Exploring the effectiveness of in ovo feeding of vitamin C based on the embryonic vitamin C synthesis and absorption in broiler chickensOA

Background: Many researches about in ovo feeding(IOF) of vitamin C(VC) are gradually carried out to explore physiological development in chicken, but little studies focus on VC synthesis capacity of the embryo itself, the selection of injection site and the effectiveness of IOF of VC. This study aims to explore the above problems.Results: Kidney and yolk sac were the main organs for VC synthesis and L-gulonolactone oxidase(GLO) expression was lower during pre-hatch development than that during p...     

浅谈克服东魁杨梅大小年的关键技术

近几年来,我镇果农大量种植杨梅,经统计全镇共种植320.6亩.由于品种较杂,存在产量低、品质差,平均每亩可增产223.9kg左右,个别年份虽产量高但品质差,增产不增收的现状.     

樱桃番茄主要农艺性状与产量的相关及通径分析

对9个樱桃番茄品种的8个农艺性状进行了相关和通径分析.相关分析结果表明,单株产量与每穗结果数呈极显著正相关,相关系数为0.5967.回归分析和通径分析结果表明,每穗结果数和单果重对单林产量形成的直接作用最大,单株穗数对产量的间接作用大;这三个性状是影响单株产量的主要因素,可作为樱桃番茄丰产育种的主要选择性状.     

Effects of exosomes derived from hypoxia-preconditioned umbilical cord mesenchymal stem cells on function of endothelial cells

AIM To investigate the effect of exosomes derived from hypoxia-preconditioned human umbilical cord mesenchymal stem cells (hUCMSCs) on proliferation, migration and tube formation of human umbilical vein endothelial cells (HUVECs). METHODS hUCMSCs and HUVECs were isolated, cultured and identified. Exosomes derived from hUCMSCs were extracted by ultracentrifugation. The morphological change of exosomes was observed under transmission electron microscope. The particle size and concentration of exosomes were detected by nanoparticle tracking analysis, and the surface specific marker proteins of exosomes were determined by Western blot. hUCMSCs were divided into normoxia group and hypoxia group. The viability of hUCMSCs was measured by CCK-8 assay. HUVECs were divided into control group, normoxic exosome group and hypoxic exosome group. The proliferation of HUVECs was detected by EdU assay. The migration ability was detected by cell scratch assay and Transwell experiment. Tube formation ability was evaluated by tube formation experiment. RESULTS Compared with normoxia group, hypoxia pretreatment enhanced the viability and exosome release of hUCMSCs. Compared with normoxic exosome group, hypoxic exosomes enhanced the proliferation, migration and tube formation of HUVECs. CONCLUSION Exosomes derived from hUCMSCs under hypoxia enhances the proliferation, migration and tube formation of HUVECs.     

Preparation and Characterization of Tilapia Collagen-Thermoplastic Polyurethane Composite Nanofiber Membranes

Marine collagen is an ideal material for tissue engineering due to its excellent biological properties. However, the limited mechanical properties and poor stability of marine collagen limit its application in tissue engineering. Here, collagen was extracted from the skin of tilapia (Oreochromis nilotica). Collagen-thermoplastic polyurethane (Col-TPU) fibrous membranes were prepared using tilapia collagen as a foundational material, and their physicochemical and biocompatibility were investigated. Fourier transform infrared spectroscopy results showed that thermoplastic polyurethane was successfully combined with collagen, and the triple helix structure of collagen was retained. X-ray diffraction and differential scanning calorimetry results showed relatively good compatibility between collagen and TPU.SEM results showed that the average diameter of the composite nanofiber membrane decreased with increasing thermoplastic polyurethane proportion. The mechanical evaluation and thermogravimetric analysis showed that the thermal stability and tensile properties of Col-TPU fibrous membranes were significantly improved with increasing TPU. Cytotoxicity experiments confirmed that fibrous membranes with different ratios of thermoplastic polyurethane content showed no significant toxicity to fibroblasts; Col-TPU fibrous membranes were conducive to the migration and adhesion of cells. Thus, these Col-TPU composite nanofiber membranes might be used as a potential biomaterial in tissue regeneration.     

