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11.
A cat with acute myeloblastic leukemia without maturation (M1) treated with combination chemotherapy
Mashita T Shimoda T Yoshioka H Takahashi Y Mitsuda M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(1):97-101
A 2-year-old domestic shorthair cat was presented to us with decreased activity and anorexia. Hematologic findings revealed a mild non-regenerative anemia, thrombocytopenia, and leukocytosis with an increase in blast cells. Bone marrow aspirates also revealed a marked increase of blasts. The blastic cells were shown to be positive for peroxidase. Acute myeloblastic leukemia without maturation (M1) was diagnosed according to the FAB classification. Chemotherapy was initiated with cyclophosphamide, vincristine, prednisolone, and cytosine arabinoside. The cat responded partially. In total, the cats were given 7 blood transfusions. The cat died 14 weeks after first being presented to us. 相似文献
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以分离自西瓜上的Aac5菌株为例,通过同源重组的方法,构建了hrc N基因插入缺失突变体,通过PCR方法和Southern blot验证突变菌株,对突变体进行致病性、致敏性、生长曲线和运动性测定。为明确hrc N基因与其他基因的关系,通过实时荧光定量PCR法定量检测了hrp A、hrc V、hrc U、Lux I、LuxR 5个基因的表达量。结果显示:与野生型相比,突变体致病力和致敏性明显减弱,致病时间延迟,群体感应信号减弱,生长能力明显下降,运动性减弱,互补菌株只能恢复部分功能;5个基因在突变体中的表达量均上调,hrc N基因与这5个基因之间均为负调控关系。说明hrc N基因在果斑病菌致病能力上发挥重要作用。 相似文献
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为明确吉林省西瓜蔓枯病菌Stagonosporopsis citrulli对咪鲜胺和苯醚甲环唑的抗药性水平, 本试验采用菌丝生长速率法测定了151株西瓜蔓枯病菌对这两种药剂的敏感性及两种药剂对西瓜蔓枯病菌的MIC值, 并测定了吉林省7个西瓜主产区的375株菌株对这两种药剂的抗性水平。结果表明, 供试的151株菌株对这两种药剂的敏感性频率分布均呈连续的单峰曲线, 接近正态分布; 对咪鲜胺和苯醚甲环唑的敏感基线分别为(0.203 0±0.135 3) μg/mL和(0.675 9±0.394 2) μg/mL; 两种药剂的最低抑制浓度MIC分别为20 μg/mL和40 μg/mL。供试375株菌株对两种杀菌剂的抗性频率均为0。本研究结果为咪鲜胺和苯醚甲环唑的合理用药策略提供了科学依据, 为后续其抗性风险评估奠定了基础。 相似文献
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Ozaki K Nakahara Y Narama I 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(3):289-291
A dog presented with hematuria, and two small polypoid masses were detected in the urinary bladder. Histopathologically, the masses were located in the mucosal or submucosal layer. That tissue consisted of a random proliferation of spindle-shaped, round and pleomorphic cells with single or multiple large atypical nuclei and abundant cytoplasm, and eosinophil infiltration. These large cells were confirmed by immunohistochemical staining as fibroblasts, myofibroblasts and macrophages. Mitotic figure was rarely seen. These masses were diagnosed as eosinophilic polypoid cystitis with pseudosarcomatous proliferative tissue, since they consisted of a wide variety of cells and showed low growth activity. 相似文献
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Y.Hu B. Tan K. Mai Q.Ai S.Zheng K. Cheng 《饲料与畜牧》2009,(4):52-55
导读:虾料配制时首先考虑的因子。
前言
凡纳滨对虾原产于美洲中南部的太平洋沿岸,我国于1988年引进,由于该虾生长快、适应性强、食性广、价格高、抗病力强等优点,迅速成为主要养殖品种。当前,饲料成本占总养殖成本的50~60%,而蛋白水平又是影响生长性能和饲料成本的主要因素。那么怎样才能降低饲料成本呢?通过优化蛋白能量和非蛋白能量在饲料中的比例,可以达到这一目的。如果蛋白相对于能量过量,那么, 相似文献
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Maeda S Ohno K Nakamura K Uchida K Nakashima K Fukushima K Nakajima M Goto-Koshino Y Fujino Y Tsujimoto H 《Veterinary immunology and immunopathology》2012,148(3-4):226-235
The interaction between fractalkine/CX(3)CL1 and its receptor CX(3)CR1 has been reported to play an important role in various human inflammatory diseases, including inflammatory bowel disease (IBD) mediated by lymphocyte chemoattraction. The objective of this study was to investigate the role of fractalkine and CX(3)CR1 in lymphocyte migration in canine IBD. IBD was diagnosed in 34 dogs, and 19 healthy beagles were used as normal controls. We quantified intestinal mRNA and protein expression of fractalkine and CX(3)CR1 by real-time RT-PCR and ELISA, respectively, and examined the localization of fractalkine in canine intestine by immunohistochemistry. The expression of CX(3)CR1 and surface antigens on peripheral blood mononuclear cells (PBMCs) and intraepithelial lymphocytes (IELs) was analyzed by flow cytometry. Intestinal fractalkine and CX(3)CR1 mRNA was significantly up-regulated in IBD dogs compared with the healthy control dogs. In addition, fractalkine expression on intestinal epithelial cells was significantly increased in the intestinal mucosa of IBD dogs compared with the healthy dogs. CX(3)CR1(+) PBMCs were significantly elevated in IBD dogs and positively correlated with the histopathological severity of IELs infiltration. These CX(3)CR1(+) PBMCs predominantly expressed markers for cytotoxic T cells. Almost all IELs expressed CD3, and the majority of cells expressed CD8 rather than CD4, which was analogous to the CX(3)CR1(+) PBMCs. These results suggest that the fractalkine-CX(3)CR1 interaction may contribute to the pathogenesis of canine IBD through migration of IELs. 相似文献
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