Expression of IL-6 mRNA in endometrium with endometriosis

AIM: The purpose of the present study was to explore the relationship between interleukin-6 mRNA expression and endometriosis. METHODS: Using the rat model, IL-6 mRNA expression in the endometrium was examined by RT-PCR. RESULTS: The expression of IL-6 mRNA in control rats did not change at 2, 4, 6 and 8 weeks after sham operation (P>0.05), but in model rats it gradually increased at 2, 4, 6 and 8 weeks after endometriosis (P<0.01). The expression of IL-6 mRNA in uterine endometrium with endometriosis was lower than in endometriotic tissue, but higher than in endometrium from healthy controls. CONCLUSION: The IL-6 mRNA expression may contribute to the development of endometriosis . The increase in IL-6 mRNA expression may promote the implantation and growth of endometriotic tissue.     

Effects of platelet-derived growth factor on DNA and collagen protein synthesis in human vascular fibroblasts

AIM: To investigate the effects of platelet-derived growth factor on DNA and collagen protein synthesis in human vascular fibroblasts. METHODS: In the present experiment, the human vascular fibroblasts were cultured and effects of platelet-derived growth factor-BB on DNA and collagen protein synthesis in human vascular fibroblasts were observed by using [³H]-TdR incorporation and [³H]-proline incorporation in vitro. RESULTS: Platelet-derived growth factor-BB significantly promoted NDA synthesis and collagen protein synthesis of quiescent human vascular fibroblasts, with a maximal response at a concentration of 30μg·L^-1at 24 h and 36 h, respectively. CONCLUSION: Platelet-derived growth factor-BB promotes DNA and collagen protein synthesis in cultured human vascular fibroblasts.     

Protein expression of cyclin D2 and p16 in proliferation and differentiation of cultured cardiac myocytes

AIM:To investigate the protein expression of cyclin D₂ and p16 in proliferation and differentiation of cultured cardiac myocytes.METHODS:One-day-old Sparague-Dawley rats were used. Cardiac myocytes(CM) were collected by a trypsin-dispersal method and cultured. Cell growth line and fluorescence activated cell sorting (FACS) were used to investigate the proliferation of CM. Ultra-thin sections were made to observe the ultrastructure of CM under transmission electron microscope. The expression of cyclin D₂ and p16 in CM were measured using immunocytochemistry and image analysis.RESULTS:①Results of cell growth line and FACS analysis showed that cultured CM could proliferate in the first 3 cultured days, but the ability decreased quickly, concomitant with differentiation. CM was obseved quiescent in cell cycle three days later. The ultrastructure of CM showed the large amount of myofilaments and mitochondrion. ②The protein expression of cyclin D₂ in 3,4,5 day CM group was 0.89 times(P＜0.05),0.80 times (P＜0.05) and 0.56 times (P＜0.01) of that in 1 day group, respectively. The expression of p16 in CM was increased during the culture process, 2,3,4,5 day group were 1.63 times, 1.72 times, 1.99 times and 2.84 times (P＜0.01) of that in 1 day group, respectively.CONCLUSION:Cultured neonatal rat cardiac myocytes could proliferate during the first 3 days after incubation, but the ability of proliferation decreased, from the fourth day, concomitant with differentiation. Cyclin D₂ and p16 play the key roles in CM postnatal development. Downregulation of cyclin D₂ and upregulation of p16 may induce CM differentiation.     

钻蛀板栗枝干害虫的发生情况分析

本文对钻蛀板栗枝干害虫的发生分布、致树木死亡的发生量和不同树龄期的发生差异等情况进行了分析。从中找到了一些规律，可供有关方面应用。     

螟虫长距茧蜂研究进展

本文综述了对螟虫长距茧蜂的研究概况，包括该寄生蜂的主要生物学习性、对玉米螟自然寄生率及田间防治玉米螟效果等。     

棉疫病菌elicitin基因的克隆与序列分析

 棉疫病菌是我国棉花和苎麻疫病的主要病原菌,引起棉花烂铃、死苗,苎麻叶斑和茎腐,严重影响棉花和苎麻的产量和品质^[1,2],该菌的主要寄主有棉花、苎麻和构树^[3]。     

甘肃东方蜜蜂的形态特征研究

通过测定甘肃2个不同样点的4群东方蜜蜂发现,甘肃蜜蜂种群内遗传变异丰富,种群中许多形态性状特征表现出较明显的地理变异性;蜜蜂个体增大、体色变深,具体是:前翅长、前翅宽、背板3,4宽;后腿长、蜡镜长、蜡镜宽、蜡镜间距、腹片3,6的长和宽逐渐增加;背板3,4、小盾片的色素度逐渐加深。这些均具有生态适应意义。     

检测猪流行性腹泻病毒的R-PCR方法的建立

根据猪流行性腹泻病毒 (PEDV)的N基因自行设计和合成了一对可扩增长度为 641bp目的片段的引物 ,成功地建立了检测的猪流行性腹泻病毒的RT PCR方法。对猪轮状病毒 (PRV)、猪传染性胃肠炎病毒 (TGEV)的RT PCR检测结果均呈阴性。对PEDV JS株的RT PCR产物的序列分析表明 ,与CV777株的同源性为 97 3 %。     

差异显示法研究不同锌源对组织基因表达的影响

选用Avine肉用仔鸡作为试验对象 ,饲喂硫酸锌和蛋氨酸锌 2种锌源。采用银染差异显示技术进行差异基因的初步筛选。结果成功回收扩增差异条带 2 6条 ;其中加锌组 (硫酸锌组和蛋氨酸锌组 ) 2 3条 ,3条为缺锌组所特有。在加锌组的 2 3条中 ,6条为蛋氨酸锌组所特有 ,1 3条为硫酸锌组所特有 ,其余 4条为 2组所共有。这些初步筛选得到的差异基因尚需进一步进行克隆、测序和杂交进行验证。     

利用废菌糠提高覆土持水力和蘑菇产量的研究

本文利用平菇等食用菌的废菌糠 ,经适当无氧发酵等一系列处理后添加到普通泥土中 ,使其最大持水力提高至 5 1 3% ,单产提高 13 6 %