Genome Array on Differentially Expressed Genes of Skin Tissue in Cashmere Goat at Early Anagen of Cashmere Growth Cycle Using DNA Microarray

In order to study the molecular mechanism involved in cashmere regeneration, this study investigated the gene expression profile of skin tissue at various stages of the cashmere growth cycle and screen differentially expressed genes at proangen in 10 cashmere goats at 2 years of age using agilent sheep oligo microarray. Significance analysis of microarray （SAM） methods was used to identify the differentially expressed genes, Hierarchical clustering was performed to clarify these genes in association with different cashmere growth stages, and GO （Gene ontology） and the pathway analyses were con-ducted by a free web-based Molecular Annotation System3.0 （MAS 3.0）. Approximately 10200 probe sets were detected in skin tissue of 2-yr-old cashmere goat. After SAM analysis of the microarray data, totally 417 genes were shown to be differentially expressed at different cashmere growth stages, and 24 genes are significantly up-regulated （21） or down-regulated （3） at proangen concurrently compared to angen and telogen. Hierarchical clustering analysis clearly distinguished the differentially expressed genes of each stage. GO analysis indicated that these altered genes at proangen were predominantly involved in collagen fibril organization, integrin-mediated signaling pathway, cell-matrix adhesion, cell adhesion, transforming growth factor-β （TGF-β） receptor signaling pathway, regulation of cell growth. Kyoto encyclopedia of genes and genomes （KEGG） analysis showed that the significant pathways involved mainly included focal adhesion and extracellular matrixc （ECM）-receptor interaction. Some important genes involved in these biological processes, such as COL1A1, COL1A2, COL3A1, SPARC, CYR61 and CTGF, were related to tissue remolding and repairing and detected by more than one probe with similar expression trends at different stages of cashmere growth cycle. The different expression of these genes may contribute to understanding the molecular mechanism of cashmere regeneration.     

新高系砂梨品种引种栽培试验研究初报

为了筛选适于我国长江中游地区栽培的砂梨优良品种,以引种至湖南株洲的9个新高系砂梨品种为对象,就其生长特性、物候期、果实品质、丰产性、抗病性等方面的特性进行了为期5年的比较观察与试验,结果表明:该9个品种中华丰、华高、天皇这3个品种的表现最好,可在湖南及其周边地区大力发展,新高、黄金这2个品种的表现良好,可适当发展。     

野扁桃ISSR-PCR反应体系的优化

以野扁桃为材料,对影响ISSR-PCR扩增结果的Mg2 、dNTPs、Taq酶、模板DNA、引物等因素进行筛选和优化,确立了适合野扁桃ISSR分析的最佳反应体系:25μL PCR反应体系中包含1×Buffer,2.0 mmol.L-1Mg2 ,160μmol.L-1dNTPs,模板DNA30 ng,Taq聚合酶1 U,引物15 pmol。     

氮磷亏缺条件下杨树幼苗根系分泌酚酸的动态

采用循环收集装置收集杨树幼苗根系分泌物,采用超高压液相色谱法(UPLC)对根系分泌物中的酚酸含量进行测定。结果表明:在正常、缺N、缺P处理下杨树幼苗根系均能分泌对羟基苯甲酸、香草醛、苯甲酸和肉桂酸等酚酸类物质,养分缺乏显著促进杨树根系分泌酚酸。在缺N条件下,杨树幼苗较正常培养条件下酚酸分泌增加量为对羟基苯甲酸125%～263%、香草醛201%～316%、苯甲酸230%～332%、肉桂酸138%～188%,且根系分泌的各酚酸量随着胁迫时间的延长呈明显增加趋势,但3个培养时间段内(0～5天,6～10天,11～15天)酚酸分泌量差异未达显著水平(P>0.05);在缺P条件下,杨树幼苗较正常培养条件下酚酸分泌增加量为:对羟基苯甲酸96%～124%、香草醛58%～156%、苯甲酸222%～734%、肉桂酸99%～328%,其中,苯甲酸和香草醛的分泌量随胁迫时间的延长呈明显增加趋势,而苯甲酸和肉桂酸呈现先升后降的趋势。缺N、缺P处理下,杨树幼苗根系分泌酚酸的平均速率也存在一定差异,对羟基苯甲酸和香草醛在缺N条件下的平均分泌速率高于缺P培养,而苯甲酸和肉桂酸的平均分泌速率低于缺P培养。杨树幼苗在N,P缺乏条件下根系分泌酚酸...     

早熟、高产转基因抗虫常规棉品种——湘K25

概述了湘K25的选育经过,介绍了湘K25的特征特性、产量、纤维品质和抗病、抗虫等特点,并总结了其关键栽培技术。     

论玉兰属树种及其在园林绿化上的应用

玉兰属树种是中国传统观赏花木和药用及香料植物.依据相关文献,对玉兰属的主要树种和品种资源及其生物学特性、分布、主要观赏特性和用途进行了归纳评价,挖掘总结了其丰富的文史内涵,系统阐述了玉兰属树种在园林绿化中的应用途径.     

锦屏县毛竹现状分析与产业发展对策

毛竹产业的发展必须以市场为导向,以增加农民收入和促进山区农村经济发展为目标,创新发展方式,加大扶持力度,按照规模上求大、品种上求新、管理上求精的要求,坚持扩大面积与提高种植质量并重,强化科技支持与产业经营体系建设,把毛竹子产业培育成山区经济发展的重要支柱产业。     

Soybean plant height QTL mapping and meta‐analysis for mining candidate genes

Plant height is closely related to seed yield of soybean. The goal of this study was to identify important loci affecting soybean plant height using meta‐analysis based on a reference physical map. Plant height related to QTLs was mapped across eight years with a RIL population by WinQTLCart v2.5. 182 QTLs related to plant height of soybean from database and our research were collected, and each QTL was projected onto the soybean physical map by software BioMercator v2.1. The confidence interval of meta‐QTL ranged from 0.09 to 5.07 Mb, and the mean phenotypic variance ranged from 4.9% to 73.0%. Furthermore, 4,259 candidate genes were located in these consensus QTLs, and 40 of them were involved in the plant growth and stem elongation and annotated as plant hormone signal transduction (pathway ID ko04075) in KEGG pathway. These results would lay a foundation for fine mapping of QTLs/genes related to plant height and marker‐assisted selection for breeding in soybean.     

N2O production in the organic and mineral horizons of soil had different responses to increasing temperature

Journal of Soils and Sediments - The responses of N2O emission to increasing temperature in different soil horizons are not clearly understood yet. Here, we investigated the effects of increasing...