利用SSR和SRAP标记分析花椰菜自交系的遗传多样性

为选育优质花椰菜新品种,指导种质资源引进和利用,本研究采用简单重复序(simple sequence repeat,SSR)标记和相关序列扩增多态性(sequence-related amplified polymorphism,SRAP)标记对38份花椰菜自交系进行了遗传多样性分析,分别从48对SSR引物、48对SRAP引物中各筛选出4对有效引物。4对SSR引物扩增的总条带数为47个,多态性条带为39个,平均多态性比率达83.0%;4对SRAP引物扩增的总条带数为86个,多态性条带为51个,平均多态性比率为59.3%,该结果显示花椰菜自交系间具有较丰富的遗传多样性。UPGMA聚类分析揭示了花椰菜自交系的熟期与其遗传差异相关。     

Genetic analysis of ORF5 of recent Korean porcine reproductive and respiratory syndrome viruses (PRRSVs) in viremic sera collected from MLV-vaccinating or non-vaccinating farms

The 23 open reading frame (ORF) 5 sequences of Korean type II porcine reproductive and respiratory syndrome virus (PRRSV) were collected from viremic sera from the (modified live vaccine) MLV-vaccinating and non-vaccinating farms from 2007 to 2008. The samples were phylogenetically analyzed with previous ORF5 sequences, including type I Korean PRRSV, and previously reported or collected sequences from 1997 to 2008. A MN184-like subgroup of type II Korean PRRSV was newly identified in the viremic sera collected from 2007 to 2008. And of the type I PRRSVs, one subgroup had 87.2~88.9% similarity with the Lelystad virus, showing a close relationship with the 27~2003 strain of Spain. The maximum parsimony tree of type II PRRSV from 1997 to 2008 showed that they had evolved to four lineages, subgroups 1, 2, 3 and 4. Most of the recently collected type II PRRSVs belonged to subgroup 4 (48%). The region of three B-cell epitopes and two T-cell epitopes of ORF5 amino acids sequences was considerably different from the MLV in subgroups 3 and 4. In conclusion, the existence of type I PRRSV, which was genetically different from Lelystad virus (Prototype of type I PRRSV), and heterologous type II PRRSVs of viremic pigs detected even in the MLV-vaccinating farms indicated the need for new vaccine approaches for the control of PRRSV in Korea.     

杂交水稻施用粉煤灰磁化复合肥肥效研究

为检验粉煤灰磁化复合肥对杂交水稻的肥效 ,我们于 1998～ 1999年在信阳农专试验农场进行了杂交水稻施用粉煤灰磁化复合肥的试验研究 ,结果表明 :粉煤灰磁化复合肥在改善水稻生物学及经济性状 ,提高产量方面有显著的效应     

姑婆山国家森林公园景观格局变化分析

根据姑婆山国家森林公园1999年和2009年森林调查资料,在ArcGIS和Fragstas 3.3的支持下对姑婆山国家森林公园景观格局动态变化进行研究。结果表明：研究期间,研究区主要景观类型没有变化,阔叶树、灌木林、杉木林3种主要景观类型面积占总面积的比例由80.44%提高到94.51%;景观基质均为阔叶林,占总面积比例由63.63%提高到75.82%;总斑块数量由301块下降到174块;平均形状指数呈下降趋势;多样指数由1.233下降到0.862;优势度指数则由0.846升高至1.217。总体表现为针叶林向针阔混交林,进而向阔叶林演替,景观趋向连续和稳定。     

Effects of dietary protein to carbohydrate ratios on growth and body composition of juvenile yellow catfish, Pelteobagrus fulvidraco (Siluriformes, Bagridae, Pelteobagrus)

The present experiment was conducted to investigate the effects of dietary protein to carbohydrate ratios on growth and body composition of juvenile yellow catfish, Pelteobagrus fulvidraco . Nine diets were formulated to contain three protein levels (30%, 36% and 42%), each with three carbohydrate levels (24%, 30% and 36%). Each diet was randomly assigned to triplicate groups of 20 fish (initial mean body weight: 8.24±0.20 g) in indoor flow – through fibreglass tanks. The experiment continued for 8 weeks. Weight gain and specific growth rate were similar for the fish fed the 36% and 42% protein diets but higher than that fed the 30% protein diet. At the 36% protein level, carbohydrate contents varying from 24% to 36% ( P / E ratio of 24.0–28.2 mg protein kJ⁻¹) had no significant effects on growth performance and feed utilization ( P >0.05). Protein efficiency ratio tended to increase with dietary carbohydrate level at the same protein level. Dietary treatments significantly influenced body composition ( P <0.05), but not the condition factor, viscerosomatic index, hepatosomatic index and intraperitoneal fat ratio ( P >0.05). Based on these observations, 36% protein and 24–36% carbohydrate with the P / E ratio of 24.0–28.2 mg protein kJ⁻¹ seemed suitable for optimal growth and feed utilization, and carbohydrate could cause protein-sparing effect in diets for juvenile yellow catfish.     

