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101.
为研究As2O3对实验兔生长和繁殖性能的影响,选择年龄和体重相近的40只实验兔(32只母兔,8只公兔)随机分成4组.对照组饲喂不含As2O3的基础日粮,试验Ⅰ组、试验Ⅱ组和试验Ⅲ组分别饲喂含As2O3 0.5mg/kg、1.0mg/kg和1.5mg/kg的日粮.生长发育和繁殖性能的测定结果显示,试验Ⅰ组与对照组相比,差异不显著(P>0.05),试验Ⅱ组性能表现优于对照组,差异显著(P<0.05),试验Ⅲ组出现中毒症状.本实验表明,适量的As2O3对实验兔生长和繁殖具有促进作用. 相似文献
102.
仔猪肠毒血症又名仔猪传染性坏死性肠炎、猪梭菌性肠炎,俗称仔猪红痢。主要发生于1周龄内的新生仔猪,以泻出红色带血稀粪为特征。淮安市楚州区某乡村连续发生多起60日龄左右仔猪以衰竭、瘫痪、昏迷、急性死亡为特征的病例。经流行病学调查、临床症状、尸体剖检、实验室检验,确诊为产气荚膜芽孢梭菌引起的急性肠毒血症。 相似文献
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Hall JA Wander RC Gradin JL Du SH Jewell DE 《American journal of veterinary research》1999,60(3):319-327
OBJECTIVE: To determine effect of diets with variable n-6-to-n-3 fatty acid (FA) ratio on CD4+ and CD8+ T-lymphocyte subpopulations, and on results of routine laboratory analyses (CBC and total WBC count, serum biochemical analyses, and urinalysis). ANIMALS: 20 healthy, aged (9.5 to 11.5 years old) female Beagles. PROCEDURE: Dogs were fed 1 of 3 diets that contained 6% fat by weight but differed in amounts of n-6 and n-3 FA. For 11 weeks, 6 dogs were fed a low concentration of n-3 FA (ratio, 31:1), 7 were fed a medium concentration (5.4:1), and 7 were fed a high concentration (1.4:1). Preprandial blood and urine samples were collected before beginning the study and at 8 weeks for evaluation of laboratory variables. Before and at 3, 6, and 8 weeks during the study, blood was drawn for total WBC and lymphocyte counts and for characterization of T-cell subpopulations. At 8 and 10 weeks, dogs were vaccinated with keyhole limpet hemocyanin suspension. Blood was drawn 4 days after each vaccination, and lymphocytes were isolated for flow cytometry. Effects of diet and vaccination on each variable were determined. RESULTS: After vaccination, total lymphocyte count increased and CD4+ T lymphocyte count and the CD4(+)-to-CD8+ ratio decreased in dogs consuming the diet with n-6-to-n-3 FA ratio of 1.4:1. CONCLUSION: Feeding a diet with n-6-to-n-3 FA ratio of 1.4:1 had significant effects on CD4+ T lymphocytes in healthy, aged Beagles after vaccination. 相似文献
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The preparation of the antigen, details of the reagents, the titration of the antispecies conjugates and the execution of the indirect fluorescent antibody test are described. The sensitivity and specificity of the test and its applicability to the detection of antibodies to Cowdria ruminantium are recorded. The test is both highly specific and sensitive and can be applied to a wide range of studies on heartwater, including epidemiology, determination of the C. ruminantium infection rate of Amblyomma ticks and the evaluation of immunization against heartwater. The test can also be used to detect antibodies to the heartwater agent in the sera of game. 相似文献
109.
Mannheimia haemolytica leukotoxin is produced during the logarithmic growth phase in submerged culture in RPMI 1640 medium with and without the addition of foetal calf serum or albumin. In order to establish a pattern of optimal leukotoxin production in small volumes in submerged cultures and to define some parameters involved, two high leukotoxin producing Mannheimia haemolytica strains were grown in RPMI 1640 medium containing either FCS or BSA. The cell growth and leukotoxin production abilities of each strain were determined concomitantly every hour in RPMI 1640 medium containing each of the additives over a time period of 6 h. The growth performance of three dilutions of a standardized seed culture inoculum prepared with each of the cultures and additives were simultaneously compared with each other using the above parameters. The different seed culture inoculum dilutions had a definite effect on the time and quantity of leukotoxin production. Both strains demonstrated peak leukotoxin production after 4 h of active growth. The addition of albumin to both isolates gave slightly increased leukotoxin levels, and both showed that the peak leukotoxin was not associated with peak cell concentration. Obvious quantitative differences in the ability of different M. haemolytica strains to produce leukotoxin were noted. Strain 12296 produced optimal leukotoxin concentration from the medium (1/25) dilution of the seed culture inoculum after 4 h, whereas strain 1/10 produced the same concentration with the low (1/5) dilution seed culture inoculum, possibly reflecting the superior production ability of the first strain. However, each strain of M. haemolytica appeared to have its own specific logarithmic cell growth and leukotoxin production pattern. The peak cell density of M. haemolytica grown in submerged RPMI 1640 culture medium cannot be used as an indication of optimal leukotoxin levels. 相似文献
110.
Parameters for the determination and evaluation of heat stress in dairy cattle in South Africa 总被引:2,自引:0,他引:2
Du Preez JH 《The Onderstepoort journal of veterinary research》2000,67(4):263-271
Not all parameters are trustworthy and practical to use as parameters to determine heat stress in dairy cattle. The temperature-humidity index (THI) is still the best, simplest and most practical index (parameter) for measurement of environmental warmth which cause heat stress in dairy cattle. It is practical, easy to determine and relatively trustworthy to use body temperature and respiratory rate as parameters to determine heat stress in dairy cattle. These physiological parameters must always be used together with THI values to determine and evaluate heat stress in dairy cattle. For practical purposes, plasma cortisol concentration and milk composition cannot be used as parameters to determine heat stress in dairy cattle although good indications of acute or chronic heat stress can be obtained. Vanillic acid is a break-down product of adrenalin found in milk, but before its concentration in milk can be used as an indicator/parameter of heat stress in dairy cows, more about the pharmacodynamics of adrenaline in the milk has to be known. Selection and breeding of dairy cows on the basis of their adaptibility to heat stress using the most practical heat stress parameters will ensure that their offspring will have superior performance in the prevailing environmental conditions. 相似文献