Generation of Salmonella ghost cells expressing fimbrial antigens of enterotoxigenic Escherichia coli and evaluation of their antigenicity in a murine model

Salmonella Typhimurium ghost cells expressing K88ab, K88ac, K99, and FasA fimbriae of enterotoxigenic Escherichia coli (ETEC) in their envelopes were constructed. The genes encoding the fimbriae were individually cloned into an expression plasmid, pMMP81, carrying the asd gene, which was subsequently electroporated into the Δasd S. Typhimurium mutant. Plasmid pJHLP99, carrying the phiX174 lysis gene E, was also subsequently electroporated into the Salmonella mutant. The presence of the individual fimbriae on the ghost cells was examined by Western blot analysis. Forty BALB/c mice were equally divided into 2 groups of 20 mice each. Group A mice were intramuscularly vaccinated with a mixture of the 4 ghost cells expressing the individual fimbriae. The group B mice were inoculated with sterile phosphate-buffered saline as a control. The antigen-specific serum IgG concentrations were significantly higher in group A than in group B from week 2 until week 6 after inoculation. In addition, the antigen-specific IgA concentrations in fecal samples were significantly higher in group A than in group B at week 2 after inoculation. A large difference between the groups in the number of antigen-specific IgA-secreting cells in the small intestine was observed by immunohistochemical study. Also, the splenic lymphocyte proliferative responses were significantly greater in group A than in the control mice. These results suggest that vaccination with our Salmonella ghost cells can induce both humoral and cell-mediated immune responses and that the increased number of antigen-specific IgA-secreting cells in the small intestine may be correlated with the elevated fecal IgA immune response.     

梳脱式联合收割机脱粒与输送装置模糊控制系统

建立了梳脱式联合收割机脱粒与输送装置的动力学模型，应用Fuzzytoo1box设计了模糊控制器。当作物属性变化等因素而引起该装置的工作负荷发生较大变化时，模糊控制器能够对机组前进速度傲出相应的调整，使被控量迅速恢复到设定值附近，且能保持稳定，有利于发挥机器的最大的工作潜能，提高机器的可靠性，降低机器故障率。     

新城疫病毒La Sota株HN基因的克隆与序列分析

旨在从分子生物学角度进一步研究新城疫病毒La Sota株HN基因,探究NDV La Sota HN基因的遗传变异情况。研究以试验室冻存的p NDV-HN为模版,根据Gen Bank中登录的NDV La Sota株序列设计引物扩增HN基因,将其克隆到pMD18-T载体后进行鉴定,对鉴定正确的重组质粒p MD-NDV-HN进行测序分析。应用生物信息学软件对新城疫病毒Lasota株HN基因进行系统进化树分析、氨基酸序列同源性分析、糖基化位点、蛋白结构跨膜区分析及磷酸化位点预测分析。核苷酸序列测定结果表明:HN基因的序列长度为1743bp,该基因的开放阅读框(Open Reading Frame,ORF)总长为1716 bp,编码571个氨基酸。生物信息学表明:试验获得的HN基因具有6个潜在糖基化位点及12个半胱氨酸残基,第5个潜在糖基化位点(508-510 aa)发生缺失及第123位半胱氨酸残基被色氨酸残基取代。La Sota株HN基因编码的蛋白质中有29个丝氨酸、10个酪氨酸和16个苏氨酸可能成为蛋白激酶磷酸化位点。将试验获得的La Sota株HN基因序列和推导的氨基酸序列与新城疫毒株的HN基因相应序列比较后发现,它们的核苷酸序列同源性和氨基酸序列同源性最高均可达到99%,表明HN基因在种属间具有较高的保守性。研究为新城疫病毒的分子病毒学研究奠定了基础。     

农民生计多样性影响因素调查分析 ——以丰都县为例

丰都县农民生计多样性在整个西部地区比较具有代表性，但对于影响农民生计多样性的因素一直缺乏实证研究。该次调查采用参与性农村评估法（PRA），对丰都县名山镇（两汇口、古家店、鹿鸣寺）、社坛镇（三桥、德盛、干坛）、武平镇（殷家坝、坝周村）的农民生计方式及影响农民生计多样性的因素进行调查研究。研究表明，影响农民生计多样性的因素大致包括：自然资源、环境、人力资本、物质基础、社会关系、国家政策等方面     

Controlling Techniques of Channel Sharing in HFC Network

The techniques of sharing a channel in HFC are original with regard to those of Ethernet and Ring. In this paper, the analysis is stressed on the method of the upstream bandwidth allocation, the collision resolving, and the relationship among them, beginning with MAP management message. The MAP is a complex frame, in which the messages of ranging opportunity needed by the CM at the access beginning, bandwidth allocation contention, bandwidth allocation and collision detection are included. The access way for a user is explained after the frame format and functions of a MAP are illustrated. Finally, upstream bandwidth allocation, the cause of collision, algorithm and the process to solve the collision and so on are studied.     

A Study on the Method of Time-Resource Optimization of Engineering Projects

This paper investigates the coordinated operation of the progress planning with the resource planning when carrying out engineering projects and puts forward a method of "RSM" under the resource limit conditions. Then, the "RSM" was improved to avoid complicated calculation. This method not only reduces the calculating work by hand but saves computer's memory as well, making the time-resource optimization work more quick and convenient.     

A Thermodynamic Criteria for Evaluation of Heat Exchanger Performance

The concept of loss rate of available energy was introduced for evaluating heat exchanger performance. At the same time, the general expression of loss rate of available energy was obtained for heat exchangers of several typical. And NL criteria was recommended for evaluation of the loss rate of available energy of heat exchanger. Then. the influences of inlet temperatures ratio, heat capacity ratio of fluids, the number of heat transfer units and flow patterns of heat exchanger on ,VL criteria were also discussed.     

Unicity Theorem of Meromorphic Functions

ln this paper,we firstly in vestigated the un icity properties of meromorphic functions with three common values,extended the results of H. Ueda,Yi Hongxun.Moreover,wealso improved the results of K,Shibazaki,Yi Hongxun and C,C,Yang,Hua xinhou and some others     

Principle and Key Technology of Full Automatic Vicat Apparatus

In this paper the working principle and performance character of both the manual and automatic Vicat apparatuses in existence are compared and analyzed,and their existing weakness and the urgent problem to be solved are pointed out.On this basis,adopting the technology of mechatronics and modern sensors,a new sort of full automatic Vicat apparatus that can fully satisfy the national standard in testing the setting time of the cement has been developed.This apparatus can be operated expediently with high precision,the test results are to be analyzed and computed automatically.This paper presents the working principle,performance character,key technology and prospect of application for the full automatic Vicat apparatus.