寡糖诱导向日葵抗锈病的信号转导途径

为了解寡糖诱导向日葵抗锈病过程中的信号转导途径,利用寡糖、水杨酸（SA）、茉莉酸（JA）预先喷雾处理向日葵叶片,1d后接种锈菌,通过对植株体内脂氧合酶（LOX）活性及SA含量的测定,并通过RT-PCR对SA、JA信号途径的标记基因PR5和PDF1.2等表达水平进行测定,初步确定诱导抗性表达的信号转导途径。结果表明：经寡糖诱导后,向日葵叶片内LOX活性降低,SA含量上升,且诱导水杨酸途径标记基因PR5表达量增加。结果说明寡糖诱导向日葵抵抗锈病主要与SA介导的信号途径有关。     

Effects of glucose and Mg2+ in the neurons damaged by glutamate

AIM and METHODS: To observe the effects of glucose-free and Mg²⁺-free in the extracellular fluid on the changes of [Ca ²⁺]_i in the cerebro-cortical neurons damaged by 1mmol/L glutamate using laser confocal scanning microscope. RESULTS: Both frequency and amplitude of neuronal calcium oscillation induced by glutamate were lowered in glucose-free and Mg²⁺-free buffers. The basic [Ca²⁺]_i concentration was lowered in the former case , but it was elevated in the latter case. CONCLUSION: Mg²⁺-free aggravates [Ca²⁺]_i overload induced by 1mmol/L glutamate ,under certain conditions the glucose-free might resist damage role of glutamate and Mg²⁺-free.     

14份小麦种质资源抗麦长管蚜遗传多样性的SSR分析

利用SSR分子标记在分子水平探讨小麦种质资源抗麦长管蚜的遗传多样性,为高效节本型和环境友好型的抗蚜育种的研究和利用提供理论依据和技术支持。结果表明：在小麦的A、B和D 3组同源染色体组上的175对SSR分子标记鉴定出了有多态性的32对引物,其中在D同源组中小麦抗蚜性的遗传多样性较高,同时在21对染色体中7D染色体上遗传...     

Effects of temperature and humidity on the contact angle of pesticide droplets on rice leaf surfaces

The effects of external factors such as temperature, humidity, pesticide formulation, and pesticide concentration on the contact angle of pesticide droplets on rice leaf surfaces were analyzed. The experiments showed that there were significant differences in the contact angles of droplets on the leaf surfaces under different temperatures and humidity. As the ambient temperature increased, the contact angle first decreased and then increased, reaching a minimum value at 25°C. With a gradual increase in humidity, the contact angle significantly increased and reached a maximum at 100% humidity. Finally, it was concluded that both the formulation and concentration of the pesticide had a significant effect on the contact angle of droplets on rice leaf surfaces. The experiments also illustrated that the effects of the pesticide formulation and concentration on the contact angle were more significant than those of temperature and humidity.     

成都平原超级稻超高产群体产量形成与物质生产特点研究

对Ⅱ优602、德香4103、Q优8号等3个超级稻品种不同产量级别群体的产量形成及干物质生产特点进行了研究。结果表明,超级稻品种从中产(产量≤9.0 t/hm2)实现高产(产量9.0~10.5 t/hm2)关键在于每穗粒数提高后库容的增大,并同时提高结实率;而要实现超高产(产量≥10.5 t/hm2)则有赖于大库条件下结实率的进一步提高。此外,超级稻品种产量分别与分蘖临界期后各阶段的干物质积累量存在显著或极显著的正相关关系,超级稻品种高产群体获得高产的关键是提高抽穗后物质生产能力,而要进一步实现超高产则需要抽穗前后干物质生产能力的协同提高。     

8个乌塌菜品种比较研究

在收集的乌塌菜品种中筛选出表现较好的8个品种进行比较试验,从生育期、主要农艺性状、产量、主要营养品质及苗期抗虫性等方面进行了初步研究。结果表明,矮塌型黑心乌(W2)、淮南塌地乌6号(W39)和优选黄芽14(W56)的生育期较短(67d左右)、产量和主要营养品质较好、苗期虫害相对较少,可初步确定适合在江淮流域种植。     

附子-甘草对离体蛙心的影响及其指纹图谱研究

目的 研究附子-甘草合煎液和混合液对离体蛙心的影响及其物质基础。方法 通过离体蛙心实验观察附子-甘草合煎液和混合液对离体正常蛙心和心衰蛙心功能的影响,应用高效液相色谱法建立并测定附子-甘草煎液的HPLC指纹图谱。结果 附子-甘草合煎液与混合液的强心作用有差别;附子与甘草配伍后其指标成分的含量发生了明显变化,且合煎液与混合液的成分变化差别显著。结论 附子-甘草合煎液和混合液对离体蛙心的作用及其指纹图谱均不一致。     

江西柑桔园植绥螨简记

本文调查和鉴定了江西省柑桔园的植绥螨,共有6属14种。列出了分属、种检索表;并对重要种类尼氏真绥螨和香港植绥螨的形态和习性作了描述。     

转基因香石竹Moonlite品系特异性定性PCR检测方法的建立

以转基因香石竹品种Moonlite(月之伊人)为研究对象,通过TAIL-PCR方法,获得外源基因插入位点的左侧旁邻序列。根据旁邻序列设计引物,建立品系特异性定性PCR方法,其扩增片段覆盖了转化载体及香石竹基因组临界序列。本方法特异性好、灵敏度高,可快速、准确、高效地检测转基因香石竹Moonlite品种。     

农业院校校外规模化试验示范基地建设思考

规模化试验示范基地承载着农业院校科技创新、人才培养、技术示范与推广的任务。文章结合校外规模化试验示范基地的实际,阐述了校外基地建设的必要性,介绍了华中农业大学校外规模化试验示范基地概况,并提出了提高对校外试验示范基地建设的认识、做好基地的整体规划、理顺管理体制等促进校外规模化试验示范基地建设的建议。