Anatid herpesvirus 1 CH virulent strain induces syncytium and apoptosis in duck embryo fibroblast cultures

Anatid herpesvirus 1 (AHV-1) CH virulent strain was first isolated from an infected duck and it was found that this virus strain could induce cytopathic effect (CPE) in duck embryo fibroblast (DEF). Following AHV-1 infection, DEF showed morphological changes such as cell rounding, improved refractivity and detachment from the culture surface. However, its pathological characteristics were not adequately known. Related studies were performed and the results showed that syncytium formation could be observed as the other type of CPE in AHV-1 infection. Hematoxylin-eosin staining and 4’, 6-diamidino-2-phenylindole (DAPI) staining of infected DEF were each used to visualize the shape and distribution of chromatin within nuclei and nuclear fragmentation was observed. Chromatin condensation and margination, as well as formation of apoptotic bodies were observed by transmission electron microscopy (TEM). DNA ladder formation was detected in AHV-1 infected cells and apoptosis of the infected DEF was also detected by flow cytometry analysis of Annexin V-FITC/PI staining method. Therefore, it was suggested that AHV-1 virulent strain can induce syncytium and apoptosis in DEF. Syncytium formation and apoptosis observed in this study may contribute to the elucidation of AHV-1 pathogenesis.     

绿色管理在园林绿化产业中的运用

从绿色管理的概念出发,在研究园林绿化产业巨大的环境保护与生态效益的基础之上,探讨了将绿色管理思想运用到园林绿化产业中,并从园林绿化设计、施工、养护、废弃物处理4个环节具体分析了绿色管理在园林绿化产业的运用.     

对森林生态共享数据问题的初探

共享数据可以为我国经济社会可持续发展指标研究提供丰富的支撑。可持续发展指标的系统调查已经成为各级行政主管部门的经常性工作,数据的获得成为很容易的事情,并且形成惯例成为数字化产品序列,使指标的实施有了可操作的定量化方法。     

激素与营养液配施对平菇产量及品质的影响

植物激素与营养液配合施用可促进平菇菌丝生长和子实体发育，提高产量，改善商品性状，但却不能增加干物质的积累。对营养成分含量的影响随激素与营养液配方的不同而异。     

白牦牛早期胚胎发育条件的初步研究

研究了卵母细胞质量和体外受精卵培养温度对白牦牛早期胚胎发育的影响。结果表明:白牦牛体外受精时要选择A级质量的卵母细胞,体外受精胚胎发育的最适温度为38℃。为完善白牦牛胚胎的体外生产体系提供了实验依据。     

Field evaluation of insect-resistant transgenic Populus nigra trees

The performance of insect-resistant transgenic poplar trees (Populusnigra) expressing a Cry1Ac gene from Bacillus thuringiensis subsp. Kurstaki HD-1 against poplar defoliators was evaluated in the field at the Manas Forest Station in Xinjiang Uygur Autonomous Region during1994–1997. The results showed that the average percentage of highly damaged leaves on the transgenic trees was 10% while that on the control trees in nearby plantations reached 80–90%. The average number of pupae per m² of soil at 20cm depth in transgenic poplar plantation was 18 which was only 20% of that found in the non-transgenic control field. The number of pupae and the leaf-damage on transgenic trees described above are all far below the threshold set for chemical protection measures. The non-transformed poplar trees grown in the same plantation with the transgenic trees were also protected indicating that cross protection occurred between these two kinds of trees. Insect-resistant transgenic poplar trees have a potential application value in afforestation. This revised version was published online in August 2006 with corrections to the Cover Date.     

Effect of stachyose supplementation on growth performance, nutrient digestibility and caecal fermentation characteristics in broilers

1. The objective of this study was to evaluate whether the oligosaccharide stachyose enhances gastrointestinal tract health by fermentation and proliferation of desirable bacteria species and thus affects growth performance and nutrient digestibility in broilers. 2. A total of 432 1-d-old male Arbor Acres (AA) broilers were randomly allocated to one of 6 treatments, with 12 replicate pens per treatment and 6 birds per pen. Chicks were fed a maize-hamlet protein 300 (HP300) basal diet with 0, 4.0, 8.0, 12.0 or 16.0 g/kg stachyose. A sixth diet contained no HP300 but soybean meal (SBM) and provided 8.7 g/kg stachyose and 3.1 g/kg raffinose. The duration of the study was 42 d. 3. Stachyose contents above 12.0 g/kg depressed group body weights, average daily gain and feed/gain but not feed intake during the whole experimental period. Broiler growth decreased linearly and quadratically with increasing stachyose content. No differences were detected between diets supplemented with 12.0 g/kg stachyose and SBM. 4. Nutrient digestibility tended to decrease but not significantly with increasing stachyose. 5. Stachyose content had no significant positive effects on caecal pH, microflora population and the resulting short-chain fatty acid (SCFA) metabolites during the 42 d experiment, with only butyrate differing significantly in the initial period.     

关中灌区粮食生产“两高一优”机械化模式

根据关中灌区自然经济条件，提出了一种新的机械化模式。经过９年实践表明，采用该模式，无论丰年、平年、灾年均可大幅度增产，能提高抵抗自然灾害的能力，使丰、灾年间产量波幅减小，更趋稳定。     

First report of Pseudomonas aeruginosa causing tumor disease of Populus koreana in China

Journal of Plant Diseases and Protection - In May 2018, a bacterial tumor disease was observed on Populus koreana in Shuangyang county, Changchun city, Jilin Province. Based on physiological...