Application of in vitro fertilisation techniques to obtain calves from valuable cows after slaughter.

The ovaries of two infertile cows of high breeding value were recovered after slaughter, and a total of 222 oocytes were obtained. Of these, 156 were classified as of good or fair quality and were subjected to in vitro maturation, in vitro fertilisation (using frozen semen from three bulls of high breeding value) and in vitro culture procedures. After eight days, 27 embryos were obtained, of which 13 were transferred fresh, and 14 were frozen. Three recipients of fresh embryos became pregnant; two calved and one aborted at four months. One of eight recipients of frozen-thawed embryos became pregnant but aborted at three months.     

氮硅添加对高寒草甸生物量和多样性的影响——以青藏高原为例

草地施肥多集中于添加氮肥与磷肥,很少涉及硅肥。硅作为对植物有益的一种元素,能提高植物对环境的抗性,促进植物的生长。本研究以青藏高原高寒草甸为研究对象,通过添加不同组合的氮肥和硅肥,研究群落地上生物量和生物多样性的变化。结果表明,氮肥和硅肥的添加均能提高群落的地上生物量,然而硅肥提高群落地上生物量的幅度远低于氮肥;在添加氮肥导致群落物种多样性下降的同时,添加硅肥可以缓解群落多样性下降的趋势;硅肥的生物学功能在群落水平上存在着最佳浓度效应。同时,我们推测硅肥在维持群落中杂草的存活率上发挥着积极的作用,并通过比较不同硅肥处理时,杂草生物量所占群落生物量比重的变化,支持了上述推测。     

Spatiotemporal heterogeneity of PPARγ expression in porcine uteroplacenta for regulating of placental angiogenesis through VEGF-mediated signalling

Non-infectious prenatal mortality severely affects the porcine industry, with pathological placentation as a likely key reason. Previous studies have demonstrated that peroxisome proliferator-activated receptor gamma (PPARγ) deficiency causes defects in the uteroplacental vasculature and induces embryonic losses in mice. However, its role in porcine placental angiogenesis remains unclear. In the present study, PPARγ expression was investigated in porcine uteroplacental tissues at gestational day (GD) 25, GD40 and GD70 via quantitative polymerase chain reaction (qPCR), Western blot and immunohistochemistry (IHC). Moreover, the roles of PPARγ in porcine placental angiogenesis were investigated using a cell model of porcine umbilical vein endothelial cells (PUVECs) to conduct proliferation, migration and tube formation assays in vitro and a mouse xenograft model to assess capillary formation in vivo. The results showed that PPARγ was mainly located in the glandular epithelium, trophoblast, amniotic chorion epithelium and vascular endothelium, as indicated by the higher expression levels at GD25 and GD40 than at GD70 in endometrium and by higher expression levels at GD40 and GD70 than at GD25 in placenta. Moreover, PPARγ expression was significantly downregulated in placenta with dead foetus. In PUVECs, knocking out PPARγ significantly inhibited proliferation, migration and tube formation in vitro and inhibited capillary formation in mouse xenografts in vivo by blocking S-phase, promoting apoptosis and downregulating the angiogenic factors of VEGF and its receptors. Overall, the spatiotemporal heterogeneity of PPARγ expression in porcine uteroplacental tissue suggests its vital role in endometrial remodelling and placental angiogenesis, and PPARγ regulates placental angiogenesis through VEGF-mediated signalling.     

犬外直耳道切除术的诊疗体会

犬外耳炎是指外耳道上皮的炎症。在临床上因其发病率高、病因多而复杂、治疗难度大、复发率高等原因,越来越受到关注。经久反复的外耳炎症导致的外耳溃疡、增生,以及外耳肿瘤等使犬的听觉减退和倍受疼痛与不适。犬的直耳道切除术对解决上述问题具有较好的治疗效果。本文对一例斗牛犬的直耳道炎性增生的直耳道切除术谈谈诊疗体会。     

抗菌素在犬病治疗过程中的合理应用

在各类细菌感染性犬病的治疗过程中,抗菌素是临床上最为广泛应用的药物之一,它在抗菌消炎、控制感染、促进康复方面发挥着重要作用.合理的抗菌素治疗,应根据犬病和个体的差异而选择不同抗菌素,从而达到有效的抑制或杀灭特异的病原体,并避免出现耐药性及药物副作用,使病犬治愈康复.     

陕西杨陵地区猪弓形虫病的血清学调查研究

应用间接血凝试验（ＩＨＡ）对采自杨陵区食品公司屠宰点、西北农业大学实验农场种猪场,西北农林科技大学兽医院的２１４份猪血样进行弓形虫抗体的定性检测,包括血清检测法（１７６份）,滤纸干血滴法（３８份）两种方法。结果检出阳性血样５份,阳性率为２．３４％。其中来自杨陵区食品公司屠宰点猪血样１３６份,全部用血清检测法测定结果,阳性５份,阳性率为３．６８％,来自西北农林科技大学农场种猪场血样７６份,３８份血清     

丙酸咪唑苯脲的研制及其对梨形虫病的治疗试验

以苯甲腈为起始原料 ,经硝化、环化、还原、缩合、成盐等五步反应合成抗梨形虫病新药丙酸咪唑苯脲 ,用 IR、UV、MS、1HNMR、13 CNMR、元素分析等对其化学结构进行了确证。UV法测定牛血药浓度及组织残留量。小白鼠 i P丙酸咪唑苯脲 ,LD50 =84.4± 1 3.5mg/kg。该药无致突变性和无致畸效应。用其治疗家畜梨形虫病 ,临床治愈率为 75%～ 1 0 0 %;预防水牛巴贝西虫病 ,试验组和对照组的发病率分别为 0 .72 %和 1 0 %,两组差异特别显著 ( P<0 .0 1 )     

饮高碘水对肉仔鸡生产性能及组织器官碘含量的影响

试验用艾维茵肉仔鸡１２０只,随机分为４组,分别饮用每千克含碘量为１５ｍｇ,２５ｍｇ,４５ｍｇ和０ｍｇ的饮水,饮用３７ｄ。结果表明：不同的碘水平对肉鸡生产性能、器官发育均未产生不良影响。但高碘饮水组鸡的组织碘含量均显著高于对照组鸡。     

一起猪球虫病感染诊断与防治措施

本文通过对湖南省岳阳市君山区某养猪场的10kg以下的发病仔猪进行临床症状观察和实验室诊断,确诊为猪球虫病。通过采用内服妥曲株利进行治疗,有效控制了病情。