芋优异种质资源鉴定评价研究

对农业行业标准《农作物种质资源鉴定评价规范芋》编制的技术路线进行了分析研究,以期为芋优异种质资源的鉴定评价提供参考。     

过量磷对菜心植株生长和产量及品质的影响

采用营养液水培法,研究过量磷对菜心(Brassica parachinensis)植株生长、产量和品质的影响.结果表明:与正常磷浓度处理(1 mmol/L H2PO4-)比较,3～7mmol/L H2P04-处理植株生物量和产量均显著降低,5和7 mmol/L H2PO4-处理植株根冠比显著增加;随着磷浓度的增加,菜薹表皮花色苷含量和菜薹可溶性糖含量逐渐增加,菜薹硝酸盐含量逐渐降低,三者与营养液磷浓度均呈显著线性关系.     

无子沙糖橘(十月橘)的选育

采用芽变选种的方法,在四会市石狗镇选出了无子柑橘新品种—无子沙糖橘(十月橘)。果略小,单果重平均40g左右,单果平均种子数0.5粒以内,达到柑橘无子的标准。果实扁平,皮橘红色,薄而脆;果肉橙黄色,味清甜,可溶性固形物13%以上,肉质爽脆化渣,品质上等。早结丰产性好,发梢能力强,3月上中旬开花,12月上旬果实开始成熟。     

多菌灵对茶树菇菌丝生长的影响及其在子实体中的残留

研究了多菌灵对茶树菇和食用菌生产中3大主要污染霉菌菌丝生长的影响.在PDA培养基上多菌灵对茶树菇菌丝和孢菌、木霉及曲霉菌丝的EC50分别为22.3μg·mL-1、0.06μg·mL-1、0.29μg·mL-1和0.64μg·mL-1,MIC分别为60μg·mL-1、0.9μg·mL-1、1.6μg·mL-1和2.0μg·mL-1;在出菇袋料培养基中,按1500、11000和12000添加多菌灵对茶树菇菌丝生长影响不明显.同时采用液相色谱法分析了多菌灵在茶树菇子实体中的残留,3种处理的残留量分别为0.09ppm、0.04ppm和0.01ppm.     

茶树新品种‘槎湾 3 号’

 ‘槎湾3 号’是从洞庭碧螺春茶树群体种中选育成的早生,优质,高产,抗逆性较强的碧螺春茶专用茶树新品种。属灌木型,小叶类,树姿半开展,节间短,茸毛较多。制绿茶品质优,尤其适制碧螺春,适宜苏南碧螺春茶主产区种植。     

Relationship between Th1/Th2 balance and CD28/CTLA-4 expression on peripheral blood T cells in patients with systemic lupus erythematosus

