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991.
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Litong Liu Xu Yang Pengfei Yuan Shanshan Cai Jing Bao Yanan Zhao Alimu Aimaier Adila Aipire Jun Lu Jinyao Li 《Marine drugs》2022,20(3)
Low molecular weight fucoidan (LMWF) has been reported to have immunomodulation effects through the increase of the activation and function of macrophages. In this study, the regulating effect of LMWF from Undaria pinnatifida grown in New Zealand on dendritic cells (DCs) was investigated. We discovered that LMWF could stimulate DCs’ maturation and migration, as well as CD4+ and CD8+ T cells’ proliferation in vitro. We proved that this immune promoting activity is activated through TLR4 and its downstream MAPK and NF–κB signaling pathways. Further in vivo (mouse model) investigation showed that LMWF has a strong immunological boosting effect, such as facilitating the proliferation of immune cells and increasing the index of immune organs. These findings suggest that LMWF has a positive immunomodulatory effect and is a promising candidate to supplement cancer immunotherapy. 相似文献
995.
Wei-Ping Ma Shi-Ning Yin Jia-Peng Chen Xi-Cheng Geng Ming-Fei Liu Hai-Hua Li Ming Liu Hong-Bing Liu 《Marine drugs》2022,20(3)
Hematopoietic damage is a serious side effect of cytotoxic drugs, and agents promoting hematopoiesis are quite important for decreasing the death rate in cancer patients. In our previous work, we prepared the simulated digestive product of fucoidan from Sargassum fusiforme, DSFF, and found that DSFF could activate macrophages. However, more investigations are needed to further evaluate whether DSFF could promote hematopoiesis in the chemotherapy process. In this study, the protective effect of DSFF (1.8–7.2 mg/kg, i.p.) on cyclophosphamide-induced hematopoietic damage in mice and the underlying mechanisms were investigated. Our results show that DSFF could restore the numbers of white blood cells, neutrophils, and platelets in the peripheral blood, and could also retard bone marrow cell decrease in mice with cyclophosphamide-induced hematopoietic damage. UPLC/Q-Extraction Orbitrap/MS/MS-based lipidomics results reveal 16 potential lipid biomarkers in a serum that responded to hematopoietic damage in mice. Among them, PC (20:1/14:0) and SM (18:0/22:0) were the key lipid molecules through which DSFF exerted protective actions. In a validation experiment, DSFF (6.25–100 μg/mL) could also promote K562 cell proliferation and differentiation in vitro. The current findings indicated that DSFF could affect the blood cells and bone marrow cells in vivo and thus showed good potential and application value in alleviating the hematopoietic damage caused by cyclophosphamide. 相似文献
996.
Wound healing is a highly orchestrated process involving many cell types, such as keratinocytes, fibroblasts and endothelial cells. This study aimed to evaluate the potential application of synthetic peptides derived from tilapia piscidin (TP)2, TP2-5 and TP2-6 in skin wound healing. The treatment of HaCaT keratinocytes with TP2-5 and TP2-6 did not cause cytotoxicity, but did enhance cell proliferation and migration, which could be attributed to the activation of epidermal growth factor receptor signaling. In CCD-966SK fibroblasts, although TP2-5 (31.25 μg/mL) and TP2-6 (125 μg/mL) showed cytotoxic effects, we observed the significant promotion of cell proliferation and migration at low concentrations. In addition, collagen I, collagen III, and keratinocyte growth factor were upregulated by the peptides. We further found that TP2-5 and TP2-6 showed pro-angiogenic properties, including the enhancement of human umbilical vein endothelial cell (HUVEC) migration and the promotion of neovascularization. In a murine model, wounds treated topically with TP2-5 and TP2-6 were reduced by day 2 post-injury and healed significantly faster than untreated wounds. Taken together, these findings demonstrate that both TP2-5 and TP2-6 have multifaceted effects when used as topical agents for accelerating wound healing. 相似文献
997.