高压气动比例减压阀设计与仿真

提出一种高压气动比例减压阀,该阀由比例电磁铁控制的二位三通型滑阀式先导阀和活塞提升式主阀组成,通过压力传感器和控制器构成闭环电反馈控制,最高工作压力为31.5 MPa。该阀虽然存在少量先导耗气,但保证了压力调整的快速性和稳定性,克服了先导泄漏对减压阀的影响,避免了先导阀意外结冰的发生。在介绍结构及工作原理的基础上分析了该减压阀的特点,利用AMESim建立了考虑气源压力和负载流量波动的仿真模型。仿真结果表明:该阀在气源压力缓慢下降且负载流量大范围波动的情况下能实现稳定的压力输出,在不改变控制参数的前提下,当气源压力为31.5 MPa时输出压力可在1~30 MPa范围内稳定;先导阀预开口形式对压力精度影响较小,但应避免正开口以减少气体浪费;先导阀环形间隙高度控制在10μm左右较为合适。     

不同地面结构育肥猪舍的恶臭排放影响因素分析

研究了冷、暖季节下生物发酵床、水泥实心、全缝隙3种不同地面结构的机械通风育肥猪舍的恶臭浓度、排放系数及地面结构、舍内温湿度、猪体重对恶臭浓度的影响.研究表明:猪舍的恶臭浓度与舍内温度、猪体重呈显著正相关(p＜0.05),恶臭浓度与相对湿度无显著相关性(p＞0.1);地面结构不同,恶臭浓度存在显著差异(p＜0.05);生物发酵床猪舍有助于恶臭减排,恶臭浓度最低,水泥实心地面猪舍的恶臭浓度最高,全缝隙地面猪舍的恶臭浓度居中;生物发酵床、全缝隙、水泥实心地面育肥猪舍的恶臭排放系数分别为(3.39±3.33)、(3.70±1.31)、(4.33±2.39) OU/(m2·s).     

鸡粪厌氧发酵沼液混凝处理投药量预测

对某养鸡场沼气工程全混式发酵罐出水沼液混凝投药量预测模型进行了研究.采用回归分析法确定了混凝剂最优投加量的模型参数;通过回归正交试验研究了助凝剂最优投加量影响因素,建立了助凝剂最优投加量预测模型;通过中试试验,对所得投药量模型进行了验证.结果表明,混凝处理沼液混凝剂最优投加量模型为A=0.000 06C1.41;助凝剂最优投加量模型为B=0.000 148CN -0.014 2C -0.277N+ 26.7,原水CODcr及搅拌强度是影响助凝剂PAM投加量的重要因素;经统计检验及中试试验验证,表明所建预测模型准确可靠.     

浅析新时期我国农业推广中存在的问题及科学应对策略

依据我国新时期农业发展的优势环境展开了农业推广进程中普遍存在问题的深入分析,并针对目前农业发展中重科研、轻推广,机制建立不健全等现状制定了切实可行的应对策略,对促进我国农业的成果转化与合理推广有重要的实践意义。     

Dissecting Genetic Basis of Deep Rooting in Dongxiang Wild Rice

Deep rooting is an important trait in rice drought resistance. Genetic resources of deep-rooting varieties are valuable in breeding of water-saving and drought-resistant rice. In the present study, 234 BC₂F₇ backcross introgression lines were derived from a cross of Dongye 80 (an accession of Dongxiang wild rice as the donor parent) and R974 (an indica restorer line as the recurrent parent). A genetic linkage map containing 1 977 bin markers was constructed by ddRADSeq for QTL analysis. Thirty-one QTLs for four root traits (the number of deep roots, the number of shallow roots, the total number of deep roots and the ratio of deep roots) were assessed on six rice chromosomes in two environments (2020 Shanghai and 2021 Hainan). Two of the QTLs, qDR5.1 and qTR5.2, were located on chromosome 5 in a 70-kb interval. They were detected in both environments. qDR5.1 explained 13.35% of the phenotypic variance in 2020 Shanghai and 12.01% of the phenotypic variance in 2021 Hainan. qTR5.2 accounted for 10.88% and 10.93% of the phenotypic variance, respectively. One QTL (qRDR2.2) for the ratio of deep roots was detected on chromosome 2 in a 210-kb interval and accounted for 6.72% of the phenotypic variance in 2020. The positive effects of these three QTLs were all from Dongxiang wild rice. Furthermore, nine and four putative candidate genes were identified in qRDR2.2 and qDR5.1/qTR5.2, respectively. These findings added to our knowledge of the genetic control of root traits in rice. In addition, this study will facilitate the future isolation of candidate genes of the deep-rooting trait and the utilization of Dongxiang wild rice in the improvement of rice drought resistance.