凡纳滨对虾精氨酸激酶的分离纯化及性质研究

经过CM-纤维素批量层析、Separdex G-100柱层析、DEAE_纤维素柱层析等步骤,从凡纳滨对虾肌肉组织分离得到精氨酸激酶,经SDS-PAGE检测达到电泳纯,分子量约为40kDa.对该酶的性质进行分析结果表明,精氨酸激酶的最适作用温度为55℃,当温度高于65℃时,酶活力显著下降;pH 8时酶活力较高,低浓度的精氨酸对酶活力有促进作用,高浓度时表现抑制作用,而底物类似物精胺和氨基胍则对酶促反应表现出完全的抑制.NaCl,KCl对酶的活力具有促进作用,低浓度(10 mmol·L-1)MgCl2对酶活力表现出激活作用,而CuCl2与MnCl2则表现出完全抑制酶活力,CaCl2与ZnCl2在低浓度时对酶活力无明显影响,但是随着浓度升高,对酶具有抑制作用.     

硇洲岛周围水域虾拖网虾渔获组成

根据2005年渔业资源监测船生产调查资料和现场取样数据,运用数理统计方法分析了虾拖网虾渔获组成、生物学特征以及月份变化等。结果表明:硇洲岛周围水域虾拖网捕获的虾类主要是沿岸性底栖虾类,渔获中优质虾种少,幼虾比例高,墨吉明对虾、日本囊对虾、哈氏仿对虾是虾拖网渔业的主捕虾种,虾渔获组成存在明显的月份变化。为合理利用虾类资源和促进虾渔业可持续发展,应根据虾类特性开发适宜广东沿海虾拖网的选择性捕捞技术,减少幼虾兼捕量。     

货车气压制动系统键图分析仿真

针对现有的货车制动系统,应用键图法建立数学模型,并进行模拟仿真,为制动系统的研究提供了理论基础.     

数控仿真软件在教学中的应用特点分析

分析数控仿真软件在数控加工课程教学中的应用特点,并针对其优缺点提出了相应的措施,以期在教学过程中能充分发挥分析数控仿真软件的优点,克服其缺点,提高教学效果,使学生尽快掌握数控机床的操作技能.     

农村电网标准化作业管理系统的设计

以C/S和B/S结构为基础,应用UML技术设计农村电网标准化作业管理系统。介绍标准化作业的管理流程,阐述该系统的开发环境、架构和功能,设计了系统用例,并重点介绍作业指导书的设计。经运行实践表明,应用该标准化作业管理系统可实现标准化作业管理的科学化和规范化。     

金秋梨栽培技术

介绍了金秋梨壮苗培育,科学建园,土壤管理和施肥技术以及整形修剪,花果管理和病虫害防治等栽培技术。     

葡萄枝条中淀粉粒形态结构与抗寒力的关系

扫描电镜观察结果表明,越冬期间葡萄枝条组织细胞中淀粉粒的大小、积累数量和转化动态规律与抗寒力密切相关,主要表现为抗寒种类或品种的淀粉粒小,积累早而数量多,水解晚但速度快而较彻底;同一品种采用嫁接栽培可明显增加枝条中淀粉的积累数量,有利于枝芽成熟和越冬。     

赤霉素和细胞分裂素调控苹果果实早期生长发育机理的研究

采用流式细胞术（ＦＣＭ）及酶联免疫法（ＥＬＩＳＡ）从细胞学和激素生理学的角度研究了ＧＡ４／７、ＢＡ及ＣＰＰＵ调节苹果果实早期发育的可能机理。ＧＡ４／７（５０ｍｇ／Ｌ）抑制了苹果果肉细胞分裂,未提高果实内源细胞分裂素（ＺＲｓ,ｉＰＡ）含量推测它主要通过促进工而促进果实生长,ＢＡ（５０ｍｇ／Ｌ）和ＣＰＰＵ（１２．５ｍｇ／Ｌ）可以显著促进苹果幼果的细胞分裂,ＣＰＰＵ的细胞分裂素活性较ＢＡ高,ＢＡ和ＣＰＰ     

Effects of oxidative stress and inflammatory response on kidney injury in mice with hyperthyroidism

AIM To explore the effects of oxidative stress and inflammatory response on kidney injury induced by hyperthyroidism in mice. METHODS Forty male Kunming mice were randomly divided into control group （n=20） and L-thyroxine （T4） group （n=20）. The mice in T4 group were intraperitoneally injected with T4 diluent at a dose of 1 mg/kg to induce hyperthyroidism, and those in control group were injected with normal saline of the same volume. After 7 weeks, the mice were weighed and dissected, the kidneys were removed and weighed, and the length of tibia was also measured. The activity of superoxide dismutase （SOD） and the content of malondialdehyde （MDA） in the kidney tissues were detected. The pathological changes of the kidney tissues were observed by HE staining. The levels of 4-hydroxynonenal （4-HNE）-modified proteins, interleukin-1 receptor-associated kinase 1 （IRAK1） and tumor necrosis factor receptor-related factor 6 （TRAF6） were determined by Western blot and immunohistochemistry. RESULTS Compared with control group, the body weight of the mice was decreased, while the kidney size and weight were increased significantly in T4 group. In addition, the ratios of kidney weight/body weight and kidney weight/tibia length were also increased （P<0.05）. In T4 group, the renal tubules were enlarged, and the epithelial cells of renal tubules were swollen and exfoliated, with vacuolar degeneration. Furthermore, reduced SOD activity, and increased MDA content and 4-HNE-modified proteins were found in T4 group, all of which were related to oxidative stress （P<0.05）. The levels of inflammation-related proteins IRAK1 and TRAF6 were significantly increased in T4 group （P<0.05）. CONCLUSION Excessive T4 may lead to kidney hypertrophy and injury in mice, and the mechanism may be related to oxidative stress and inflammatory response.