AIM: To investigate the pattern of Th1/Th2 balance in systemic lupus erythematosus(SLE) patients and the relationship between CD28/CTLA-4(cytotoxic T-lymphocyte antigen-4) molecule expression and Th1/Th2 balance.METHODS: Eighteen SLE patients met the ARA 1997 updated SLE criteria were selected in the study. According to Bombardier's SLEDAI criteria, all patients were classified into two groups: active group(12 cases) and static group(6 cases). Fourteen normal individuals, matched for age and sex of the patients, served as controls. The peripheral blood mononuclear cells(PBMCs) were isolated by density gradient centrifugation and cultured in RPMI-1640 culture medium. After treated with PMA(5 μg/L) and ionomycin(500 μg/L) for 72 h, the PBMCs were collected, the contents of IFN-γ and IL-10 in the supernatant of cultured PBMCs were detected using enzyme linked immunosorbent assay(ELISA). The expression of CD28 and CTLA-4 molecules on T cells were detected by flow cytometric technique with double staining by FITC or PE labeled monoclonal antibodies. RESULTS: The level of IL-10 was higher in the PBMCs of active and static SLE patients(351.29 ng/L±153.31 ng/L and 319.37 ng/L±153.39 ng/L) than that in controls(254.48 ng/L±120.69 ng/L), but the difference did not reach statistical significance(P>0.05). The level of IFN-γ was significantly lower in the PBMCs of active SLE patients(25.76 ng/L±16.09 ng/L) than that in controls(50.71 ng/L±27.92 ng/L, P＜0.05). The ratio of IL-10/IFN-γ was significantly higher in active SLE patients(18.74±13.77) than that in controls(6.66±4.95, P<0.05). Either before or after culture, the expression of CD28 molecule on CD3⁺and CD8⁺ T cells from all SLE patients was not remarkably different from that in the cells of controls. Before culture, the expression of CTLA-4 molecule on CD3⁺T cells of active SLE patients(0.79%+0.37%) was significantly lower than that in the cells of controls(1.31%+0.61%, P<0.05). After culture, the expression of CTLA-4 molecule on CD3⁺ T cells of SLE patients was still lower than that in the cells of normal controls without statistical significance(P＞0.05).The expression level of CD28 molecule on CD3⁺ or CD8⁺ T cells in active SLE patients and controls was not correlated with the levels of IFN-γ and IL-10 in the supernatants(P＞0.05). The level of CTLA-4 molecule expression on CD3⁺ T cells of active SLE patients was positively correlated with IFN-γ level(r=0.681, P<0.05), while was negatively correlated with IL-10 levels(r=-0.624,P＜0.05) and the ratio of IL-10/IFN-γ(r=-0.738, P<0.01). The level of CTLA-4 molecule expression on CD3⁺ or CD8⁺ T cells of controls showed no correlation with IFN-γ levels, while showed negative correlations with IL-10 level(r=-0.587, P<0.05; r=-0.563, P<0.05, respectively).CONCLUSION: There is a bias in the differentiation of Th0 cells towards Th2 in SLE patients. CTLA-4 probably plays an important role in this mechanism through suppressing the signal transmitted by CD28.     

无核椪柑新品种——丽椪2号的选育

丽椪2号无核椪柑是有核或部分果实无核椪柑的芽变单株?闷分治藓寺蚀?98%,并且果形端正、扁平、平均单果质量140g左右,可溶性固形物含量11%,总糖9.0%,含酸量0.9%,可食率68%,风味浓,丰产性和商品性好、抗逆性强,果实耐贮藏。适宜种植范围为浙江及全国类似地区的有核椪柑适栽区。     

不同处理对樱桃番茄贮藏特性的影响

用蔗糖基聚合物和施保克(咪鲜胺)对樱桃番茄进行采后处理.结果显示,施保克和蔗糖基聚合物处理能降低樱桃番茄的呼吸强度,抑制VC含量下降和过氧化物酶升高.另外,蔗糖基聚合物处理的果实SS,SPS的活性明显高于对照.高活性的SS和SPS有利于果实内糖分的积累.     

Increased expression of CD40 ligand by peripheral blood mononuclear cells in patients with systemic lupus erythematosus

AIM:To investigate expression and function of CD40 ligand by peripheral blood mononuclear cells (PBMCs) in patients with systemic lupus erythematosus (SLE).METHODS:Expression of CD40 ligand by PBMCs in patients with SLE and control were examined by flow cytometric analysis before and after stimulated by phytohemagglutinin(PHA)and depressed by Dexamethasone(Dex). The correlation between expression of CD40 ligand and SLE activity index(SLEDAI) was analysed in patients with SLE.RESULTS:The expression of CD40 ligand by PBMCs in patients with active SLE was higher than that in patients with inactive SLE and control. Though the expression of CD40 ligang by PBMCs could be stimulated by PHA in three groups, it was the highest in patients with active SLE. Dex depressed the expression of CD40 ligand by PBMCs significantly in patients with SLE, but not in control. There was high positive correlation between expression of CD40 ligand and SLEDAI in patients with active and inactive SLE.CONCLUSION:Increased expression of CD40L by PBMCs in patients with SLE may play an important role in pathogenesis of SLE.     

西部罗布麻的民族植物学研究

从民族植物学的观点介绍了罗布麻在我国西部各民族人民生活中的地位及利用情况,论述了罗布麻的利用价值、分类、地理分布、生物学特性及其研究历史和研究进展。