要集中分析了紫菜和贝类养殖的生产情况、经济效益情况和面临的挑战,提出了进一步发展的建议。     

新疆生产建设兵团红枣种植布局综合评价研究

红枣是新疆生产建设兵团林果业的重要品种,在新疆和全国占有重要地位。在基于层次分析法的红枣生态适宜性评价、基于综合比较指数的红枣比较优势分析、基于成本收益的市场需求分析基础上,开展集成生态适宜性、综合比较优势、市场需求为一体的红枣种植布局综合评价。研究结果表明,红枣评价为优良的是南疆环塔里木盆地的农一师、农二师、农三师、农十四师,评价结果与生产实际总体相一致,为红枣种植布局优化提供可量化的参考依据。      

带约束拉杆钢管/竹胶板组合空芯短柱的偏心抗压性能

采用冷弯薄壁方型钢管和胶合竹板通过横向约束拉杆和结构胶黏剂复合成顺纹抗压的组合空芯短柱(thin-walled steel tube/bamboo-plywood composite hollow short column with binding bars,SBCCB),采用9个试件研究SBCCB试件的偏心抗压破坏模式、抗压承载力和影响规律。结果表明,SBCCB试件受压破坏形态主要为柱端开胶破坏、横向约束拉杆之间基体胶合界面开胶剥离破坏和胶合竹板局部压屈破坏;SBCCB抗压极限荷载随胶合竹净横截面积增大而提高,随着长细比和荷载偏心率的增大而降低,随空心率增大而增大;约束拉杆可有效延迟SBCCB的开胶剥离破坏,改变屈曲破坏模式,有助于试件抗压承载力的提高;相对于不带约束拉杆试件,SBCCB抗压极限应力提高约17.64%;局部翘曲随约束拉杆间距减小而减小,相对拉杆间距比3.0以下能确保较小的局部翘曲变形。薄壁钢管和胶合竹板能形成较好的复合抗压结构单元,该批试件平均值抗压强度达到18.54 MPa,展现了优异的抗压性能。SBCCB可作为绿色建筑材料广泛应用于现代装配式工程结构,同时拓展竹材的应用途径,实现"以竹代木,以竹代钢",具有很好的工程价值和应用前景。     

不同季节蛋鸡饲料养分浓度的研究Ⅱ.高温期罗曼蛋鸡日粮的优化研究

在高温夏季（１９９３年７月１日～９月３０日，合肥地区，开放式鸡舍，舍温２７．９～３８．７℃，平均３２．３℃），用６种不同配方的日粮饲喂处于相同生境的６组２３～３６周龄罗曼蛋鸡。按罗曼公司推荐的饲养标准配制第１组日粮；对第２～６组日粮养分浓度作适当调整。饲养结果表明：各组间罗曼蛋鸡产蛋率、饲料转化率和产蛋饲料成本有显著（Ｐ＜０．０５）或极显著（Ｐ＜０．０１）差异。其中，第５组鸡产蛋率（８３．９３％）和饲料转化率（４３．６４％）均为最高（Ｐ＜０．０１），比第１组鸡产蛋率（７８．９１％）和饲料转化率（４１．２６％）分别提高６．３６％（Ｐ＜０．０５）和５．７７％（Ｐ＜０．０５）；第５组鸡产蛋饲料成本（２．５５元／ｋｇ）比第１组鸡产蛋饲料成本（２．９２元／ｋｇ）降低１４．５１％（Ｐ＜０．０１）。第５组日粮配方（％）为：玉米６１．０、小麦麸３．０、大豆粕１７．０、鱼粉４．０、菜籽饼４．３、蛋氨酸０．１４、赖氨酸０．０５、骨粉０．８、石粉８．５、磷酸氢钙０．８、食盐０．３１、禽用微量元素添加剂０．１２、罗曼多维素０．０１８。     

中棉所63棉铃对位叶光合生理性能的杂种优势研究

以杂交棉中棉所63及其亲本为材料,在棉铃发育时期对棉铃对位叶的生理和光合性能指标进行测定,探索中棉所63获得高产的杂种优势形成机理。结果表明:1)中棉所63产量优势显著。单株结铃数比两亲本分别高21.52%,43.28%,铃重高2.42%,19.31%,叶面积载铃量高22.39%,22.30%。2)中棉所63在棉铃发育后期棉铃对位叶生理指标表现出显著的杂种优势。棉铃对位叶叶面积分别比亲本高26.60%,59.41%,并且中棉所63棉铃对位叶保持较高水平的SOD活性,使其生理代谢明显提高;与此同时,中棉所63的MDA含量显著低于亲本,细胞膜受损较轻,延缓了对位叶的衰老。3)中棉所63在棉铃发育后期棉铃对位叶光合指标表现出显著的杂种优势。在棉铃发育后期,中棉所63叶绿素含量、SPS活性的高水平,能够维持高效率的光合作用,积累更多的光合产物,从而使中棉所63获得较高的单株结铃数和铃重,最终获得高产。     

A quantitative real‐time PCR assay for detection of Colletotrichum lindemuthianum in navy bean seeds

Bean anthracnose is a seedborne disease of common bean (Phaseolus vulgaris) caused by the fungal pathogen Colletotrichum lindemuthianum. Using seed that did not test positive for the pathogen has been proven to be an effective strategy for bean anthracnose control. To quantify the extent of anthracnose seed infection, a real‐time PCR‐based diagnostic assay was developed for detecting C. lindemuthianum in seeds of the commercial bean class navy bean. The ribosomal DNA (rDNA) region consisting of part of the18S rDNA, 5^.8S rDNA, internal transcribed spacers (ITS) 1, 2 and part of the 28S rDNA of seven races of C. lindemuthianum, 21 isolates of Colletotrichum species and nine other bean pathogens were sequenced with the universal primer set ITS5/ITS4. Based on the aligned sequence matrix, one primer set and a probe were designed for a SYBR Green dye assay and a TaqMan MGB (minor groove binder) assay. The primer set was demonstrated to be specific for C. lindemuthianum and showed a high sensitivity for the target pathogen. The detection limit of both assays was 5 fg of C. lindemuthianum genomic DNA. To explore the correlation between the lesion area and the DNA amount of C. lindemuthianum in bean seed, seeds of the navy bean cultivar Navigator with lesions of different sizes, as well as symptomless seeds, were used in both real‐time PCR assays.     

白骨壤叶水提液清除自由基作用的研究

研究了白骨壤叶的水浸提工艺,并采用邻苯三酚自氧化法、水杨酸法、DPPH法,分别测定了白骨壤叶水提液清除超氧阴离子自由基、羟自由基和DPPH自由基的能力。结果表明,水浸提白骨壤叶的最适工艺条件为:料液比1∶25(g∶mL),温度50℃,时间120min,提取率8.14%。白骨壤叶水提液对超氧阴离子(·O2)-、羟基自由基(·OH)及DPPH·自由基均有一定的清除活性,且与其浓度呈正相关,其IC50分别为1.16,1.42,2.27mg/mL,因此白骨壤叶水提液具有一定的体外抗氧化活性。