排序方式: 共有41条查询结果,搜索用时 15 毫秒
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Simone Peletto Matteo Perucchini Cristina Ac��n Mark P. Dalgleish Hugh W. Reid Roberto Rasero Paola Sacchi Paula Stewart Maria Caramelli Ezio Ferroglio Elena Bozzetta Daniela Meloni Riccardo Orusa Serena Robetto Silvia Gennero Wilfred Goldmann Pier Luigi Acutis 《Journal of veterinary science (Suw?n-si, Korea)》2009,10(2):115-120
The genetics of the prion protein gene (PRNP) play a crucial role in determining the relative susceptibility to transmissible spongiform encephalopathies (TSEs) in several mammalian species. To determine the PRNP gene variability in European red deer (Cervus elaphus), roe deer (Capreolus capreolus) and chamois (Rupicapra rupicapra), the PRNP open reading frame from 715 samples was analysed to reveal a total of ten single nucleotide polymorphisms (SNPs). In red deer, SNPs were found in codons 15, 21, 59, 78, 79, 98, 136, 168 and 226. These polymorphisms give rise to 12 haplotypes, and one of which is identical to the PRNP of American wapiti (Rocky Mountain elk, Cervus elaphus nelsoni). One silent mutation at codon 119 was detected in chamois and no SNPs were found in roe deer. This analysis confirmed that European wild ruminants have a PRNP genetic background that is compatible with TSE susceptibility, including chronic wasting disease. 相似文献
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Marita JM Vermerris W Ralph J Hatfield RD 《Journal of agricultural and food chemistry》2003,51(5):1313-1321
Most studies published thus far on the four brown midrib (bm) mutants (bm1, bm2, bm3, and bm4) in maize (Zea mays L.) have focused on one or two individual mutants, and comparisons between studies have been difficult because of variation in genetic backgrounds, maturity, and source of tissue. Detailed analyses of the stalks of the four bm single mutants and a bm1-bm2 double mutant in a common genetic background (inbred A619) revealed structural and compositional changes in their isolated cell walls and lignins compared to the wild-type inbred. 2D-NMR revealed a significant presence of benzodioxane units in the bm3 isolated lignin. 1D (13)C NMR revealed increased aldehyde levels in the bm1 and bm1-bm2 mutants compared to the wild-type inbred. The bm3 and bm1-bm2 mutants contained less Klason lignin in the isolated cell walls. The bm1, bm3, and bm1-bm2 mutants contained approximately 50% less esterified p-coumaric acid with noticeably elevated levels of ferulate in the bm3 mutant. A difference among bm mutants in the solubility of p-coumaric acid-lignin complexes during cellulase enzyme treatment was also discovered, suggesting that the bm mutations might also differ in the structural organization of lignin. 相似文献
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Savannas worldwide are vital for both socioeconomic and biodiversity values. In these ecosystems, management decisions are based on the perception that wildlife and livestock compete for food, yet there are virtually no experimental data to support this assumption. We examined the effects of wild African ungulates on cattle performance, food intake, and diet quality. Wild ungulates depressed cattle food intake and performance during the dry season (competition) but enhanced cattle diet quality and performance during the wet season (facilitation). These results extend our understanding of the context-dependent-competition-facilitation balance, in general, and are critical for better understanding and managing wildlife-livestock coexistence in human-occupied savanna landscapes. 相似文献
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Wilfred O Alegbeleye Sam O Obasa Olugbenga O Olude Kehinde Otubu Wahab Jimoh 《Aquaculture Research》2012,43(3):412-420
A preliminary feeding trial was conducted to evaluate the nutritive value of adult variegated grasshopper (Zonocerus variegatus) meal (VGM) as a replacement for fish meal in the diets of Clarias gariepinus fingerlings. Proximate analysis result showed that VGM had 61.50% crude protein, 6.87% crude lipid, 27.33 NFE and 9.35% chitin. Five isonitrogenous (30%) and isoenergetic (18.42 MJ kg?1) diets were formulated in which VGM progressively replaced fishmeal at five inclusion levels (0%, 25%, 50%, 75% and 100%). The best performance in terms of % weight gain was observed in the group of fish fed ED1 (25% inclusion level) and was not significantly different (P>0.05) from the groups fed the control diet. Weight gain and specific growth rate were significantly lower (P<0.05) in the group fed ED4 (100%VGM). Feed conversion ratio and protein efficiency ratio were numerically but not significantly different (P>0.05) among the different groups. The results indicate that apparent protein and lipid digestibility were high at all levels, although there were decreases with an increase in the inclusion levels. Carcass lipid decreased with an increase in the inclusion level of VGM. The VGM can replace fish meal up to 25% in the diets of C. gariepinus fingerlings without any adverse effect on growth and nutrient utilization. 相似文献
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WW Metcalf BM Griffin RM Cicchillo J Gao SC Janga HA Cooke BT Circello BS Evans W Martens-Habbena DA Stahl WA van der Donk 《Science (New York, N.Y.)》2012,337(6098):1104-1107
Relative to the atmosphere, much of the aerobic ocean is supersaturated with methane; however, the source of this important greenhouse gas remains enigmatic. Catabolism of methylphosphonic acid by phosphorus-starved marine microbes, with concomitant release of methane, has been suggested to explain this phenomenon, yet methylphosphonate is not a known natural product, nor has it been detected in natural systems. Further, its synthesis from known natural products would require unknown biochemistry. Here we show that the marine archaeon Nitrosopumilus maritimus encodes a pathway for methylphosphonate biosynthesis and that it produces cell-associated methylphosphonate esters. The abundance of a key gene in this pathway in metagenomic data sets suggests that methylphosphonate biosynthesis is relatively common in marine microbes, providing a plausible explanation for the methane paradox. 相似文献
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Okafor Simeon Chibuko Ogugua Akwoba Joseph Ihedioha John Ikechukwu Onunkwo Joseph Ikechukwu Ezenduka Ekene Vivienne Okafor Uju Catherine Ezema Wilfred Sunday 《Veterinary research communications》2022,46(2):517-526
Veterinary Research Communications - This study evaluated the prevalence, hematological and some biochemical alterations in Brucella seropositive Muturu breed of cattle which may change the... 相似文献
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