苦荞遗传多样性分析与核心种质筛选

利用SSR分子标记技术,对来自西藏、陕西、四川、贵州等4省区的210份苦荞品种进行遗传多样性研究。结果表明,所选用的50对SSR引物中,有16对引物多态性良好,共扩增出178个条带,其中多态性条带达118个,占总数的66.3%;210份苦荞种质的遗传变幅为0.62~0.98,平均值为0.80,在遗传相似系数为0.88处可划分为5大类。聚类结果显示,来自同一地区的苦荞品种显示出聚为一类的趋势,表明苦荞的遗传信息受地理分布的影响较大;第V类所聚的41份分别来自西藏(27份)、陕西(8份)和贵州(6份)的品种表现出较为丰富的遗传多样性,与其他种质相比,这41份材料不仅遗传差异较大,而且遗传来源广泛,可作为苦荞核心种质筛选的基础。     

拮抗放线菌B1菌株鉴定及其防治番茄灰霉病的初步研究

为明确放线菌B1菌株在植物病害生物防治中的应用潜力,采用平板对峙培养法、离体叶片和果实及温室盆栽苗测定其抑菌作用,通过多相分类法研究其分类地位,并且采用单因子试验与正交试验相结合的方法,筛选出该菌株的液体发酵培养基配方.结果表明:菌株B1对10种植物病原真菌和3种植物病原细菌有抑制作用,其中对番茄灰霉病菌、番茄早疫病菌、小麦纹枯病菌、甘蓝黑腐病菌和西瓜果腐病菌的抑制作用较强,对番茄灰霉病菌的生长抑制率达86.25%;菌株B1的代谢产物对番茄灰霉病菌菌丝有致畸作用;菌株B1发酵上清液在离体叶片和活体植株上对番茄灰霉病具有良好的生防作用,其防治效果分别为68.80%和64.03%.根据形态特征、培养性状、生理生化特性和16S rDNA序列分析结果,将菌株B1初步鉴定为淡紫灰链霉菌Streptomyces lavendulae.通过正交试验筛选出菌株B1的液体发酵培养基组成为:葡萄糖15 g、玉米浆20g、NH4H2PO40.5 g、MgSO41.0 g、自来水1 000mL,pH 7.2～7.4.     

怎样避免新购幼犬应激反应综合征

新购幼犬易主后,由于突然间的改变地点、饮食、环境等因素,一周内易发生以消化系统和呼吸系统为主要症状的疾病,我们称为应激反应综合征。轻者易引起幼犬的身体发育不良或者其他疾病的并发症,重者可以导致幼犬的死亡。如何避免这种疾病?笔者认为首先应做好养犬前的准备工作。准备好犬的居住场所、饲喂用具和常用药品等。在购买时,一定要细心观察,切勿操之过急。为防止购入病犬,应注意幼犬的双眼眼睑无分泌物,眼球清洁,双眼有神,无上下眼睑内翻外翻等;双耳耳阔耳道内干净,无异物、异味;鼻镜湿润、冰凉,不干燥、不发热,嗅、视、听觉良好;皮肤…     

基于CRITIC-GR-TOPSIS法的烟台市水资源承载力综合评价

[目的]对山东省烟台市水资源承载具体状况进行定量与定性分析,提出相应提高水资源承载力的对策和建议,为实现烟台市水资源可持续发展提供科学参考.[方法]建立涵盖水资源、社会、经济和水环境4个准则层的评价指标体系,运用CRITIC-GR-TOPSIS法,对2003-2018年烟台市水资源承载力进行综合评价,以相对贴近度Cj作...     

HACCP在酸牛奶生产中的应用

本文主要探讨HACCP在酸奶生产中的应用，从质量卫生控制方面分析了生产过程中的危险因素，制定相应的控制方法和纠正措施，以取得高质量的酸牛奶。