Determination of quinomethionate (6-methylquinoline-2,3-diyldithiocarbonate) residues in crops by in situ fluorometry.

A simple method is described for the quantitative determination of quinomethionate (6-methylquinoline- 2,3 - diyldithiocarbonate) in crops. The pesticide residue is extracted with acetonitrile and partitioned in petroleum ether. After separation from the co-extractives by thin layer chromatography (TLC), the fluorescence is measured directly on a silica gel TLC plate. An average of 89% recovery is obtained at the 0.05 ppm level in apples, peaches, pears, and tomatoes.     

试论和谐海事文化的建设

和谐文化是构建社会主义和谐社会的重要任务。海事管理的发展也将是迈入文化建设的必然趋势。作为交通行政执法机关的海事管理机构来说,构建和谐、健康、先进的海事文化,将促进水上安全监督管理水平的提高,促进服务经济社会发展的水平和能力的提高,促进海事事业的蓬勃发展。     

头功铁药散对A型魏氏梭菌α毒素的mRNA表达及损伤小鼠免疫功能的影响

探讨自拟处方头功铁药散对A型魏氏梭菌α-毒素损伤小鼠免疫功能的影响,并检测了α毒素mRNA表达的情况.检测小鼠部分免疫功能;荧光定量RT-PCR法检测α毒素基因mRNA表达的变化.结果显示,与模型组相比,头功铁药散能拮抗α-毒素对小鼠的损伤,极显著提高小鼠免疫器官指数及巨噬细胞的吞噬能力(p0.01);与细菌模型组相比,头功铁药散能极显著降低α毒素基因表达(p0.01).推测头功铁药散可通过提高小鼠免疫功能,并下调α毒素基因表达,从而减少α-毒素对小鼠的损伤.     

High-Quality de novo Genome Assembly of Huajingxian 74, a Receptor Parent of Single Segment Substitution Lines

Rice(Oryza sativa L.)is grown nearly worldwide and provides the staple food for more than half of the global population(Luo et al,2017).The genomes of several cultivated rice varieties including Nipponbare(NPB)(Kawahara et al,2013;Sakai et al,2013),IR64(Tanaka et al,2020),93-11(Zhang et al,2018)and R498(Du et al,2017)at chromosome level,and Minghui 63 and Zhenshan 97(Zhang et al,2016)at scaffold level have been assembled,annotated and released,among which the R498 and NPB genomes are widely used as reference genomes in rice research.However,there are thousands of rice cultivars,landraces and wild rice varieties in the world with dramatically different genetic backgrounds,and the genomes of native rice varieties in South China,which is one of the major rice production areas in China,have not been de novo assembled.Huajingxian 74(HJX74)is an indica rice variety bred in South China Agricultural University,Guangdong Province with widely environmental adaptability and high yield(www.ricedata.cn/variety/varis/602548.htm).HJX74 exhibits significant phenotypic and genetic differences from those varieties whose whole genomes have been properly sequenced and assembled(Fig.1).     

Morphological Study of the Surface Structure of the Omasal Laminae in Cattle, Sheep and Goats

The mucosal relief of the omasum in cattle, sheep and goats was examined by the naked eye, stereoscopic microscope and scanning electron microscope. The mucosal relief of the omasum showed wide variations both between and within these species. Omasal papillae on the laminae varied highly in shape, i. e. conical, rounded, claw-like, wart-like, finger-like etc. They decreased gradually in size from the oral to the aboral region of the omasal lamina. Ridge-like structures and linear arrangements of the papillae were also seen on the omasal laminae of cattle and sheep. Many grooves and folds were found on the lateral surface of the omasal papillae in adult cattle and on the inter-papillar surface of the laminae of all three species. The mucosal relief of the omasum in cattle seemed to be more well-developed than those of sheep and goats. Dietary effects on the omasal mucosa were also discussed.     

Bronchoscopy in small animal medicine: indications, instrumentation, and techniques

Bronchoscopy is a useful tool in the evaluation and management of canine and feline respiratory diseases. Diagnostic indications include the evaluation of structural diseases (tracheobronchial collapse, stricture, intraluminal mass); inflammatory conditions (chronic bronchitis, pneumonia); and traumatic injuries. Several airway-sampling techniques are available with bronchoscopy; bronchoalveolar lavage has proved to be the most satisfactory specimen-collection technique. Therapeutic indications of bronchoscopy at this time in veterinary medicine are mainly limited to foreign body removal. As advances are made in veterinary bronchopulmonary medicine, other therapeutic applications of the bronchoscope may be realized.     

畜产品安全与毒害物质残留研究进展

论述了畜产品安全及其目前畜产品中的兽药、激素、重金属、生物毒素、农药、微生物等毒害物质的残留，并对畜产品安全现状及存在的问题进行了分析。     

Pharmacodynamic evaluation of a reference and a generic toltrazuril preparation in broilers experimentally infected with Eimeria tenella or E. acervulina

1. The aim of this study was to investigate the effects on pigmentation, faecal oocyst output, immune responsiveness and reactive oxygen species (ROS) generation following treatment with either the reference toltrazuril (Baycox) or a generic preparation (gen-TTZ), during an experimental Eimeria tenella (Et) or E. acervulina (Ea) infection of 210 Ross broiler chickens.

2. Results showed a significant difference on the anticoccidial efficacy 6 d after treating infected animals with Baycox (Et: 99.69% and Ea: 99.52%) or gen-TTZ (Et:85.71% and Ea 81.81%).

3. Gen-TTZ-treated animals were less strongly carotenoid-pigmented than Baycox-treated broilers. Mean plasma carotenoid concentrations were significantly higher in groups treated with Baycox than in broilers given gen-TTZ.

4. Treatment of animals with Baycox led to a significant decrease in ability of the peripheral blood mononuclear cells to produce ROS in contrast to gen-TTZ-treated groups. Baycox, but not generic toltrazuril, increased IL-10 and decreased tumour necrosis factor alpha (TNF-α) concentrations in chickens infected with E. tenella and E. acervulina.

5. It is suggested that differences in anticoccidial efficacy may be observed when using a generic toltrazuril product. Hence, in addition to plasma profiles of drugs, standardised clinical control tests may be necessary for generic formulations, particularly if other parameters are important to achieve a better control of coccidiosis.