华北平原冬小麦/夏玉米轮作田能量闭合状况分析

对华北平原冬小麦/夏玉米轮作田连续3a(2003-11-2006-10)涡度相关观测的能量闭合状况进行综合分析,并探讨下垫面对能量闭合程度的影响.结果表明,能量平衡比率(EBR)日变化规律明显,以昼夜交替时波动最大,下午略高于上午,白天能量闭合状况明显优于夜间.将湍流能的相位提前0.5h,湍流通量和有效能的匹配度及能量闭合程度均得到提高.白天EBR呈秋冬高(分别为0.98和0.94)、春夏低(分别为0.85和0.70)的季节变化特征,EBR的波动幅度依次为:夏＞秋＞冬＞春.下垫面状况对地表能量闭合程度影响显著,裸地阶段、小麦季和玉米季的年均白天EBR分别为1.11、0.94和0.74,小麦季的能量闭合状况优于玉米季;EBR波动幅度依次为:裸地阶段＞玉米季＞小麦季.观测期间,白天EBR年均值范围在0.82 ～0.97,平均0.89,较好地满足了农田通量观测对数据质量的要求.     

工作压力对滴灌管迷宫流道灌水器水力性能的影响

降低滴灌系统灌水器工作压力有望成为减少滴灌系统能耗以及运行费用的一种有效途径,但目前低压灌水器还十分少见。基于此,选取了国内应用较为广泛的5种典型迷宫流道灌水器,分析了不同工作压力区间对灌水器水力性能及消能特征的影响。结果表明：5种灌水器在低压条件下运行对于灌水器流量系数Kd和流态指数x具有一定影响,但对于流态指数x的影响未达显著水平。同一流道类型的灌水器流量系数Kd与无量纲数A/L2呈显著的线性相关关系,不同流道类型之间差异显著。5种灌水器流道内流态为紊流,未发生流态转捩行为,采用常规管道流态转捩雷诺数2200去判断流道内流态是不合适的。     

Characterization of geosmin as source of earthy odor in different aroma type Chinese liquors

Earthy odor is one of the most frequent and serious causes for the aroma deterioration in Chinese liquor, which causes a dirty and dusty impression. The odor in Chinese liquor is similar to that of rice husk, one kind of auxiliary material widely used as a filler in the distillation process. So it is experientially hypothesized that such odor may derive from rice husk. In this paper, the gas chromatography-olfactometry (GC-O) technique and gas chromatography-mass spectrometry (GC-MS) were used to discover and identify the characteristic odoriferous zone of Chinese liquor marked by earthy odor. Geosmin was found to be responsible for this odor. The levels of the compound in ten bottled liquors and thirty liquors aging for different years belonging to four different aroma types were determined by the optimized headspace-solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) method. Quantitative analysis of bottled liquor revealed the presence of geosmin in all aroma type liquors with concentrations ranging from 1.10 μg/L to 9.90 μg/L, except for strong-aroma type liquor. Meanwhile in the aged liquors belonging to the same aroma type, geosmin was detected with significant concentrations and high odor activity values (OAVs) during different years of aging. However, geosmin was not detected in steamed rice husk nor in nonsteamed rice husk, which suggests that rice husk is not the origin of earthy odor in Chinese liquor, and there may be another origin of it during the brewing process.     

Screening of danofloxacin residue in bovine tissue by terbium-sensitized luminescence on c18 sorbent strips

Danofloxacin (DANO) residue in bovine muscle was screened at 200 ng/g by terbium-sensitized luminescence (TSL) directly measured on 10 × 6 mm C18 sorbent strips. The analyte was first adsorbed on sorbent surface by immersion in defatted homogenates. After reagent application and desiccation, TSL was directly measured on sorbent surfaces at λ(ex) = 273 nm and λ(em) = 546 nm. The luminescence intensity was linearly dependent on DANO concentration in the 0-1000 ng/g range (R(2) = 0.9967). A threshold was established at x(200) - 3σ(200), where x(200) and σ(200) are the mean and standard deviation, respectively, of the DANO signals at 200 ng/g. Among 48 blind samples randomly fortified at 0-1000 ng/g, 45 were screened correctly and 3 negative samples were presumed positive. This simple screening protocol has the potential to significantly reduce sample numbers and hence improve sample throughput and save assay costs.     

Identification of new strawberry sulfur volatiles and changes during maturation

Two Florida strawberry cultivars, 'Strawberry Festival' and 'Florida Radiance', were harvested at five fruit developmental stages (white, half red, three-quarter red, full ripe, and overripe) at four harvest dates. A static headspace solid-phase microextraction (SPME) sampling technique coupled with gas chromatography (GC) using pulsed flame photometric detection (PFPD) was employed to measure 16 sulfur volatiles in these strawberries. A total of 7 sulfur volatiles have been previously reported, and 9 are reported for the first time in strawberries. Newly identified sulfur volatiles include methyl thiopropionate, ethyl thiobutanoate, methyl thiohexanoate, methyl (methylthio)acetate, ethyl (methylthio)acetate, methyl 2-(methylthio)butyrate, methyl 3-(methylthio)propionate, ethyl 3-(methylthio)propionate, and methyl thiooctanoate. Identifications were based on matching sulfur peak linear retention indexes (LRIs) of unknowns with authentic standards and gas chromatography-mass spectrometry (GC-MS) data. Concentrations were determined using both internal and external standards. Most sulfur volatiles increased with increasing maturity, with only concentrations of hydrogen sulfide and methanethiol remaining relatively consistent at all five stages. At the white and half red stages, most sulfur volatiles consisted of various alkyl sulfides. At three-quarter red (commercial ripe), full ripe, and overripe stages, the majority of sulfur volatiles consisted of sulfur esters. Most sulfur volatiles increased dramatically between the commercial ripe, full ripe, and overripe stages, increasing as much as 100% between full ripe and overripe. Principal component analysis indicated that sulfur volatiles could be used to distinguish overripe from full ripe and commercial ripe berries.     

