Nucleotide sequence and mRNA expression analysis of <Emphasis Type="Italic">β</Emphasis>-actin gene in the orange-spotted grouper <Emphasis Type="Italic">Epinephelus coioides</Emphasis>

The full-length cDNA, encoding the orange-spotted grouper β-actin and spanning 1920 bp including a poly (A) tail, was cloned from its brain cDNA library. The open reading frame encodes a protein of 375 amino acids. Sequence analysis indicated that it contained the typical structural features of cytoplasmic actins, and showed higher homology with other vertebrate β-actin than any other members of the actin family. The partial genomic sequence indicated that the organization of the β-actin gene in the orange-spotted grouper might also be conserved. Northern blot analysis indicated that it was expressed at high levels in the brain, spleen, adipose tissue, ovary, and liver, but at low levels in the gill filament and heart, and at a very low level in the kidney. The expression of β-actin gene in the skeletal muscle was barely detectable. These results indicated that the expression of the orange-spotted grouper β-actin gene showed significant variation in different tissues. Therefore, caution should be taken when using β-actin gene as an internal control in the normalization of gene expression among tissues. Whereas, semi-quantitative RT-PCR analysis indicated that treatment with 17α–methyltestosterone (MT) had little effect on the mRNA expression of β-actin gene in the in vitro incubated hypothalamus, pituitary, and ovary fragments of the orange-spotted grouper, suggesting β-actin can be used as an internal control for RT-PCR analysis of MT effects on gene expression in these tissues.     

当归多糖的生物学功能研究进展

本文针对当归多糖对免疫器官、细胞免疫和体液免疫、调控细胞因子及造血系统的影响和抗肿瘤等生物学功能作一综述。     

布尔山羊和布奶杂一代山羊超数排卵试验

７号布奶杂一代山羊和１号布尔山羊,阴道放置海绵栓同时注射雌二醇２ｍｇ预处理７ｄ,随机分为２组,开始超排处理。第１组每日上午肌注用生理盐水溶解的ＦＳＨ,连续４ｄ,剂量分别为１５０,１００,５０,５０ＩＵ。第２组一次肌注用３００ｇ／ＬＰＶＰ溶解的ＦＳＨ２５０￣３００ＩＵ。两组均于处理后的第３天下午撤除阴道海绵栓,同时皮下注射１５－甲基ＰＧＦ２α０．６ｍｇ,第１组于第４天上午再次注射１５－甲基ＰＧＦ２α     

我国西北地区牧草种子生产现状及存在问题浅析

调查了牧草种子生产现状及存在问题。结果表明：我国西北地区的栽培牧草包括豆科、禾本科、藜科、菊科、苋科、紫草科、十字花科、鸢尾科等９科３６属５３种。其中：豆科牧草１３属２１种；禾本科牧草１６属２５种；其它科牧草７属７种。该区栽培牧草总面积约为１５０万ｈｍ＾２，其中主要豆科牧草占７１％，主要禾本科牧草占１０．５％。主要豆科牧草为紫花苜蓿、沙打旺、草木樨、箭舌豌豆、红豆草、柠条和小冠花。主要禾本科牧草为     

均匀分布法预估未来直径分布改正因子计算公式的研究

本文分析了均匀分布和非均匀分布的进级株数的情形，并利用作图法推导出均匀分布法预估未来直径分布改正因子的计算公式。     

岩溶山区磷石膏堆场重金属迁移对耕地质量的影响及污染风险管控

[目的]探讨磷石膏堆场周边耕地土壤重金属含量的变化、成因及污染风险,为磷化工产业可持续发展及水土环境重金属污染的治理提供科学依据。[方法]通过对贵州省中部磷石膏堆场进行实地调查与采样开展分析和研究。[结果]磷石膏堆场排水、侵蚀冲沟水及洼地积水中Cd,Hg,As的含量都超过农田灌溉水质一类标准(水作)水质限值,特别是Cd。在磷石膏堆场周边50～300 m范围内,玉米地、蔬菜地、水稻土中Cd的含量分别超过农用地土壤污染风险筛选值的4.87～9.43倍、8.83～19.13倍和3.83～13.42倍,堆场周边旱作土主要是Cd,As,Zn的污染风险,而水稻土则主要是Cd,As,Hg,Zn的污染风险;大部分蔬菜地土壤和水稻土中Cd的含量超过农用地土壤污染风险管控值。[结论]天然降雨对露天磷石膏堆场产生的侵蚀及淋溶作用是重金属迁移的主要原因,在地表水流方向上耕地重金属污染风险随着堆场距离的增加而逐步减小。兴修排灌设施及调整种植结构是减少磷石膏堆场周边耕地重金属污染的有效途径。     

One new sesquiterpene from Saussurea laniceps

One new guaiane-type sesquiterpene (1) was isolated from Saussurea laniceps. The structure of the new compound was elucidated by spectroscopic data analysis. The immunomodulatory activity of compound 1 was evaluated. It was found that compound 1 showed significant inhibition for proliferation of murine T cells in vitro.     

Two new benzofuran derivatives with anti-inflammatory activity from Liriope spicata var. prolifera

Two new benzofuran derivatives, 2-(4′-hydroxybenzyl)-5,6-methylenedioxy-benzofuran () and 2-(4′-hydroxybenzoyl)-5,6-methylenedioxy-benzofuran (), along with 8 known compounds were isolated from 60% EtOH extract of the fibrous roots of Liriope spicata var. prolifera. Their structures were elucidated on the basis of extensive spectroscopic analysis. In an in vitro bioactive assay, the two new benzofuran derivatives showed anti-inflammatory activity. Compounds 1 and 2 exhibited significant inhibitory activity against neutrophil respiratory burst stimulated by phorbol 12-myristate 13-acetate (PMA) with IC₅₀ value of 4.15 ± 0.07 and 5.96 ± 0.37 μM, respectively.     

祁连山水源林生态系统结构与功能的研究

在祁连山水源涵养林区,围绕水源林生态系统结构与功能的动态变化,开展了森林小气候、森林水文生态效应的研究。结果表明,祁连山水源涵养林不仅具有拦蓄降水、缩小温差、保持较高土壤湿度等作用,而且具有十分显著的消洪补枯、涵养水源、保持水土的水文生态作用。森林覆被率６５％的流域比３２％的流域年枯水径流量增加２８８７ｍｍ,年洪水径流量减少９８８７ｍｍ;森林土壤贮水２９８１２～３９１９３ｍｍ,草地仅１８２０９ｍｍ;林地很少产生地表径流,草地年土壤流失量１７９３９ｋｇ·ｈｍ－２;高寒山区多年冻土与季冻土致使试验区河川径流系数高达０７３,蒸散较小为１５６５４ｍｍ;多年监测资料显示祁连山林分质量下降,冰川退缩,河川径流逐年减少,生态环境明显恶化,建议各级政府部门高度重视祁连山生态保护问题。     

低丘红壤花生南酸枣间作系统研究III.土壤水分

红壤水分含量随土壤深度而增加为农林间作的水分协同利用提供了可能。但土壤水分动态结果表明农林间作不会改善表层土壤水分状况。南酸枣在干旱季节主要通过吸收深层次土壤水分，与间作花生对表层土壤水分的竞争也不剧烈。与单作南酸枣相比，间作系统中南酸枣种植带土壤水分含量较低。这表明农林间作对土壤水分的影响不是促进间作南酸枣生长、抑制间作花生生长的主要原因。