全文获取类型
收费全文 | 20544篇 |
免费 | 229篇 |
国内免费 | 530篇 |
专业分类
林业 | 4147篇 |
农学 | 1982篇 |
基础科学 | 462篇 |
3455篇 | |
综合类 | 1843篇 |
农作物 | 2296篇 |
水产渔业 | 1940篇 |
畜牧兽医 | 1757篇 |
园艺 | 1263篇 |
植物保护 | 2158篇 |
出版年
2024年 | 12篇 |
2023年 | 66篇 |
2022年 | 183篇 |
2021年 | 228篇 |
2020年 | 205篇 |
2019年 | 200篇 |
2018年 | 2892篇 |
2017年 | 2898篇 |
2016年 | 1317篇 |
2015年 | 277篇 |
2014年 | 221篇 |
2013年 | 241篇 |
2012年 | 1056篇 |
2011年 | 2360篇 |
2010年 | 2341篇 |
2009年 | 1509篇 |
2008年 | 1522篇 |
2007年 | 1801篇 |
2006年 | 243篇 |
2005年 | 273篇 |
2004年 | 189篇 |
2003年 | 229篇 |
2002年 | 134篇 |
2001年 | 89篇 |
2000年 | 132篇 |
1999年 | 90篇 |
1998年 | 90篇 |
1997年 | 66篇 |
1996年 | 64篇 |
1995年 | 67篇 |
1994年 | 43篇 |
1993年 | 71篇 |
1992年 | 45篇 |
1991年 | 32篇 |
1990年 | 27篇 |
1989年 | 25篇 |
1988年 | 27篇 |
1987年 | 9篇 |
1986年 | 5篇 |
1985年 | 3篇 |
1983年 | 3篇 |
1982年 | 2篇 |
1977年 | 4篇 |
1972年 | 1篇 |
1969年 | 1篇 |
1968年 | 4篇 |
1967年 | 1篇 |
1956年 | 2篇 |
1950年 | 1篇 |
1935年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 222 毫秒
11.
芝麻的核型与系统演化 总被引:1,自引:0,他引:1
对10份粒色不同的栽培和野生芝麻的染色体组型研究表明,栽培芝麻染色体数目一致,核型基本相同:2n=26=12m+12Sm+2St随着粒色的变浅,核型的不对称性加强,有两对随体染色体。野生芝麻核型与栽培芝麻相似:2n=26=12m+12Sm(4SAT)染色体数目有2n=26,2n=32,2n=58或2n=64.芝麻可能是由对称核型向不对称核型演化,其趋势为:野生(2n=26)-黑粒-褐袜-黄粒-白粒 相似文献
12.
选择母岩、土壤类型、土地利用和降雨量 4个生态因子 ,应用半定量化方法对其进行类目划分和权重 ,综合评价了福建省土壤对酸沉降的相对敏感性 ,并应用地理信息系统进行了区划 .由于福建省风化缓慢的硅质岩所占的比例较大 ,土壤大多呈酸性至微酸性反应 ,降水丰富 ,加之针叶林面积大 ,所以全省有 60 %以上面积的土壤显示出较大的敏感性等级 (5~ 7级 ) 相似文献
13.
本文用6个亲本,按(1/2)n(n-1)双列杂交法配制15个杂交组合,对辣椒TMV、CMV、疫病、疮痂病抗性进行了配合力分析。结果表明,不同亲本、不同病害抗性的一般配合力效应和不同杂交组合的特殊配合力效应差异较大,疫病抗性显性方差所占份量较大,加性方差所占的份量较小;CMV和疮痂病抗性是加性方差所占份量较大,显性方差所占份量相对较小;TMV抗性介于二者之间。不同病害抗性间所有一般配合力的相关系数均未达到显著水平;特殊配合力是CMV与疮痂病阃的相关达到了极显著水平,TMV与CMV间和疫病与疮痂病间的相关系数达到显著水平。 相似文献
14.
A. Schots J. De Boer A. Schouten J. Roosien J. F. Zil Verentant H. Pomp L. Bouwman-Smits H. Overmars F. J. Gommers B. Visser W. J. Stiekema J. Bakker 《European journal of plant pathology / European Foundation for Plant Pathology》1992,98(2):183-191
Engineering resistance against various diseases and pests is hampered by the lack of suitable genes. To overcome this problem we started a research program aimed at obtaining resistance by transfecting plants with genes encoding monoclonal antibodies against pathogen specific proteins. The idea is that monoclonal antibodies will inhibit the biological activity of molecules that are essential for the pathogenesis. Potato cyst nematodes are chosen as a model and it is thought that monoclonal antibodies are able to block the function of the saliva proteins of this parasite. These proteins are, among others, responsible for the induction of multinucleate transfer cells upon which the nematode feeds. It is well documented that the ability of antibodies to bind molecules is sufficient to inactivate the function of an antigen and in view of the potential of animals to synthesize antibodies to almost any molecular structure, this strategy should be feasible for a wide range of diseases and pests.Antibodies have several desirable features with regard to protein engineering. The antibody (IgG) is a Y-shaped molecule, in which the domains forming the tips of the arms bind to antigen and those forming the stem are responsible for triggering effector functions (Fc fragments) that eliminate the antigen from the animal. Domains carrying the antigen-binding loops (Fv and Fab fragments) can be used separately from the Fc fragments without loss of affinity. The antigen-binding domains can also be endowed with new properties by fusing them to toxins or enzymes. Antibody engineering is also facilitated by the Polymerase Chain Reaction (PCR). A systematic comparison of the nucleotide sequence of more than 100 antibodies revealed that not only the 3′-ends, but also the 5′-ends of the antibody genes are relatively conserved. We were able to design a small set of primers with restriction sites for forced cloning, which allowed the amplification of genes encoding antibodies specific for the saliva proteins ofGlobodera rostochiensis. Complete heavy and light chain genes as well as single chain Fv fragments (scFv), in which the variable parts of the light (VL) and heavy chain (VH) are linked by a peptide, will be transferred to potato plants. A major challenge will be to establish a correct expression of the antibody genes with regard to three dimensional folding, assembly and intracellular location. 相似文献
15.
16.
17.
18.
Shohei MATSUURA Shigeru HOSHINO Hideaki HAYASHI Tetsuyuki KOHGUCHI Kyoji HAGIWARA Toshihiro OMURA 《Journal of General Plant Pathology》2002,68(1):99-102
DAS-ELISA proved to be reliable enough to detect a latent infection by Tomato spotted wilt virus (TSWV) in asymptomatic stock plants of chrysanthemum. A high density of Frankliniella occidentalis, the predominant vector, in the presence of latently infected stock plants resulted in a high incidence of disease in the chrysanthemum
production field. The incidence of disease was low when the vector thrips were not abundant in spite of the presence of latently
infected stock plants. These results suggest that an infestation of the vector thrips causes severe secondary spread of TSWV
originating from latently infected stock plants in chrysanthemum production fields.
Received 27 July 2001/ Accepted in revised form 27 November 2001 相似文献
19.
20.