磷在潮土肥际微域中的迁移和转化

采用室内土柱实验研究了磷在潮土肥际微域中的迁移和形态转化。结果表明,磷在潮土中的移动距离很短,经过7 d和28 d的迁移,磷的迁移距离仅为8～14 mm。施入的磷主要固定在紧靠施肥点2 mm土体范围内,随距施肥点距离的增加,土壤水溶性磷、酸溶性磷和有效磷的含量呈指数曲线迅速降低。磷在施肥后7 d内的迁移量较大,迁移进入土壤的磷绝大部分被吸附固定。施肥量对不同形态磷在肥际微域中的迁移量产生显著影响;但培养时间只明显影响了水溶性磷的迁移量。     

Identification and quantification of cyclolinopeptides in five flaxseed cultivars

Cyclolinopeptides are a group of naturally occurring hydrophobic cyclic peptides found in flaxseed and flax oil that have immunosuppressive activity. This study describes the measurement of flaxseed cyclolinopeptide concentrations using an internal standard HPLC method. In addition, the concentration of cyclolinopeptides in the seed of Canadian flax cultivars grown at two locations over two years is reported. The data are consistent with the formation of flaxseed cyclolinopeptides from two ribosome-derived precursors. Each precursor protein includes the sequences corresponding to three cyclolinopeptides from which those cyclolinopeptides are presumably derived by precursor processing. The concentrations of cyclolinopeptides C and E, which are encoded by the same gene sequence, are highly correlated, and the concentrations of cyclolinopeptides D, F, and G, which are encoded by a second gene sequence, are also highly correlated. The strong correlation between the cyclolinopeptides arising from the same gene may prove to be important in understanding how peptide concentration is controlled. Additional research may lead to approaches to improve flax either as a platform for peptide production or as a source of oil with improved drying properties and flavor.     

基于多源遥感数据的综合干旱监测模型构建

在全球气候变化越来越复杂的大背景下,准确监测华北粮食主产区的旱情对区域农业生产有重要的指导意义。以往的遥感干旱监测方法多侧重于监测土壤或植被等单一干旱响应因子,反映综合信息的能力较差,为此该研究使用中分辨率成像光谱仪(moderate-resolution imaging spectroradiometer,MODIS)、热带降水测量计划(tropical rainfall measuring mission,TRMM)卫星等多源遥感数据,在综合考虑干旱发生发展过程中的土壤水分胁迫、植被生长状态和气象降水盈亏等因素的基础上,利用空间数据挖掘技术,构建综合干旱监测模型,并以山东省为例进行了试验验证。结果表明,模型监测出山东省近年来所经历的重大干旱过程与实际旱情一致,模型输出的旱情指标-综合干旱指数(synthesized drought index,SDI)与小麦的标准化作物单产变量的相关系数均大于0.7(P0.05);在小麦和玉米的生长期,综合干旱指数与作物受灾面积的相关系数在-0.67～-0.85之间,与标准化降水指数(standardized precipitation index,SPI)的相关系数在0.44～0.67之间,且通过了P0.01的极显著检验(3月份除外)。研究结果为综合评估区域干旱提供了一种新的方法。     

高速插秧机钵体毯状苗纵向送秧装置的设计与试验

基于现有高速插秧机平台,改进设计适用于钵体毯状苗的纵向送秧装置。对钵体毯状苗的插秧特点进行分析。针对钵体毯状苗对纵向送秧装置的要求,对纵向送秧装置进行了详细的理论分析和设计。通过Solidworks建模并对装置的工作状态进行虚拟仿真。设计加工样机进行试验分析,试验结果表明,多次纵向送秧误差均在2 mm以内,且不存在累积误差,故满足设计要求。保证了机器在高速工作状况下,纵向送秧的精度和非送秧过程时秧苗的纵向位置固定。该文设计的纵向送秧装置使得钵体毯状苗在插秧机平台上的纵向送秧:精准、稳定、可靠。为钵体毯状苗实现高速机械插秧提供了可靠的设计方案和理论依据。     

蜡样芽孢杆菌及其生物被膜对有机酸及乙醇的抗性比较

为比较蜡样芽孢杆菌及其生物被膜对环境压力的抗性,该文主要研究了4种有机酸（乙酸、柠檬酸、乳酸和苹果酸）和乙醇对蜡样芽孢杆菌及其生物被膜存活率的影响。结果表明：生物被膜态蜡样芽孢杆菌对乙酸的抗性高于浮游态菌。扫描电镜结果显示,经乙酸处理后,浮游态蜡样芽孢杆菌的细胞表面严重受损,而生物被膜态菌的表面形态未发生明显变化。在柠檬酸、乳酸、苹果酸和乙醇存在的条件下,生物被膜态蜡样芽孢杆菌比浮游态菌表现出更强的压力抗性,特别是在高浓度（有机酸16%～20%,乙醇50%～60%）的情况下,该现象尤为显著。因此,在食品工业中控制被膜态蜡样芽孢杆菌对预防和阻止食品腐败非常重要。该研究结果为实际生产中有效控制蜡样芽孢杆菌及其生物被膜的形成提供参考。