鸭DCN基因编码区扩增、序列分析及其在大肠杆菌中的表达、蛋白纯化

采用RT-PCR方法从鸭腿肌总RNA中扩增了鸭核心蛋白聚糖基因编码区序列,进行序列分析,并将编码鸭核心蛋白聚糖成熟蛋白的核酸片段连入原核表达载体pET-32a(+)中,加入IPTG诱导其表达后,亲和层析纯化表达的蛋白。采用SDS-PAGE检测,用质谱方法对表达的蛋白进行鉴定。研究成功克隆出鸭核心蛋白聚糖基因编码区序列,序列分析表明,鸭DCN编码区序列由1074个碱基组成,编码357个氨基酸,其中信号肽有16个氨基酸。将鸭DCN蛋白划分为9个结构域(LRR1-9),结合人DCN序列进行序列分析推测结构域中LRR4、5与TGF-β可能存在一定关系。SDS-PAGE检测和质谱鉴定结果表明获得了分子量为54.7ku的鸭DCN融合蛋白。     

Effects of active immunization against myostatin on carcass quality and expression of the myostatin gene in pigs

The study was conducted to investigate the effects of active immunization against myostatin on the titer of myostatin antibody, carcass evaluation, activity of creatine kinase and the expression of the myostatin gene in pigs. Eighteen pigs were allotted into three groups (six pigs per group), and pigs in treatment 1, 2 and 3 were immunized with physiological saline, 1 mg or 4 mg myostatin per pig, respectively. Six pigs were killed by electrical stunning followed by exsanguination at BW of 100 kg. The results indicated that the titer of myostatin antibody was increased in treated groups compared to the control group on day 42 ( P  < 0.01) and d 84 ( P  < 0.01). The carcass lean percentage was significantly increased in the treatment groups compared to the control group ( P  < 0.01), and intramuscular fat was significantly decreased in the 4 mg group compared to the control group ( P  < 0.05). The muscle creatine kinase activity of pigs treated with 1 mg and 4 mg myostatin was lower than the control group. The immunization of myostatin signofocantly decreased the myostatin gene expression levels in muscle. It was concluded that optimal active immunization against myostatin could increase the content of myostatin antibody, suppress the activity of creatine kinase and the expression of myostatin gene, and therefore improve the carcass lean percentage for pigs.     

巴音布鲁克羊生长规律的研究

选择0～6月龄的纯种巴音布鲁克羊羔羊和巴音布鲁克羊经济杂交羔羊,在自然放牧状态下,观察羔羊生长规律及经济杂交效果。通过对母羊妊娠后期进行补饲,研究补饲对羔羊产后生长的影响。结果表明,巴音布鲁克羊从出生到6月龄的过程中,2～3月龄增长最快,其次是1～2月龄、0～1月龄;4～5月龄体重下降,5～6月龄又开始增加。妊娠后期母羊补饲对羔羊产后生长没有明显影响,经济杂交羔羊杂交优势明显,公、母羔羊累计生长体重均极显著高于纯种巴音布鲁克羔羊。所以根据羔羊生长规律,加强羔羊生长高峰期营养和管理,是提高巴音布鲁克羊经济效益的重要措施。     

贵州白香猪的遗传检测

选取27个微卫星位点,应用PCR技术对贵州白香猪的2个品系进行遗传质量监测。结果在所选的27个微卫星位点中,共有24个位点获得稳定的扩增结果,其中SW911位点与S0026两个位点在群体内表现为单态纯合。2个品系有效等位基因、平均多态信息含量、平均杂合度、基因纯合率和平均近交系数分别为1.8310、1.7951、0.3436、0.3249、0.4329、0.4188、52.63%、58.55%、0.5377、0.5605。结果表明微卫星标记可用于检测贵州白香猪的遗传质量,也可用于分析封闭群的遗传多态性。是一种有潜力的试验动物遗传学检测标记。     

16个紫花苜蓿品种在通辽地区气候适宜性试验研究

为促进紫花苜蓿产业健康发展,该研究对引种的16个紫花苜蓿品种在内蒙古自治区通辽市舍伯吐镇进行气候适宜性试验,对各苜蓿品种的生产表现、产量和营养品质进行研究,并采用隶属函数法对各品种苜蓿的生产性能和营养品质进行综合评价.结果表明,16个紫花苜蓿品种中旱地、公农1号、金皇后、敖汉综合性能表现较好,适宜在通辽地区推广.     

藏獒皮肤成纤维细胞培养与生物学特性观察

In order to study biological characteristics of Tibetan Mastiff somatic cells and preserve genetic resource, two fibroblast cell strains from Tibetan Mastiff were established by tissue culture. In vitro culture cell morphology, hyperplasia ability and some biological characteristics were researched. Tibetan Mastiff cells in vitro culture appeared typical morphology of fibroblast cells, grew well and had strong hyperplasia ability. Population doubling time of the cells was 46.1 h. Vimentin expression in the cells was confirmed by immunofluorescence staining. The rate of F5 cells with normal diploidy (2n=78) was 91.4%. Moreover, longer X chromosome belonged to submetacentric chromosome, but Y chromosome did telocentric chromosome. Tibetan Mastiff cell strains eslablished in the study offered the materials for the Tibetan Mastiff somatic cell nuclear transfer research.     

相同养殖模式下,大黄鱼不同投喂模式的效益对比

<正>1福建宁德大黄鱼养殖背景福建宁德海区由于天然环境优异,经过多年的养殖,现已成为国内大黄鱼养殖最集中的地方。在人工养殖大黄鱼的过程中,多年来凭借海区水域内天然饵料丰富的优势,以低廉的活饵料养殖成为目前海区主要的投喂模式,养户以活饵料成本低和成     

紫花苜蓿草渣及浆汁发酵研究

为有效利用紫花苜蓿Medicago sativa深加工工业所得的浆汁和草渣,对紫花苜蓿浆汁和草渣的发酵进行了初步研究。研究发现苜蓿浆汁发酵较草渣发酵更容易。适当添加葡萄糖和发酵汁可以促进发酵,但添加葡萄糖质量分数超过2%后,促进作用并不明显,添加浆汁和草渣发酵的葡萄糖的最适宜质量分数分别为1%和2%。适当提高发酵温度可以加速发酵,过高和过低的温度均不利于发酵的顺利进行,研究中最适宜的发酵温度为35℃。在试验所选取的3个不同发育时期(分枝期、开花期和结荚期)的发酵原料中,结荚期苜蓿的发酵效果最好。     

牛磺酸的生物学功能及其在蛋鸡生产中的研究应用

牛磺酸是一种具有广泛生物学功能的营养物质,它在畜牧业生产中的应用已引起人们的密切关注,成为当今研究的一个亮点。本文概述了牛磺酸的结构、性质、分布、合成代谢、生物学功能及其在蛋鸡生产中的研究应用进展,并指出了今后需解决的问题和发展前景。     

不同发育期牦牛红细胞数、血红蛋白及肌红蛋白的测定及意义

用常规方法对同一海拔高度不同发育期牦牛几项指标进行测定。结果:随着牦牛年龄的增长血液中Hb含量、HCT值逐渐增加,而RBC明显降低,差异显著(P<0.05),即随年龄的增长Hb、HCT与RBC的变化呈负相关;心肌和骨骼肌Mb含量随着年龄的增长均呈逐渐递增的趋势,心肌中Mb含量均显著高于骨骼肌,差异显著(P<0.05)。结论:牦牛在生长发育过程中逐渐增加血液中Hb、HCT和骨骼肌、心肌中Mb含量增强机体有氧代谢机制。