果蔗间种花生可提高土地利用效率,提高果蔗种植整体经济效益,实现经济效益、社会效益、生态效益同步协调发展,促进广西果蔗产业可持续发展。为此,对果蔗间种花生的田地选择、整地、品种选择、播种与种植、田间管理、收获与留种、残膜回收的程序及其操作要求进行探讨,并提出规范和说明。 相似文献
998.
Effect of simulated transport stress on the rat small intestine: A morphological and gene expression study 总被引:2,自引:0,他引:2
Changrong Wan Peng Yin Xiaolong Xu Mingjiang Liu Shasha He Shixiu Song Fenghua Liu Jianqin Xu 《Research in veterinary science》2014
The present study investigated the effects of simulated transport stress on morphology and gene expression in the small intestine of laboratory rats. Sprague Dawley rats were subjected to 35 °C and 0.1×g on a constant temperature shaker for physiological, biochemical, morphological and microarray analysis before and after treatment. The treatment induced obvious stress responses with significant decreases in body weight (P < 0.01), increases in rectal temperature, serum corticosterone (CORT), serum glucose (GLU), creatine kinase (CK) and lactate dehydrogenase (LDH) levels (P < 0.01), as well as expression of Hsp27/70/90 mRNA (P < 0.05; P < 0.01). The rat jejunum was severely damaged and apoptotic after mimicking transport stress, which may mainly be related to cell death, oxidation reduction and hormone imbalance determined by microarray analysis. The bioinformatics analysis from the present study would provide insight into the potential mechanisms underlying transport stress-induced injury in the rat small intestine. 相似文献
999.
本研究选用72只6周龄京白鸡,随机分为A、B、C、D、E和F六组,分别饲喂钙、锌水平不同的日粮(各组锌、钙分别为:21.5ppm,0.95%;21.5ppm,1.95%;21.5ppm,2.95%;49ppm,0.95%;49ppm,1.95%;49ppm,2.95%),进行为期30天的饲养试验。试验中观察增重和临床变化;饲养结束时,取全血、羽毛、胫骨、肝和肾等组织的样本,测定其锌、钙、铁、铜、锰等元素的含量。试验结果是,D组增重和发育良好;A组稍次于D组;其他各组出现程度不同的临床症状,并且这些组所测组织锌含量明显低于D、A组,且其羽毛铁、骨铜、羽毛锰含量明显升高,而羽毛铜、肾铜及肾锰则显著降低。 相似文献
1000.
ZIKA种母兔饲粮适宜营养水平的研究 总被引:2,自引:0,他引:2
连续三年在相同的繁殖季节,使用齐卡N系母兔148只,分别观察测定了四种不同营养水平组合日粮对不同繁殖状态母兔繁殖能力的影响。结果表明:在延期繁殖(母兔断奶后再配种)条件下,不同能量、蛋白水平对母兔繁殖性能的影响差异不显著(P>0。05),但中能高蛋白(DE10.88兆焦/公斤、CP19%)的第3组日粮略胜于其它组合。由此得出,在引延期繁殖的ZIKA能繁母兔,适宜的营养水平为:消化能(DE)10兆焦/公斤,粗蛋白(CP)19%,粗纤维(CF)10%。在母兔进行半频密繁殖(母兔产后第14天开始配种)情况下,高能高蛋白(CDE12.13兆焦/公斤、CP19%)饲粮对提高繁殖性能的效果明显优于高能低蛋(DE12.13兆焦/公斤,CP15%)和低能低蛋白(DE10.88兆焦/公斤)和CP15%)饲粮(P<0.05),尤其对提高母兔泌乳力效果明显(P<0;01),因此,实行频密(产后立即配种)或半频密繁殖的母兔,适宜营养水平为消化能12兆焦繁殖的母兔,适宜营养水平为消化能12兆焦/公斤,粗蛋白19%,粗纤维9-12%. 相似文献